Role of lignin activation in laccase-catalysed functionalisation of lignin-containing materials

Anna Suurnäkki, Hannu Mikkonen, Marco Orlandi, Carmen Canevali, Terhi Saarinen, Monica Österberg, Janne Laine, Liisa Viikari

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientific

Abstract

Lignocellulosic materials such as wood pulps and non-wood fibres can be modified by chemo-enzymatic functionalisation method [WO2005/061790]. This method enables the further development of native properties and even the creation of completely novel properties to lignin-containing materials. The method is based on the ability of oxidative enzymes, especially laccases, to catalyze the formation of phenoxy radicals which can thereafter be used as reactive site to further bonding of selected functional chemical groups to lignin-containing materials. Chemo-enzymatic modification is thus composed of two steps, activation and bonding. Understanding of the role of each step and the correlation between these steps is important regarding the result of modification by chemo-enzymatic functionalisation. The role of lignin activation in laccase-catalysed functionalisation of lignin-containing materials has been studied by EPR measurements and by bonding model compounds to differently activated lignin-containing wood pulps. The effect of enzyme type and its ability to bond to lignin substrate during activation step on subsequent bonding of functional chemical has been elucidated with the help of dynamic QCM-D measurements. The factors affecting the activation and functionalisation as well the correlation between the activation and bonding step will be discussed based on the results obtained.
Original languageEnglish
Title of host publicationLignobiotech One Symposium
Subtitle of host publication1st Symposium on Biotechnology Applied to Lignocelluloses : March 28th - April 1st 2010, Reims, France
PublisherPresses universitaires de Reims
Publication statusPublished - 2010
MoE publication typeNot Eligible
Event1st Symposium on Biotechnology Applied to Lignocelluloses, LignoBiotech One - Reims, France
Duration: 28 Mar 20101 Apr 2010

Conference

Conference1st Symposium on Biotechnology Applied to Lignocelluloses, LignoBiotech One
Abbreviated titleLignoBiotech One Symposium
CountryFrance
CityReims
Period28/03/101/04/10

Fingerprint

Laccase
Lignin
Chemical activation
Pulp
Wood
Enzymes
Paramagnetic resonance
Fibers
Substrates

Cite this

Suurnäkki, A., Mikkonen, H., Orlandi, M., Canevali, C., Saarinen, T., Österberg, M., ... Viikari, L. (2010). Role of lignin activation in laccase-catalysed functionalisation of lignin-containing materials. In Lignobiotech One Symposium: 1st Symposium on Biotechnology Applied to Lignocelluloses : March 28th - April 1st 2010, Reims, France Presses universitaires de Reims.
Suurnäkki, Anna ; Mikkonen, Hannu ; Orlandi, Marco ; Canevali, Carmen ; Saarinen, Terhi ; Österberg, Monica ; Laine, Janne ; Viikari, Liisa. / Role of lignin activation in laccase-catalysed functionalisation of lignin-containing materials. Lignobiotech One Symposium: 1st Symposium on Biotechnology Applied to Lignocelluloses : March 28th - April 1st 2010, Reims, France. Presses universitaires de Reims, 2010.
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title = "Role of lignin activation in laccase-catalysed functionalisation of lignin-containing materials",
abstract = "Lignocellulosic materials such as wood pulps and non-wood fibres can be modified by chemo-enzymatic functionalisation method [WO2005/061790]. This method enables the further development of native properties and even the creation of completely novel properties to lignin-containing materials. The method is based on the ability of oxidative enzymes, especially laccases, to catalyze the formation of phenoxy radicals which can thereafter be used as reactive site to further bonding of selected functional chemical groups to lignin-containing materials. Chemo-enzymatic modification is thus composed of two steps, activation and bonding. Understanding of the role of each step and the correlation between these steps is important regarding the result of modification by chemo-enzymatic functionalisation. The role of lignin activation in laccase-catalysed functionalisation of lignin-containing materials has been studied by EPR measurements and by bonding model compounds to differently activated lignin-containing wood pulps. The effect of enzyme type and its ability to bond to lignin substrate during activation step on subsequent bonding of functional chemical has been elucidated with the help of dynamic QCM-D measurements. The factors affecting the activation and functionalisation as well the correlation between the activation and bonding step will be discussed based on the results obtained.",
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Suurnäkki, A, Mikkonen, H, Orlandi, M, Canevali, C, Saarinen, T, Österberg, M, Laine, J & Viikari, L 2010, Role of lignin activation in laccase-catalysed functionalisation of lignin-containing materials. in Lignobiotech One Symposium: 1st Symposium on Biotechnology Applied to Lignocelluloses : March 28th - April 1st 2010, Reims, France. Presses universitaires de Reims, 1st Symposium on Biotechnology Applied to Lignocelluloses, LignoBiotech One, Reims, France, 28/03/10.

