Regulation of alpha-amylase promoter by gibberellic acid and abscisic acid in barley protoplasts transformed by electroporation

Marjatta Salmenkallio, Riitta Hannus, Teemu Teeri, Veli Kauppinen

Research output: Contribution to journalArticleScientificpeer-review

21 Citations (Scopus)

Abstract

Transient gene expression was studied in isolated protoplasts of barley (Hordeum vulgare L. cv Himalaya) transformed by electroporation. Two plasmid constructions were used, both of which contained the gene coding for neomycin phosphotransferase II (nptII) as a reporter gene. In one plasmid the reporter gene was under the control of an α-amylase group 1 gene promoter of barley and in the other, used as a control, under the CaMV 35S transcript promoter. Protoplasts were isolated from three different types of tissue: the aleurone layer, the scutellar epithelium and the mesophyll. All three types of protoplasts electroporated with 35S -nptII plasmid construction showed strong NPTII activity on which GA3 and ABA had no effect. In protoplasts isolated from the aleurone layer and scutellum the expression of amy-nptII was low when compared with the expression of 35S -nptII. In aleurone protoplasts GA3 enhanced the expression of amy-nptII about tenfold and ABA prevented the action of GA3. In protoplasts isolated from the scutellar epithelium GA3 did not affect the low level of expression of amy-nptII. In mesophyll protoplasts the amy-nptII was not expressed at all.
Original languageEnglish
Pages (from-to)352-355
JournalPlant Cell Reports
Volume9
Issue number7
DOIs
Publication statusPublished - 1990
MoE publication typeA1 Journal article-refereed

Fingerprint

kanamycin kinase
electroporation
gibberellic acid
alpha-amylase
protoplasts
abscisic acid
barley
promoter regions
aleurone layer
plasmids
reporter genes
mesophyll
epithelium
amylases
Hordeum vulgare
genes
gene expression

Cite this

Salmenkallio, Marjatta ; Hannus, Riitta ; Teeri, Teemu ; Kauppinen, Veli. / Regulation of alpha-amylase promoter by gibberellic acid and abscisic acid in barley protoplasts transformed by electroporation. In: Plant Cell Reports. 1990 ; Vol. 9, No. 7. pp. 352-355.
@article{01a1a38b8a314acd8040d1f28adc2e7e,
title = "Regulation of alpha-amylase promoter by gibberellic acid and abscisic acid in barley protoplasts transformed by electroporation",
abstract = "Transient gene expression was studied in isolated protoplasts of barley (Hordeum vulgare L. cv Himalaya) transformed by electroporation. Two plasmid constructions were used, both of which contained the gene coding for neomycin phosphotransferase II (nptII) as a reporter gene. In one plasmid the reporter gene was under the control of an α-amylase group 1 gene promoter of barley and in the other, used as a control, under the CaMV 35S transcript promoter. Protoplasts were isolated from three different types of tissue: the aleurone layer, the scutellar epithelium and the mesophyll. All three types of protoplasts electroporated with 35S -nptII plasmid construction showed strong NPTII activity on which GA3 and ABA had no effect. In protoplasts isolated from the aleurone layer and scutellum the expression of amy-nptII was low when compared with the expression of 35S -nptII. In aleurone protoplasts GA3 enhanced the expression of amy-nptII about tenfold and ABA prevented the action of GA3. In protoplasts isolated from the scutellar epithelium GA3 did not affect the low level of expression of amy-nptII. In mesophyll protoplasts the amy-nptII was not expressed at all.",
author = "Marjatta Salmenkallio and Riitta Hannus and Teemu Teeri and Veli Kauppinen",
year = "1990",
doi = "10.1007/BF00232397",
language = "English",
volume = "9",
pages = "352--355",
journal = "Plant Cell Reports",
issn = "0721-7714",
publisher = "Springer",
number = "7",

}

Regulation of alpha-amylase promoter by gibberellic acid and abscisic acid in barley protoplasts transformed by electroporation. / Salmenkallio, Marjatta; Hannus, Riitta; Teeri, Teemu; Kauppinen, Veli.

In: Plant Cell Reports, Vol. 9, No. 7, 1990, p. 352-355.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Regulation of alpha-amylase promoter by gibberellic acid and abscisic acid in barley protoplasts transformed by electroporation

AU - Salmenkallio, Marjatta

AU - Hannus, Riitta

AU - Teeri, Teemu

AU - Kauppinen, Veli

PY - 1990

Y1 - 1990

N2 - Transient gene expression was studied in isolated protoplasts of barley (Hordeum vulgare L. cv Himalaya) transformed by electroporation. Two plasmid constructions were used, both of which contained the gene coding for neomycin phosphotransferase II (nptII) as a reporter gene. In one plasmid the reporter gene was under the control of an α-amylase group 1 gene promoter of barley and in the other, used as a control, under the CaMV 35S transcript promoter. Protoplasts were isolated from three different types of tissue: the aleurone layer, the scutellar epithelium and the mesophyll. All three types of protoplasts electroporated with 35S -nptII plasmid construction showed strong NPTII activity on which GA3 and ABA had no effect. In protoplasts isolated from the aleurone layer and scutellum the expression of amy-nptII was low when compared with the expression of 35S -nptII. In aleurone protoplasts GA3 enhanced the expression of amy-nptII about tenfold and ABA prevented the action of GA3. In protoplasts isolated from the scutellar epithelium GA3 did not affect the low level of expression of amy-nptII. In mesophyll protoplasts the amy-nptII was not expressed at all.

AB - Transient gene expression was studied in isolated protoplasts of barley (Hordeum vulgare L. cv Himalaya) transformed by electroporation. Two plasmid constructions were used, both of which contained the gene coding for neomycin phosphotransferase II (nptII) as a reporter gene. In one plasmid the reporter gene was under the control of an α-amylase group 1 gene promoter of barley and in the other, used as a control, under the CaMV 35S transcript promoter. Protoplasts were isolated from three different types of tissue: the aleurone layer, the scutellar epithelium and the mesophyll. All three types of protoplasts electroporated with 35S -nptII plasmid construction showed strong NPTII activity on which GA3 and ABA had no effect. In protoplasts isolated from the aleurone layer and scutellum the expression of amy-nptII was low when compared with the expression of 35S -nptII. In aleurone protoplasts GA3 enhanced the expression of amy-nptII about tenfold and ABA prevented the action of GA3. In protoplasts isolated from the scutellar epithelium GA3 did not affect the low level of expression of amy-nptII. In mesophyll protoplasts the amy-nptII was not expressed at all.

U2 - 10.1007/BF00232397

DO - 10.1007/BF00232397

M3 - Article

VL - 9

SP - 352

EP - 355

JO - Plant Cell Reports

JF - Plant Cell Reports

SN - 0721-7714

IS - 7

ER -