Responses of Saccharomyces cerevisiae to changing oxygen level

Marilyn Wiebe, Eija Rintala, Merja Toivari, Anu Tamminen, Laura Salusjärvi, Paula Jouhten, Anne Huuskonen, Helena Simolin, Juha Kokkonen, Jari Kiuru, Hannu Maaheimo, Laura Ruohonen, Merja Penttilä

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

We studied the responses of Saccharomyces cerevisiae in varying oxygen concentrations on metabolite, transcriptome and proteome level. The S. cerevisiae strain CEN.PK113-1A was grown in D-glucose-limited chemostat cultures in five different oxygen concentrations: 0%, 0.5%, 1%, 2.8% and 20.9% O2 of the inlet gas. After reaching a steady state the oxygen was shut off from the cultures of 1% and 20.9% O2 of the inlet gas and the metabolism followed for approximately 70 hours. From both steady state and transient culture samples 17 metabolites of central carbon metabolism were analyzed. Changes in the transcriptome were monitored with Affymetrix-arrays and with the VTT-TRAC method. In addition, alterations in the proteome were analyzed with 2-D gels. The concentrations of metabolites of tricarboxylic acid cycle (TCA) and most glycolytic metabolites were, in general, higher in anaerobic or low aeration cultures (0%, 0.5% and 1% O2 of the inlet gas) compared with more aerobic conditions (2.8% and 20% O2 of the inlet gas). Similarly, after oxygen shut off from the cultures of 1% or 20.9% O2 of the inlet gas the level of TCA metabolites and those of upper glycolysis increased. On the transcriptome level the responses varied more, some genes being upregulated and others down regulated in steady state conditions and showing varying responses during transient conditions. As evaluated from both the metabolite and transcription data approximately 30 h was required to reach a new steady state of anaerobiosis.
Original languageEnglish
Title of host publication3rd European Federation of Biotechnology Conference
Subtitle of host publicationPhysiology of Yeasts and Filamentous Fungi PYFF3
Place of PublicationEspoo
PublisherVTT Technical Research Centre of Finland
Pages114
ISBN (Electronic)978-951-38-6314-2
ISBN (Print)978-951-38-6313-5
Publication statusPublished - 2007
Event3rd European Federation of Biotechnology Conference : Physiology of Yeasts and Filamentous Fungi - Helsinki, Finland
Duration: 13 Jun 200716 Jun 2007

Publication series

SeriesVTT Symposium
Number245
ISSN0357-9387

Conference

Conference3rd European Federation of Biotechnology Conference
Abbreviated titlePYFF3
CountryFinland
CityHelsinki
Period13/06/0716/06/07

Fingerprint

Saccharomyces cerevisiae
metabolites
oxygen
gases
transcriptome
tricarboxylic acid cycle
proteome
metabolism
anaerobiosis
aerobic conditions
glycolysis
aeration
transcription (genetics)
gels
glucose
carbon
genes
sampling

Cite this

Wiebe, M., Rintala, E., Toivari, M., Tamminen, A., Salusjärvi, L., Jouhten, P., ... Penttilä, M. (2007). Responses of Saccharomyces cerevisiae to changing oxygen level. In 3rd European Federation of Biotechnology Conference: Physiology of Yeasts and Filamentous Fungi PYFF3 (pp. 114). [P60] Espoo: VTT Technical Research Centre of Finland. VTT Symposium, No. 245
Wiebe, Marilyn ; Rintala, Eija ; Toivari, Merja ; Tamminen, Anu ; Salusjärvi, Laura ; Jouhten, Paula ; Huuskonen, Anne ; Simolin, Helena ; Kokkonen, Juha ; Kiuru, Jari ; Maaheimo, Hannu ; Ruohonen, Laura ; Penttilä, Merja. / Responses of Saccharomyces cerevisiae to changing oxygen level. 3rd European Federation of Biotechnology Conference: Physiology of Yeasts and Filamentous Fungi PYFF3. Espoo : VTT Technical Research Centre of Finland, 2007. pp. 114 (VTT Symposium; No. 245).
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Wiebe, M, Rintala, E, Toivari, M, Tamminen, A, Salusjärvi, L, Jouhten, P, Huuskonen, A, Simolin, H, Kokkonen, J, Kiuru, J, Maaheimo, H, Ruohonen, L & Penttilä, M 2007, Responses of Saccharomyces cerevisiae to changing oxygen level. in 3rd European Federation of Biotechnology Conference: Physiology of Yeasts and Filamentous Fungi PYFF3., P60, VTT Technical Research Centre of Finland, Espoo, VTT Symposium, no. 245, pp. 114, 3rd European Federation of Biotechnology Conference , Helsinki, Finland, 13/06/07.

