Regioselective deacetylation of cellulose acetates by acetyl xylan esterases of different CE-families

Clemens Altaner, Bodo Saake, Maija Tenkanen, Jaime Eyzaguirre, Craig B. Faulds, Peter Biely, Liisa Viikari, Matti Siika-aho, Jurgen Puls (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Cellulose acetate (CA) was found to be a substrate of several acetyl xylan esterases (AXE). Eight AXE from different carbohydrate esterase (CE) families were tested on their activity against CA with a degree of substitution of 0.7 and 1.4. The classification of the AXEs into CE families according to their structure by hydrophobic cluster analysis followed clearly their activity against CA. Within the same CE family similar, and between the CE families different deacetylation behaviours could be observed. Furthermore, each esterase family showed a distinct regioselective mode of action. The CE 1 family enzymes regioselectively cleaved the substituents in C2- and C3-position, while CE 5 family enzymes only cleaved the acetyl groups in C2-position. CE 4 family enzymes seemed to interact only with the substituents in C3-position. Evidence was found that the deacetylation reaction of the CE 1 family enzymes proceeded faster in C2- than in C3-position of CA. The enzymes were able to cleave acetyl groups from fully substituted anhydroglucose units.
Original languageEnglish
Pages (from-to)95-104
Number of pages10
JournalJournal of Biotechnology
Volume105
Issue number1-2
DOIs
Publication statusPublished - 2003
MoE publication typeA1 Journal article-refereed

Fingerprint

acetylxylan esterase
Esterases
Carbohydrates
Cellulose
Enzymes
acetylcellulose
Xylans
Cluster analysis

Keywords

  • Cellulose acetate
  • Acetyl xylan esterase
  • Regioselective deacetylation
  • CE families
  • 13C-NMR-spectroscopy

Cite this

Altaner, C., Saake, B., Tenkanen, M., Eyzaguirre, J., Faulds, C. B., Biely, P., ... Puls, J. (2003). Regioselective deacetylation of cellulose acetates by acetyl xylan esterases of different CE-families. Journal of Biotechnology, 105(1-2), 95-104. https://doi.org/10.1016/S0168-1656(03)00187-1
Altaner, Clemens ; Saake, Bodo ; Tenkanen, Maija ; Eyzaguirre, Jaime ; Faulds, Craig B. ; Biely, Peter ; Viikari, Liisa ; Siika-aho, Matti ; Puls, Jurgen. / Regioselective deacetylation of cellulose acetates by acetyl xylan esterases of different CE-families. In: Journal of Biotechnology. 2003 ; Vol. 105, No. 1-2. pp. 95-104.
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title = "Regioselective deacetylation of cellulose acetates by acetyl xylan esterases of different CE-families",
abstract = "Cellulose acetate (CA) was found to be a substrate of several acetyl xylan esterases (AXE). Eight AXE from different carbohydrate esterase (CE) families were tested on their activity against CA with a degree of substitution of 0.7 and 1.4. The classification of the AXEs into CE families according to their structure by hydrophobic cluster analysis followed clearly their activity against CA. Within the same CE family similar, and between the CE families different deacetylation behaviours could be observed. Furthermore, each esterase family showed a distinct regioselective mode of action. The CE 1 family enzymes regioselectively cleaved the substituents in C2- and C3-position, while CE 5 family enzymes only cleaved the acetyl groups in C2-position. CE 4 family enzymes seemed to interact only with the substituents in C3-position. Evidence was found that the deacetylation reaction of the CE 1 family enzymes proceeded faster in C2- than in C3-position of CA. The enzymes were able to cleave acetyl groups from fully substituted anhydroglucose units.",
keywords = "Cellulose acetate, Acetyl xylan esterase, Regioselective deacetylation, CE families, 13C-NMR-spectroscopy",
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Altaner, C, Saake, B, Tenkanen, M, Eyzaguirre, J, Faulds, CB, Biely, P, Viikari, L, Siika-aho, M & Puls, J 2003, 'Regioselective deacetylation of cellulose acetates by acetyl xylan esterases of different CE-families', Journal of Biotechnology, vol. 105, no. 1-2, pp. 95-104. https://doi.org/10.1016/S0168-1656(03)00187-1

Regioselective deacetylation of cellulose acetates by acetyl xylan esterases of different CE-families. / Altaner, Clemens; Saake, Bodo; Tenkanen, Maija; Eyzaguirre, Jaime; Faulds, Craig B.; Biely, Peter; Viikari, Liisa; Siika-aho, Matti; Puls, Jurgen (Corresponding Author).

In: Journal of Biotechnology, Vol. 105, No. 1-2, 2003, p. 95-104.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Regioselective deacetylation of cellulose acetates by acetyl xylan esterases of different CE-families

AU - Altaner, Clemens

AU - Saake, Bodo

AU - Tenkanen, Maija

AU - Eyzaguirre, Jaime

AU - Faulds, Craig B.

AU - Biely, Peter

AU - Viikari, Liisa

AU - Siika-aho, Matti

AU - Puls, Jurgen

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N2 - Cellulose acetate (CA) was found to be a substrate of several acetyl xylan esterases (AXE). Eight AXE from different carbohydrate esterase (CE) families were tested on their activity against CA with a degree of substitution of 0.7 and 1.4. The classification of the AXEs into CE families according to their structure by hydrophobic cluster analysis followed clearly their activity against CA. Within the same CE family similar, and between the CE families different deacetylation behaviours could be observed. Furthermore, each esterase family showed a distinct regioselective mode of action. The CE 1 family enzymes regioselectively cleaved the substituents in C2- and C3-position, while CE 5 family enzymes only cleaved the acetyl groups in C2-position. CE 4 family enzymes seemed to interact only with the substituents in C3-position. Evidence was found that the deacetylation reaction of the CE 1 family enzymes proceeded faster in C2- than in C3-position of CA. The enzymes were able to cleave acetyl groups from fully substituted anhydroglucose units.

AB - Cellulose acetate (CA) was found to be a substrate of several acetyl xylan esterases (AXE). Eight AXE from different carbohydrate esterase (CE) families were tested on their activity against CA with a degree of substitution of 0.7 and 1.4. The classification of the AXEs into CE families according to their structure by hydrophobic cluster analysis followed clearly their activity against CA. Within the same CE family similar, and between the CE families different deacetylation behaviours could be observed. Furthermore, each esterase family showed a distinct regioselective mode of action. The CE 1 family enzymes regioselectively cleaved the substituents in C2- and C3-position, while CE 5 family enzymes only cleaved the acetyl groups in C2-position. CE 4 family enzymes seemed to interact only with the substituents in C3-position. Evidence was found that the deacetylation reaction of the CE 1 family enzymes proceeded faster in C2- than in C3-position of CA. The enzymes were able to cleave acetyl groups from fully substituted anhydroglucose units.

KW - Cellulose acetate

KW - Acetyl xylan esterase

KW - Regioselective deacetylation

KW - CE families

KW - 13C-NMR-spectroscopy

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DO - 10.1016/S0168-1656(03)00187-1

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