Role of oxidative enzymatic treatments on enzymatic hydrolysis of softwood

Hetti Palonen, Liisa Viikari (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

102 Citations (Scopus)

Abstract

The impact of oxidative modification and partial removal of lignin by laccase–mediator treatments on the enzymatic hydrolysis of steam‐pretreated softwood (SPS) was evaluated. Two mediators, N‐hydroxy‐N‐phenylacetamide (NHA) and its acetylated precursor, were oxidized by the laccase from Trametes hirsuta, and their effects on the activity of cellulolytic enzymes and on the hydrolysis yield of SPS were examined. Both simultaneous and sequential combinations of laccase–mediator treatments with commercial cellulases increased the sugar yield in the enzymatic hydrolysis of SPS. The maximal increase was 21% when a sequential treatment was applied. Laccase treatment alone was also shown to improve hydrolysis. NHA oxidized by laccase inhibited significantly the cellulases of Trichoderma reesei, but the presence of the solid substrate protected the activities against oxidative inactivation. Surface analysis of the lignocellulosic substrate before and after the laccase and cellulase treatments revealed an enrichment of lignin and an increase of carboxylic groups on the surface of the hydrolysis residue.
Original languageEnglish
Pages (from-to)550-557
JournalBiotechnology and Bioengineering
Volume86
Issue number5
DOIs
Publication statusPublished - 2004
MoE publication typeA1 Journal article-refereed

Fingerprint

Laccase
Enzymatic hydrolysis
Softwoods
Hydrolysis
Lignin
Cellulases
Surface analysis
Substrates
Sugars
Cellulase
Trametes
Enzymes
Trichoderma

Keywords

  • cellulose hydrolysis
  • role of lignin
  • surface modification

Cite this

Palonen, Hetti ; Viikari, Liisa. / Role of oxidative enzymatic treatments on enzymatic hydrolysis of softwood. In: Biotechnology and Bioengineering. 2004 ; Vol. 86, No. 5. pp. 550-557.
@article{d6f2d1ee78194486ba610b59f6d1f784,
title = "Role of oxidative enzymatic treatments on enzymatic hydrolysis of softwood",
abstract = "The impact of oxidative modification and partial removal of lignin by laccase–mediator treatments on the enzymatic hydrolysis of steam‐pretreated softwood (SPS) was evaluated. Two mediators, N‐hydroxy‐N‐phenylacetamide (NHA) and its acetylated precursor, were oxidized by the laccase from Trametes hirsuta, and their effects on the activity of cellulolytic enzymes and on the hydrolysis yield of SPS were examined. Both simultaneous and sequential combinations of laccase–mediator treatments with commercial cellulases increased the sugar yield in the enzymatic hydrolysis of SPS. The maximal increase was 21{\%} when a sequential treatment was applied. Laccase treatment alone was also shown to improve hydrolysis. NHA oxidized by laccase inhibited significantly the cellulases of Trichoderma reesei, but the presence of the solid substrate protected the activities against oxidative inactivation. Surface analysis of the lignocellulosic substrate before and after the laccase and cellulase treatments revealed an enrichment of lignin and an increase of carboxylic groups on the surface of the hydrolysis residue.",
keywords = "cellulose hydrolysis, role of lignin, surface modification",
author = "Hetti Palonen and Liisa Viikari",
year = "2004",
doi = "10.1002/bit.20135",
language = "English",
volume = "86",
pages = "550--557",
journal = "Biotechnology and Bioengineering",
issn = "0006-3592",
publisher = "Wiley",
number = "5",

}

Role of oxidative enzymatic treatments on enzymatic hydrolysis of softwood. / Palonen, Hetti; Viikari, Liisa (Corresponding Author).

In: Biotechnology and Bioengineering, Vol. 86, No. 5, 2004, p. 550-557.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Role of oxidative enzymatic treatments on enzymatic hydrolysis of softwood

AU - Palonen, Hetti

AU - Viikari, Liisa

PY - 2004

Y1 - 2004

N2 - The impact of oxidative modification and partial removal of lignin by laccase–mediator treatments on the enzymatic hydrolysis of steam‐pretreated softwood (SPS) was evaluated. Two mediators, N‐hydroxy‐N‐phenylacetamide (NHA) and its acetylated precursor, were oxidized by the laccase from Trametes hirsuta, and their effects on the activity of cellulolytic enzymes and on the hydrolysis yield of SPS were examined. Both simultaneous and sequential combinations of laccase–mediator treatments with commercial cellulases increased the sugar yield in the enzymatic hydrolysis of SPS. The maximal increase was 21% when a sequential treatment was applied. Laccase treatment alone was also shown to improve hydrolysis. NHA oxidized by laccase inhibited significantly the cellulases of Trichoderma reesei, but the presence of the solid substrate protected the activities against oxidative inactivation. Surface analysis of the lignocellulosic substrate before and after the laccase and cellulase treatments revealed an enrichment of lignin and an increase of carboxylic groups on the surface of the hydrolysis residue.

AB - The impact of oxidative modification and partial removal of lignin by laccase–mediator treatments on the enzymatic hydrolysis of steam‐pretreated softwood (SPS) was evaluated. Two mediators, N‐hydroxy‐N‐phenylacetamide (NHA) and its acetylated precursor, were oxidized by the laccase from Trametes hirsuta, and their effects on the activity of cellulolytic enzymes and on the hydrolysis yield of SPS were examined. Both simultaneous and sequential combinations of laccase–mediator treatments with commercial cellulases increased the sugar yield in the enzymatic hydrolysis of SPS. The maximal increase was 21% when a sequential treatment was applied. Laccase treatment alone was also shown to improve hydrolysis. NHA oxidized by laccase inhibited significantly the cellulases of Trichoderma reesei, but the presence of the solid substrate protected the activities against oxidative inactivation. Surface analysis of the lignocellulosic substrate before and after the laccase and cellulase treatments revealed an enrichment of lignin and an increase of carboxylic groups on the surface of the hydrolysis residue.

KW - cellulose hydrolysis

KW - role of lignin

KW - surface modification

U2 - 10.1002/bit.20135

DO - 10.1002/bit.20135

M3 - Article

VL - 86

SP - 550

EP - 557

JO - Biotechnology and Bioengineering

JF - Biotechnology and Bioengineering

SN - 0006-3592

IS - 5

ER -