Reference spectra from squamous epithelium and connective tissue allow whole section proteomics analysis

M. Roesch-Ely, M. Schnölzer, Matthias Nees, P.K. Plinkert, F.X. Bosch (Corresponding Author)

Research output: Contribution to journalArticle

Abstract

We reasoned that micro-dissection of tumour cells for protein expression studies should be omitted since tumour-stroma interactions are an important part of the biology of solid tumours. To study such interactions in head and neck squamous cell carcinoma (HNSCC) development, we generated reference protein spectra for normal squamous epithelium and connective tissue by SELDI-TOF-MS. Calgranulins A and B, Annexin1 and Histone H4 were found to be strongly enriched in the epithelium. The alpha-defensins 1-3 and the haemoglobin subunits were identified in the connective tissue. Tumour-distant epithelia, representing early pre-malignant lesions, showed up-regulated expression of the stromal alpha-defensins, whereas the epithelial Annexin 1 was down-regulated. Thus, tumour microenvironment interactions occur very early in the carcinogenic process. These data demonstrate that omitting micro-dissection is actually beneficial for studying changes in protein expression during development and progression of solid tumours.
Original languageEnglish
Pages (from-to)218-226
JournalArchives of Physiology and Biochemistry
Volume116
Issue number4-5
DOIs
Publication statusPublished - 2010
MoE publication typeA1 Journal article-refereed

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Keywords

  • Proteomics
  • HNSCC
  • tumour microenvironment
  • field cancerization
  • protein biomarkers

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