Response of deep subsurface microbial community to different carbon sources and electron acceptors during ~2 months incubation in microcosms

Lotta Purkamo, Malin Bomberg, Mari Nyyssönen, Lasse Ahonen, Ilmo Kukkonen, Merja Itävaara

Research output: Contribution to journalArticleScientificpeer-review

8 Citations (Scopus)

Abstract

Acetate plays a key role as electron donor and acceptor and serves as carbon source in oligotrophic deep subsurface environments. It can be produced from inorganic carbon by acetogenic microbes or through breakdown of more complex organic matter. Acetate is an important molecule for sulfate reducers that are substantially present in several deep bedrock environments. Aceticlastic methanogens use acetate as an electron donor and/or a carbon source. The goal of this study was to shed light on carbon cycling and competition in microbial communities in fracture fluids of Finnish crystalline bedrock groundwater system. Fracture fluid was anaerobically collected from a fracture zone at 967 m depth of the Outokumpu Deep Drill Hole and amended with acetate, acetate + sulfate, sulfate only or left unamended as a control and incubated up to 68 days. The headspace atmosphere of microcosms consisted of 80% hydrogen and 20% CO2. We studied the changes in the microbial communities with community fingerprinting technique as well as high-throughput 16S rRNA gene amplicon sequencing. The amended microcosms hosted more diverse bacterial communities compared to the intrinsic fracture zone community and the control treatment without amendments. The majority of the bacterial populations enriched with acetate belonged to clostridial hydrogenotrophic thiosulfate reducers and Alphaproteobacteria affiliating with groups earlier found from subsurface and groundwater environments. We detected a slight increase in the number of sulfate reducers after the 68 days of incubation. The microbial community changed significantly during the experiment, but increase in specifically acetate-cycling microbial groups was not observed.
Original languageEnglish
Article number232
Pages (from-to)232
JournalFrontiers in Microbiology
Volume8
Issue numberFeb
DOIs
Publication statusPublished - 20 Feb 2017
MoE publication typeA1 Journal article-refereed

Fingerprint

Acetates
Carbon
Electrons
Sulfates
Groundwater
Alphaproteobacteria
Thiosulfates
Atmosphere
rRNA Genes
Hydrogen
Population

Keywords

  • acetate
  • autotrophy
  • competition
  • crystalline terrestrial bedrock
  • deep biosphere
  • enrichment culture
  • heterotrophy
  • sulfate reducing bacteria

Cite this

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abstract = "Acetate plays a key role as electron donor and acceptor and serves as carbon source in oligotrophic deep subsurface environments. It can be produced from inorganic carbon by acetogenic microbes or through breakdown of more complex organic matter. Acetate is an important molecule for sulfate reducers that are substantially present in several deep bedrock environments. Aceticlastic methanogens use acetate as an electron donor and/or a carbon source. The goal of this study was to shed light on carbon cycling and competition in microbial communities in fracture fluids of Finnish crystalline bedrock groundwater system. Fracture fluid was anaerobically collected from a fracture zone at 967 m depth of the Outokumpu Deep Drill Hole and amended with acetate, acetate + sulfate, sulfate only or left unamended as a control and incubated up to 68 days. The headspace atmosphere of microcosms consisted of 80{\%} hydrogen and 20{\%} CO2. We studied the changes in the microbial communities with community fingerprinting technique as well as high-throughput 16S rRNA gene amplicon sequencing. The amended microcosms hosted more diverse bacterial communities compared to the intrinsic fracture zone community and the control treatment without amendments. The majority of the bacterial populations enriched with acetate belonged to clostridial hydrogenotrophic thiosulfate reducers and Alphaproteobacteria affiliating with groups earlier found from subsurface and groundwater environments. We detected a slight increase in the number of sulfate reducers after the 68 days of incubation. The microbial community changed significantly during the experiment, but increase in specifically acetate-cycling microbial groups was not observed.",
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Response of deep subsurface microbial community to different carbon sources and electron acceptors during ~2 months incubation in microcosms. / Purkamo, Lotta; Bomberg, Malin; Nyyssönen, Mari; Ahonen, Lasse; Kukkonen, Ilmo; Itävaara, Merja.

In: Frontiers in Microbiology, Vol. 8, No. Feb, 232, 20.02.2017, p. 232.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Response of deep subsurface microbial community to different carbon sources and electron acceptors during ~2 months incubation in microcosms

AU - Purkamo, Lotta

AU - Bomberg, Malin

AU - Nyyssönen, Mari

AU - Ahonen, Lasse

AU - Kukkonen, Ilmo

AU - Itävaara, Merja

PY - 2017/2/20

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N2 - Acetate plays a key role as electron donor and acceptor and serves as carbon source in oligotrophic deep subsurface environments. It can be produced from inorganic carbon by acetogenic microbes or through breakdown of more complex organic matter. Acetate is an important molecule for sulfate reducers that are substantially present in several deep bedrock environments. Aceticlastic methanogens use acetate as an electron donor and/or a carbon source. The goal of this study was to shed light on carbon cycling and competition in microbial communities in fracture fluids of Finnish crystalline bedrock groundwater system. Fracture fluid was anaerobically collected from a fracture zone at 967 m depth of the Outokumpu Deep Drill Hole and amended with acetate, acetate + sulfate, sulfate only or left unamended as a control and incubated up to 68 days. The headspace atmosphere of microcosms consisted of 80% hydrogen and 20% CO2. We studied the changes in the microbial communities with community fingerprinting technique as well as high-throughput 16S rRNA gene amplicon sequencing. The amended microcosms hosted more diverse bacterial communities compared to the intrinsic fracture zone community and the control treatment without amendments. The majority of the bacterial populations enriched with acetate belonged to clostridial hydrogenotrophic thiosulfate reducers and Alphaproteobacteria affiliating with groups earlier found from subsurface and groundwater environments. We detected a slight increase in the number of sulfate reducers after the 68 days of incubation. The microbial community changed significantly during the experiment, but increase in specifically acetate-cycling microbial groups was not observed.

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KW - acetate

KW - autotrophy

KW - competition

KW - crystalline terrestrial bedrock

KW - deep biosphere

KW - enrichment culture

KW - heterotrophy

KW - sulfate reducing bacteria

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U2 - 10.3389/fmicb.2017.00232

DO - 10.3389/fmicb.2017.00232

M3 - Article

VL - 8

SP - 232

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

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ER -