Reliability of destructive leakage detection methods for semirigid retort packages

Eero Hurme, Gun Wirtanen, Lena Axelson-Larsson, Tiina Mattila-Sandholm, Raija Ahvenainen

Research output: Contribution to journalArticleScientificpeer-review

9 Citations (Scopus)

Abstract

Three different destructive testing methods for package integrity (biotesting, dye penetration and electrical conductance) were compared with each other using intact and leaking retort packages. In biotesting, both vegetative cells of Enterobacter aerogenes and spores of Bacillus subtilis were used as test microbes. The biotest results did not significantly differ from each other, most packages with a 50 μm channel leak through the seal area were contaminated. Biotesting with E. aerogenes was regarded as a more favourable method than testing with spores of B. subtilis since it was easier to perform and gas formation in the packages was a simple indicator for product contamination. The electrolytic test was able to discover most of the leaking packages more reliably than the biotests and the dye penetration test. The dye test had similar sensitivity in identifying leaks to that of the biotests. In conclusion, the microbial integrity of packages should not be estimated only by biotests, even if they are generally regarded as the most severe package integrity test methods.
Original languageEnglish
Pages (from-to)203-213
Number of pages11
JournalPackaging Technology and Science
Volume9
Issue number4
DOIs
Publication statusPublished - 1996
MoE publication typeA1 Journal article-refereed

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Coloring Agents
Dyes
Testing
Bacilli
Seals
Contamination
Gases

Cite this

Hurme, Eero ; Wirtanen, Gun ; Axelson-Larsson, Lena ; Mattila-Sandholm, Tiina ; Ahvenainen, Raija. / Reliability of destructive leakage detection methods for semirigid retort packages. In: Packaging Technology and Science. 1996 ; Vol. 9, No. 4. pp. 203-213.
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title = "Reliability of destructive leakage detection methods for semirigid retort packages",
abstract = "Three different destructive testing methods for package integrity (biotesting, dye penetration and electrical conductance) were compared with each other using intact and leaking retort packages. In biotesting, both vegetative cells of Enterobacter aerogenes and spores of Bacillus subtilis were used as test microbes. The biotest results did not significantly differ from each other, most packages with a 50 μm channel leak through the seal area were contaminated. Biotesting with E. aerogenes was regarded as a more favourable method than testing with spores of B. subtilis since it was easier to perform and gas formation in the packages was a simple indicator for product contamination. The electrolytic test was able to discover most of the leaking packages more reliably than the biotests and the dye penetration test. The dye test had similar sensitivity in identifying leaks to that of the biotests. In conclusion, the microbial integrity of packages should not be estimated only by biotests, even if they are generally regarded as the most severe package integrity test methods.",
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Reliability of destructive leakage detection methods for semirigid retort packages. / Hurme, Eero; Wirtanen, Gun; Axelson-Larsson, Lena; Mattila-Sandholm, Tiina; Ahvenainen, Raija.

In: Packaging Technology and Science, Vol. 9, No. 4, 1996, p. 203-213.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Hurme, Eero

AU - Wirtanen, Gun

AU - Axelson-Larsson, Lena

AU - Mattila-Sandholm, Tiina

AU - Ahvenainen, Raija

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AB - Three different destructive testing methods for package integrity (biotesting, dye penetration and electrical conductance) were compared with each other using intact and leaking retort packages. In biotesting, both vegetative cells of Enterobacter aerogenes and spores of Bacillus subtilis were used as test microbes. The biotest results did not significantly differ from each other, most packages with a 50 μm channel leak through the seal area were contaminated. Biotesting with E. aerogenes was regarded as a more favourable method than testing with spores of B. subtilis since it was easier to perform and gas formation in the packages was a simple indicator for product contamination. The electrolytic test was able to discover most of the leaking packages more reliably than the biotests and the dye penetration test. The dye test had similar sensitivity in identifying leaks to that of the biotests. In conclusion, the microbial integrity of packages should not be estimated only by biotests, even if they are generally regarded as the most severe package integrity test methods.

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