Replication regions of two pairs of incompatible lactococcal theta-replicating plasmids

Anne Gravesen, Atte von Wright, Jytte Josephsen, Finn Vogensen (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

26 Citations (Scopus)

Abstract

Incompatibility tests were performed employing 12 replicons belonging to a family of homologous lactococcal theta-replicating plasmids. Two pairs of incompatible plasmids were found, namely, pFV1001 and pFV1201, and pJW565 and pFW094. The replicons of plasmids pFV1001, pFV1201, pJW565, pJW566, and pFW094 were sequenced. Alignments were made of the replicational origins (repA) and putative replication proteins (RepB) of these and 11 related plasmid sequences. Comparison of the alignments with the incompatibility data indicated that the incompatibility determinant could be contained within the 22-bp tandem repeats DRII and/or the inverted repeat IR1 inrepA.In support, the incompatibility determinant of pJW563 was localized to a 743-bp fragment encompassingrepA.A stretch of 13 amino acids of RepB was proposed to be responsible for the plasmid-specific initiation of replication. This stretch is part of a domain containing features that are highly conserved within the proposed DNA binding regions of the initiation proteins from several well-characterized plasmids from Gram-negative bacteria, including pSC101, R6K, and mini-F.

Original languageEnglish
Pages (from-to)115 - 127
Number of pages13
JournalPlasmid
Volume38
Issue number2
DOIs
Publication statusPublished - 1997
MoE publication typeA1 Journal article-refereed

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Plasmids
Replicon
Tandem Repeat Sequences
Gram-Negative Bacteria
Proteins
Amino Acids
DNA

Cite this

Gravesen, A., von Wright, A., Josephsen, J., & Vogensen, F. (1997). Replication regions of two pairs of incompatible lactococcal theta-replicating plasmids. Plasmid, 38(2), 115 - 127. https://doi.org/10.1006/plas.1997.1302
Gravesen, Anne ; von Wright, Atte ; Josephsen, Jytte ; Vogensen, Finn. / Replication regions of two pairs of incompatible lactococcal theta-replicating plasmids. In: Plasmid. 1997 ; Vol. 38, No. 2. pp. 115 - 127.
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Gravesen, A, von Wright, A, Josephsen, J & Vogensen, F 1997, 'Replication regions of two pairs of incompatible lactococcal theta-replicating plasmids', Plasmid, vol. 38, no. 2, pp. 115 - 127. https://doi.org/10.1006/plas.1997.1302

Replication regions of two pairs of incompatible lactococcal theta-replicating plasmids. / Gravesen, Anne; von Wright, Atte; Josephsen, Jytte; Vogensen, Finn (Corresponding Author).

In: Plasmid, Vol. 38, No. 2, 1997, p. 115 - 127.

Research output: Contribution to journalArticleScientificpeer-review

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AU - von Wright, Atte

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AB - Incompatibility tests were performed employing 12 replicons belonging to a family of homologous lactococcal theta-replicating plasmids. Two pairs of incompatible plasmids were found, namely, pFV1001 and pFV1201, and pJW565 and pFW094. The replicons of plasmids pFV1001, pFV1201, pJW565, pJW566, and pFW094 were sequenced. Alignments were made of the replicational origins (repA) and putative replication proteins (RepB) of these and 11 related plasmid sequences. Comparison of the alignments with the incompatibility data indicated that the incompatibility determinant could be contained within the 22-bp tandem repeats DRII and/or the inverted repeat IR1 inrepA.In support, the incompatibility determinant of pJW563 was localized to a 743-bp fragment encompassingrepA.A stretch of 13 amino acids of RepB was proposed to be responsible for the plasmid-specific initiation of replication. This stretch is part of a domain containing features that are highly conserved within the proposed DNA binding regions of the initiation proteins from several well-characterized plasmids from Gram-negative bacteria, including pSC101, R6K, and mini-F.

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