Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 218-226 |
| Journal | Archives of Physiology and Biochemistry |
| Volume | 116 |
| Issue number | 4-5 |
| DOIs | |
| Publication status | Published - 2010 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- Proteomics
- HNSCC
- tumour microenvironment
- field cancerization
- protein biomarkers
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Reference spectra from squamous epithelium and connective tissue allow whole section proteomics analysis. / Roesch-Ely, M.; Schnölzer, M.; Nees, Matthias; Plinkert, P.K.; Bosch, F.X. (Corresponding Author).
In: Archives of Physiology and Biochemistry, Vol. 116, No. 4-5, 2010, p. 218-226.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Reference spectra from squamous epithelium and connective tissue allow whole section proteomics analysis
AU - Roesch-Ely, M.
AU - Schnölzer, M.
AU - Nees, Matthias
AU - Plinkert, P.K.
AU - Bosch, F.X.
PY - 2010
Y1 - 2010
N2 - We reasoned that micro-dissection of tumour cells for protein expression studies should be omitted since tumour-stroma interactions are an important part of the biology of solid tumours. To study such interactions in head and neck squamous cell carcinoma (HNSCC) development, we generated reference protein spectra for normal squamous epithelium and connective tissue by SELDI-TOF-MS. Calgranulins A and B, Annexin1 and Histone H4 were found to be strongly enriched in the epithelium. The alpha-defensins 1-3 and the haemoglobin subunits were identified in the connective tissue. Tumour-distant epithelia, representing early pre-malignant lesions, showed up-regulated expression of the stromal alpha-defensins, whereas the epithelial Annexin 1 was down-regulated. Thus, tumour microenvironment interactions occur very early in the carcinogenic process. These data demonstrate that omitting micro-dissection is actually beneficial for studying changes in protein expression during development and progression of solid tumours.
AB - We reasoned that micro-dissection of tumour cells for protein expression studies should be omitted since tumour-stroma interactions are an important part of the biology of solid tumours. To study such interactions in head and neck squamous cell carcinoma (HNSCC) development, we generated reference protein spectra for normal squamous epithelium and connective tissue by SELDI-TOF-MS. Calgranulins A and B, Annexin1 and Histone H4 were found to be strongly enriched in the epithelium. The alpha-defensins 1-3 and the haemoglobin subunits were identified in the connective tissue. Tumour-distant epithelia, representing early pre-malignant lesions, showed up-regulated expression of the stromal alpha-defensins, whereas the epithelial Annexin 1 was down-regulated. Thus, tumour microenvironment interactions occur very early in the carcinogenic process. These data demonstrate that omitting micro-dissection is actually beneficial for studying changes in protein expression during development and progression of solid tumours.
KW - Proteomics
KW - HNSCC
KW - tumour microenvironment
KW - field cancerization
KW - protein biomarkers
U2 - 10.3109/13813455.2010.525240
DO - 10.3109/13813455.2010.525240
M3 - Article
VL - 116
SP - 218
EP - 226
JO - Archives of Physiology and Biochemistry
JF - Archives of Physiology and Biochemistry
SN - 1381-3455
IS - 4-5
ER -