Retention of lysozyme activity by physical immobilization in nanocellulose aerogels and antibacterial effects

Khan Uddin, Hannes Orelma, Pezhman Mohammadi, Maryam Borghei, Janne Laine, Markus Linder, Orlando Rojas

    Research output: Contribution to journalArticleScientificpeer-review

    6 Citations (Scopus)

    Abstract

    Aerogels prepared from aqueous dispersions of anionic and cationic cellulose nanofibrils (CNFs) were investigated as solid supports for enzymes and silver nanoparticles and to elicit a sustained antibacterial effect. The imparted stabilization in dry conditions was studied with aerogels that were cast after mixing the enzymes with CNFs followed by dehydration (freeze-drying). The activity of lysozyme immobilized in the given CNF system was analyzed upon storage in liquid and air media. In contrast with aqueous solutions of free, unbound enzyme, which lost activity after the first day, the enzyme immobilized physically in unmodified and cationic CNF presented better stability (activity for a longer time). However, the enzyme activity was reduced in the case of anionic CNF, which was prepared by TEMPO-mediated oxidation (TO-CNF). Both humidity and temperature reduced the stability of the enzyme immobilized in the respective CNF aerogel. The antibacterial activity of CNF aerogels carrying lysozyme was also tested against gram-negative and gram-positive bacteria. The results were compared with those obtained from CNF systems loaded with silver nanoparticles (AgNP) after in situ synthesis via UV reduction. Storage in cold or dry conditions preserved the activity and antibacterial performance of enzyme-loaded CNF aerogels. As expected, the lysozyme-containing aerogels showed lower inhibition than the AgNP-containing aerogel. In this latter case, the antibacterial activity depended on the concentration and size of the nanoparticles. Compared to unmodified CNF and TO-CNF, the aerogels prepared with cationic CNF, loaded with either lysozyme or AgNPs, showed remarkably better antibacterial activity. Similar experiments were conducted with horseradish peroxidase, which confirmed, to different degrees, the observations derived from the lysozyme systems. Overall, the results indicate that non-toxic and biodegradable CNF is a suitable support for bio-active materials and is effective in protecting and retaining enzymatic and antibacterial activities.
    Original languageEnglish
    Pages (from-to)2837-2848
    Number of pages12
    JournalCellulose
    Volume24
    Issue number7
    DOIs
    Publication statusPublished - 1 Jul 2017
    MoE publication typeA1 Journal article-refereed

    Fingerprint

    Aerogels
    Muramidase
    Cellulose
    Enzymes
    Immobilized Enzymes
    Nanoparticles
    Silver
    Enzyme inhibition
    Enzyme activity
    Horseradish Peroxidase
    Dehydration
    Dispersions
    Catalyst supports
    Catalyst activity
    Atmospheric humidity
    Bacteria
    Drying

    Keywords

    • antibacterial aerogel
    • cellulose nanofibrils (CNF)
    • enzyme stability
    • lysozyme
    • silver nanoparticles

    Cite this

    Uddin, Khan ; Orelma, Hannes ; Mohammadi, Pezhman ; Borghei, Maryam ; Laine, Janne ; Linder, Markus ; Rojas, Orlando. / Retention of lysozyme activity by physical immobilization in nanocellulose aerogels and antibacterial effects. In: Cellulose. 2017 ; Vol. 24, No. 7. pp. 2837-2848.
    @article{4741cca784814d9d9471b31a420423bc,
    title = "Retention of lysozyme activity by physical immobilization in nanocellulose aerogels and antibacterial effects",
    abstract = "Aerogels prepared from aqueous dispersions of anionic and cationic cellulose nanofibrils (CNFs) were investigated as solid supports for enzymes and silver nanoparticles and to elicit a sustained antibacterial effect. The imparted stabilization in dry conditions was studied with aerogels that were cast after mixing the enzymes with CNFs followed by dehydration (freeze-drying). The activity of lysozyme immobilized in the given CNF system was analyzed upon storage in liquid and air media. In contrast with aqueous solutions of free, unbound enzyme, which lost activity after the first day, the enzyme immobilized physically in unmodified and cationic CNF presented better stability (activity for a longer time). However, the enzyme activity was reduced in the case of anionic CNF, which was prepared by TEMPO-mediated oxidation (TO-CNF). Both humidity and temperature reduced the stability of the enzyme immobilized in the respective CNF aerogel. The antibacterial activity of CNF aerogels carrying lysozyme was also tested against gram-negative and gram-positive bacteria. The results were compared with those obtained from CNF systems loaded with silver nanoparticles (AgNP) after in situ synthesis via UV reduction. Storage in cold or dry conditions preserved the activity and antibacterial performance of enzyme-loaded CNF aerogels. As expected, the lysozyme-containing aerogels showed lower inhibition than the AgNP-containing aerogel. In this latter case, the antibacterial activity depended on the concentration and size of the nanoparticles. Compared to unmodified CNF and TO-CNF, the aerogels prepared with cationic CNF, loaded with either lysozyme or AgNPs, showed remarkably better antibacterial activity. Similar experiments were conducted with horseradish peroxidase, which confirmed, to different degrees, the observations derived from the lysozyme systems. Overall, the results indicate that non-toxic and biodegradable CNF is a suitable support for bio-active materials and is effective in protecting and retaining enzymatic and antibacterial activities.",
    keywords = "antibacterial aerogel, cellulose nanofibrils (CNF), enzyme stability, lysozyme, silver nanoparticles",
    author = "Khan Uddin and Hannes Orelma and Pezhman Mohammadi and Maryam Borghei and Janne Laine and Markus Linder and Orlando Rojas",
    year = "2017",
    month = "7",
    day = "1",
    doi = "10.1007/s10570-017-1311-0",
    language = "English",
    volume = "24",
    pages = "2837--2848",
    journal = "Cellulose",
    issn = "0969-0239",
    publisher = "Springer",
    number = "7",

    }

    Retention of lysozyme activity by physical immobilization in nanocellulose aerogels and antibacterial effects. / Uddin, Khan; Orelma, Hannes; Mohammadi, Pezhman; Borghei, Maryam; Laine, Janne; Linder, Markus; Rojas, Orlando.