Role of lignin activation in laccase-catalysed functionalisation of lignin-containing materials. / Suurnäkki, Anna; Mikkonen, Hannu; Orlandi, Marco; Canevali, Carmen; Saarinen, Terhi; Österberg, Monica; Laine, Janne; Viikari, Liisa.

Lignobiotech One Symposium: 1st Symposium on Biotechnology Applied to Lignocelluloses : March 28th - April 1st 2010, Reims, France. Presses universitaires de Reims, 2010.

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientific

TY - GEN

T1 - Role of lignin activation in laccase-catalysed functionalisation of lignin-containing materials

AU - Suurnäkki, Anna

AU - Mikkonen, Hannu

AU - Orlandi, Marco

AU - Canevali, Carmen

AU - Saarinen, Terhi

AU - Österberg, Monica

AU - Laine, Janne

AU - Viikari, Liisa

PY - 2010

Y1 - 2010

N2 - Lignocellulosic materials such as wood pulps and non-wood fibres can be modified by chemo-enzymatic functionalisation method [WO2005/061790]. This method enables the further development of native properties and even the creation of completely novel properties to lignin-containing materials. The method is based on the ability of oxidative enzymes, especially laccases, to catalyze the formation of phenoxy radicals which can thereafter be used as reactive site to further bonding of selected functional chemical groups to lignin-containing materials. Chemo-enzymatic modification is thus composed of two steps, activation and bonding. Understanding of the role of each step and the correlation between these steps is important regarding the result of modification by chemo-enzymatic functionalisation. The role of lignin activation in laccase-catalysed functionalisation of lignin-containing materials has been studied by EPR measurements and by bonding model compounds to differently activated lignin-containing wood pulps. The effect of enzyme type and its ability to bond to lignin substrate during activation step on subsequent bonding of functional chemical has been elucidated with the help of dynamic QCM-D measurements. The factors affecting the activation and functionalisation as well the correlation between the activation and bonding step will be discussed based on the results obtained.

AB - Lignocellulosic materials such as wood pulps and non-wood fibres can be modified by chemo-enzymatic functionalisation method [WO2005/061790]. This method enables the further development of native properties and even the creation of completely novel properties to lignin-containing materials. The method is based on the ability of oxidative enzymes, especially laccases, to catalyze the formation of phenoxy radicals which can thereafter be used as reactive site to further bonding of selected functional chemical groups to lignin-containing materials. Chemo-enzymatic modification is thus composed of two steps, activation and bonding. Understanding of the role of each step and the correlation between these steps is important regarding the result of modification by chemo-enzymatic functionalisation. The role of lignin activation in laccase-catalysed functionalisation of lignin-containing materials has been studied by EPR measurements and by bonding model compounds to differently activated lignin-containing wood pulps. The effect of enzyme type and its ability to bond to lignin substrate during activation step on subsequent bonding of functional chemical has been elucidated with the help of dynamic QCM-D measurements. The factors affecting the activation and functionalisation as well the correlation between the activation and bonding step will be discussed based on the results obtained.

M3 - Conference article in proceedings

BT - Lignobiotech One Symposium

PB - Presses universitaires de Reims

ER -

Suurnäkki A, Mikkonen H, Orlandi M, Canevali C, Saarinen T, Österberg M et al. Role of lignin activation in laccase-catalysed functionalisation of lignin-containing materials. In Lignobiotech One Symposium: 1st Symposium on Biotechnology Applied to Lignocelluloses : March 28th - April 1st 2010, Reims, France. Presses universitaires de Reims. 2010