Roll-to-roll fabricated self-filling polydimethylsiloxane diagnostic platforms for multiplexed pathogen nucleic acid detection

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review


Polydimethylsiloxane (PDMS) is a commonly used material for prototyping microfluidics due to its biocompatibility, transparency, elasticity, low auto-fluorescence, and low cost in comparison to similar materials. PDMS-based devices provide alternatives for conventional diagnostic materials, such as polymerase chain reaction (PCR) tubes, that are limited in supply due to high demand in pandemics or similar situations. Roll-to-roll (R2R) production enables mass production of PDMS-based devices for point-of-care (PoC) diagnostics of pathogen nucleic acids. Mass manufacturing and end user points-of-view must be considered when designing a new assay. Isothermal nucleic acid amplification techniques enable less complex heating solutions. Self-filling of the device without external equipment, such as vacuum pumps, is also necessary for user-friendly and portable PoC devices. Here we describe a R2R-manufacturable, user-friendly (easy-to-operate and has a shelf-life of at least 6 months), self-filling and low-cost (<1 €, materials only) PDMS assay with LAMP-based and multiplexed fluorescence detection of nucleic acids from difficult sample matrixes, such as blood, for PoC diagnostics. The assay can detect up to 3 different sepsis-causing bacteria simultaneously (limit of detection, LOD, is 500 fg) from low-volume blood sample (2 μL) without pre-treatment in less than 1 h.

Original languageEnglish
Title of host publicationBioCAS 2022 - IEEE Biomedical Circuits and Systems Conference
Subtitle of host publicationIntelligent Biomedical Systems for a Better Future, Proceedings
PublisherIEEE Institute of Electrical and Electronic Engineers
ISBN (Electronic)978-1-6654-6917-3
ISBN (Print)978-1-6654-6918-0
Publication statusPublished - 2022
MoE publication typeA4 Article in a conference publication
EventIEEE Biomedical Circuits and Systems Conference, BioCAS 2022 - Taipei, Taiwan, Province of China
Duration: 13 Oct 202215 Oct 2022


ConferenceIEEE Biomedical Circuits and Systems Conference, BioCAS 2022
Country/TerritoryTaiwan, Province of China


  • diagnostics
  • lab-on-chip
  • LAMP
  • loop-mediated isothermal amplification
  • nucleic acid amplification
  • pathogen
  • PDMS
  • point-of-care
  • polydimethylsiloxane
  • R2R
  • roll-to-roll


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