    In: Cellulose, Vol. 24, No. 7, 01.07.2017, p. 2837-2848.

    Research output: Contribution to journalArticleScientificpeer-review

    TY - JOUR

    T1 - Retention of lysozyme activity by physical immobilization in nanocellulose aerogels and antibacterial effects

    AU - Uddin, Khan

    AU - Orelma, Hannes

    AU - Mohammadi, Pezhman

    AU - Borghei, Maryam

    AU - Laine, Janne

    AU - Linder, Markus

    AU - Rojas, Orlando

    PY - 2017/7/1

    Y1 - 2017/7/1

    N2 - Aerogels prepared from aqueous dispersions of anionic and cationic cellulose nanofibrils (CNFs) were investigated as solid supports for enzymes and silver nanoparticles and to elicit a sustained antibacterial effect. The imparted stabilization in dry conditions was studied with aerogels that were cast after mixing the enzymes with CNFs followed by dehydration (freeze-drying). The activity of lysozyme immobilized in the given CNF system was analyzed upon storage in liquid and air media. In contrast with aqueous solutions of free, unbound enzyme, which lost activity after the first day, the enzyme immobilized physically in unmodified and cationic CNF presented better stability (activity for a longer time). However, the enzyme activity was reduced in the case of anionic CNF, which was prepared by TEMPO-mediated oxidation (TO-CNF). Both humidity and temperature reduced the stability of the enzyme immobilized in the respective CNF aerogel. The antibacterial activity of CNF aerogels carrying lysozyme was also tested against gram-negative and gram-positive bacteria. The results were compared with those obtained from CNF systems loaded with silver nanoparticles (AgNP) after in situ synthesis via UV reduction. Storage in cold or dry conditions preserved the activity and antibacterial performance of enzyme-loaded CNF aerogels. As expected, the lysozyme-containing aerogels showed lower inhibition than the AgNP-containing aerogel. In this latter case, the antibacterial activity depended on the concentration and size of the nanoparticles. Compared to unmodified CNF and TO-CNF, the aerogels prepared with cationic CNF, loaded with either lysozyme or AgNPs, showed remarkably better antibacterial activity. Similar experiments were conducted with horseradish peroxidase, which confirmed, to different degrees, the observations derived from the lysozyme systems. Overall, the results indicate that non-toxic and biodegradable CNF is a suitable support for bio-active materials and is effective in protecting and retaining enzymatic and antibacterial activities.

    AB - Aerogels prepared from aqueous dispersions of anionic and cationic cellulose nanofibrils (CNFs) were investigated as solid supports for enzymes and silver nanoparticles and to elicit a sustained antibacterial effect. The imparted stabilization in dry conditions was studied with aerogels that were cast after mixing the enzymes with CNFs followed by dehydration (freeze-drying). The activity of lysozyme immobilized in the given CNF system was analyzed upon storage in liquid and air media. In contrast with aqueous solutions of free, unbound enzyme, which lost activity after the first day, the enzyme immobilized physically in unmodified and cationic CNF presented better stability (activity for a longer time). However, the enzyme activity was reduced in the case of anionic CNF, which was prepared by TEMPO-mediated oxidation (TO-CNF). Both humidity and temperature reduced the stability of the enzyme immobilized in the respective CNF aerogel. The antibacterial activity of CNF aerogels carrying lysozyme was also tested against gram-negative and gram-positive bacteria. The results were compared with those obtained from CNF systems loaded with silver nanoparticles (AgNP) after in situ synthesis via UV reduction. Storage in cold or dry conditions preserved the activity and antibacterial performance of enzyme-loaded CNF aerogels. As expected, the lysozyme-containing aerogels showed lower inhibition than the AgNP-containing aerogel. In this latter case, the antibacterial activity depended on the concentration and size of the nanoparticles. Compared to unmodified CNF and TO-CNF, the aerogels prepared with cationic CNF, loaded with either lysozyme or AgNPs, showed remarkably better antibacterial activity. Similar experiments were conducted with horseradish peroxidase, which confirmed, to different degrees, the observations derived from the lysozyme systems. Overall, the results indicate that non-toxic and biodegradable CNF is a suitable support for bio-active materials and is effective in protecting and retaining enzymatic and antibacterial activities.

    KW - antibacterial aerogel

    KW - cellulose nanofibrils (CNF)

    KW - enzyme stability

    KW - lysozyme

    KW - silver nanoparticles

    UR - http://www.scopus.com/inward/record.url?scp=85018791478&partnerID=8YFLogxK

    U2 - 10.1007/s10570-017-1311-0

    DO - 10.1007/s10570-017-1311-0

    M3 - Article

    VL - 24

    SP - 2837

    EP - 2848

    JO - Cellulose

    JF - Cellulose

    SN - 0969-0239

    IS - 7

    ER -