Release of small phenolic compounds from Brewer's spent grain and its lignin fractions by human intestinal microbiota in vitro

Anna-Maija Aura (Corresponding Author), Piritta Niemi, Ismo Mattila, Klaus Niemelä, Annika Smeds, Tarja Tamminen, Craig Faulds, Johanna Buchert, Kaisa Poutanen

Research output: Contribution to journalArticleScientificpeer-review

10 Citations (Scopus)

Abstract

Brewer's spent grain (BSG), the major side-stream from brewing, is rich in protein, lignin, and nonstarch polysaccharides. Lignin is a polyphenolic macromolecule considered resilient toward breakdown and utilization by colon microbiota, although some indications of release of small phenolic components from lignin in animals have been shown. The aim of this study was to investigate if the human intestinal microbiota can release lignans and small phenolic compounds from whole BSG, a lignin-enriched insoluble fraction from BSG and a deferuloylated fraction, in a metabolic in vitro colon model. The formation of short-chain fatty acid (SCFA) was also investigated. More lignin-related monomers and dilignols were detected from the lignin-enriched fraction than from BSG or deferuloylated BSG. SCFA formation was not suppressed by any of the fractions. It was shown that small lignin-like compounds were released from these samples in the in vitro colon model, originating most likely from lignin.
Original languageEnglish
Pages (from-to)9744-9753
JournalJournal of Agricultural and Food Chemistry
Volume61
Issue number40
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

Fingerprint

spent grains
brewers grains
Lignin
intestinal microorganisms
lignin
phenolic compounds
colon
Colon
Volatile Fatty Acids
short chain fatty acids
Brewing
Lignans
Gastrointestinal Microbiome
In Vitro Techniques
Microbiota
brewing
lignans
Macromolecules
Polysaccharides
polyphenols

Keywords

  • brewer's spent grain
  • in vitro colon model
  • intestinal microbiota
  • lignan
  • lignin

Cite this

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title = "Release of small phenolic compounds from Brewer's spent grain and its lignin fractions by human intestinal microbiota in vitro",
abstract = "Brewer's spent grain (BSG), the major side-stream from brewing, is rich in protein, lignin, and nonstarch polysaccharides. Lignin is a polyphenolic macromolecule considered resilient toward breakdown and utilization by colon microbiota, although some indications of release of small phenolic components from lignin in animals have been shown. The aim of this study was to investigate if the human intestinal microbiota can release lignans and small phenolic compounds from whole BSG, a lignin-enriched insoluble fraction from BSG and a deferuloylated fraction, in a metabolic in vitro colon model. The formation of short-chain fatty acid (SCFA) was also investigated. More lignin-related monomers and dilignols were detected from the lignin-enriched fraction than from BSG or deferuloylated BSG. SCFA formation was not suppressed by any of the fractions. It was shown that small lignin-like compounds were released from these samples in the in vitro colon model, originating most likely from lignin.",
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Release of small phenolic compounds from Brewer's spent grain and its lignin fractions by human intestinal microbiota in vitro. / Aura, Anna-Maija (Corresponding Author); Niemi, Piritta; Mattila, Ismo; Niemelä, Klaus; Smeds, Annika; Tamminen, Tarja; Faulds, Craig; Buchert, Johanna; Poutanen, Kaisa.

In: Journal of Agricultural and Food Chemistry, Vol. 61, No. 40, 2013, p. 9744-9753.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Release of small phenolic compounds from Brewer's spent grain and its lignin fractions by human intestinal microbiota in vitro

AU - Aura, Anna-Maija

AU - Niemi, Piritta

AU - Mattila, Ismo

AU - Niemelä, Klaus

AU - Smeds, Annika

AU - Tamminen, Tarja

AU - Faulds, Craig

AU - Buchert, Johanna

AU - Poutanen, Kaisa

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AB - Brewer's spent grain (BSG), the major side-stream from brewing, is rich in protein, lignin, and nonstarch polysaccharides. Lignin is a polyphenolic macromolecule considered resilient toward breakdown and utilization by colon microbiota, although some indications of release of small phenolic components from lignin in animals have been shown. The aim of this study was to investigate if the human intestinal microbiota can release lignans and small phenolic compounds from whole BSG, a lignin-enriched insoluble fraction from BSG and a deferuloylated fraction, in a metabolic in vitro colon model. The formation of short-chain fatty acid (SCFA) was also investigated. More lignin-related monomers and dilignols were detected from the lignin-enriched fraction than from BSG or deferuloylated BSG. SCFA formation was not suppressed by any of the fractions. It was shown that small lignin-like compounds were released from these samples in the in vitro colon model, originating most likely from lignin.

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