Responses of Saccharomyces cerevisiae to changing oxygen level. / Wiebe, Marilyn; Rintala, Eija; Toivari, Merja; Tamminen, Anu; Salusjärvi, Laura; Jouhten, Paula; Huuskonen, Anne; Simolin, Helena; Kokkonen, Juha; Kiuru, Jari; Maaheimo, Hannu; Ruohonen, Laura; Penttilä, Merja.

3rd European Federation of Biotechnology Conference: Physiology of Yeasts and Filamentous Fungi PYFF3. Espoo : VTT Technical Research Centre of Finland, 2007. p. 114 P60 (VTT Symposium; No. 245).

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

TY - CHAP

T1 - Responses of Saccharomyces cerevisiae to changing oxygen level

AU - Wiebe, Marilyn

AU - Rintala, Eija

AU - Toivari, Merja

AU - Tamminen, Anu

AU - Salusjärvi, Laura

AU - Jouhten, Paula

AU - Huuskonen, Anne

AU - Simolin, Helena

AU - Kokkonen, Juha

AU - Kiuru, Jari

AU - Maaheimo, Hannu

AU - Ruohonen, Laura

AU - Penttilä, Merja

PY - 2007

Y1 - 2007

N2 - We studied the responses of Saccharomyces cerevisiae in varying oxygen concentrations on metabolite, transcriptome and proteome level. The S. cerevisiae strain CEN.PK113-1A was grown in D-glucose-limited chemostat cultures in five different oxygen concentrations: 0%, 0.5%, 1%, 2.8% and 20.9% O2 of the inlet gas. After reaching a steady state the oxygen was shut off from the cultures of 1% and 20.9% O2 of the inlet gas and the metabolism followed for approximately 70 hours. From both steady state and transient culture samples 17 metabolites of central carbon metabolism were analyzed. Changes in the transcriptome were monitored with Affymetrix-arrays and with the VTT-TRAC method. In addition, alterations in the proteome were analyzed with 2-D gels. The concentrations of metabolites of tricarboxylic acid cycle (TCA) and most glycolytic metabolites were, in general, higher in anaerobic or low aeration cultures (0%, 0.5% and 1% O2 of the inlet gas) compared with more aerobic conditions (2.8% and 20% O2 of the inlet gas). Similarly, after oxygen shut off from the cultures of 1% or 20.9% O2 of the inlet gas the level of TCA metabolites and those of upper glycolysis increased. On the transcriptome level the responses varied more, some genes being upregulated and others down regulated in steady state conditions and showing varying responses during transient conditions. As evaluated from both the metabolite and transcription data approximately 30 h was required to reach a new steady state of anaerobiosis.

AB - We studied the responses of Saccharomyces cerevisiae in varying oxygen concentrations on metabolite, transcriptome and proteome level. The S. cerevisiae strain CEN.PK113-1A was grown in D-glucose-limited chemostat cultures in five different oxygen concentrations: 0%, 0.5%, 1%, 2.8% and 20.9% O2 of the inlet gas. After reaching a steady state the oxygen was shut off from the cultures of 1% and 20.9% O2 of the inlet gas and the metabolism followed for approximately 70 hours. From both steady state and transient culture samples 17 metabolites of central carbon metabolism were analyzed. Changes in the transcriptome were monitored with Affymetrix-arrays and with the VTT-TRAC method. In addition, alterations in the proteome were analyzed with 2-D gels. The concentrations of metabolites of tricarboxylic acid cycle (TCA) and most glycolytic metabolites were, in general, higher in anaerobic or low aeration cultures (0%, 0.5% and 1% O2 of the inlet gas) compared with more aerobic conditions (2.8% and 20% O2 of the inlet gas). Similarly, after oxygen shut off from the cultures of 1% or 20.9% O2 of the inlet gas the level of TCA metabolites and those of upper glycolysis increased. On the transcriptome level the responses varied more, some genes being upregulated and others down regulated in steady state conditions and showing varying responses during transient conditions. As evaluated from both the metabolite and transcription data approximately 30 h was required to reach a new steady state of anaerobiosis.

M3 - Conference abstract in proceedings

SN - 978-951-38-6313-5

T3 - VTT Symposium

SP - 114

BT - 3rd European Federation of Biotechnology Conference

PB - VTT Technical Research Centre of Finland

CY - Espoo

ER -

Wiebe M, Rintala E, Toivari M, Tamminen A, Salusjärvi L, Jouhten P et al. Responses of Saccharomyces cerevisiae to changing oxygen level. In 3rd European Federation of Biotechnology Conference: Physiology of Yeasts and Filamentous Fungi PYFF3. Espoo: VTT Technical Research Centre of Finland. 2007. p. 114. P60. (VTT Symposium; No. 245).