Roll-to-roll fabricated self-filling polydimethylsiloxane diagnostic platforms for multiplexed pathogen nucleic acid detection

Lauri Rannaste; Olli Heikki Huttunen; Johanna Hiitola-Keinänen; Jussi Hiltunen; Christina Liedert; Leena Hakalahti

doi:10.1109/BioCAS54905.2022.9948648

Roll-to-roll fabricated self-filling polydimethylsiloxane diagnostic platforms for multiplexed pathogen nucleic acid detection

Lauri Rannaste, Olli Heikki Huttunen, Johanna Hiitola-Keinänen, Jussi Hiltunen, Christina Liedert, Leena Hakalahti

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Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific › peer-review

1 Citation (Scopus)

Abstract

Polydimethylsiloxane (PDMS) is a commonly used material for prototyping microfluidics due to its biocompatibility, transparency, elasticity, low auto-fluorescence, and low cost in comparison to similar materials. PDMS-based devices provide alternatives for conventional diagnostic materials, such as polymerase chain reaction (PCR) tubes, that are limited in supply due to high demand in pandemics or similar situations. Roll-to-roll (R2R) production enables mass production of PDMS-based devices for point-of-care (PoC) diagnostics of pathogen nucleic acids. Mass manufacturing and end user points-of-view must be considered when designing a new assay. Isothermal nucleic acid amplification techniques enable less complex heating solutions. Self-filling of the device without external equipment, such as vacuum pumps, is also necessary for user-friendly and portable PoC devices. Here we describe a R2R-manufacturable, user-friendly (easy-to-operate and has a shelf-life of at least 6 months), self-filling and low-cost (<1 €, materials only) PDMS assay with LAMP-based and multiplexed fluorescence detection of nucleic acids from difficult sample matrixes, such as blood, for PoC diagnostics. The assay can detect up to 3 different sepsis-causing bacteria simultaneously (limit of detection, LOD, is 500 fg) from low-volume blood sample (2 μL) without pre-treatment in less than 1 h.

Original language	English
Title of host publication	BioCAS 2022 - IEEE Biomedical Circuits and Systems Conference
Subtitle of host publication	Intelligent Biomedical Systems for a Better Future, Proceedings
Publisher	IEEE Institute of Electrical and Electronic Engineers
Pages	115-119
ISBN (Electronic)	978-1-6654-6917-3
ISBN (Print)	978-1-6654-6918-0
DOIs	https://doi.org/10.1109/BioCAS54905.2022.9948648
Publication status	Published - 2022
MoE publication type	A4 Article in a conference publication
Event	IEEE Biomedical Circuits and Systems Conference, BioCAS 2022 - Taipei, Taiwan, Province of China Duration: 13 Oct 2022 → 15 Oct 2022

Conference

Conference	IEEE Biomedical Circuits and Systems Conference, BioCAS 2022
Country/Territory	Taiwan, Province of China
City	Taipei
Period	13/10/22 → 15/10/22

Keywords

diagnostics
lab-on-chip
LAMP
loop-mediated isothermal amplification
nucleic acid amplification
pathogen
PDMS
point-of-care
polydimethylsiloxane
R2R
roll-to-roll

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1109/BioCAS54905.2022.9948648

Cite this

Rannaste, L., Huttunen, O. H., Hiitola-Keinänen, J., Hiltunen, J., Liedert, C., & Hakalahti, L. (2022). Roll-to-roll fabricated self-filling polydimethylsiloxane diagnostic platforms for multiplexed pathogen nucleic acid detection. In BioCAS 2022 - IEEE Biomedical Circuits and Systems Conference: Intelligent Biomedical Systems for a Better Future, Proceedings (pp. 115-119). IEEE Institute of Electrical and Electronic Engineers. https://doi.org/10.1109/BioCAS54905.2022.9948648

Rannaste, Lauri ; Huttunen, Olli Heikki ; Hiitola-Keinänen, Johanna et al. / Roll-to-roll fabricated self-filling polydimethylsiloxane diagnostic platforms for multiplexed pathogen nucleic acid detection. BioCAS 2022 - IEEE Biomedical Circuits and Systems Conference: Intelligent Biomedical Systems for a Better Future, Proceedings. IEEE Institute of Electrical and Electronic Engineers, 2022. pp. 115-119

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title = "Roll-to-roll fabricated self-filling polydimethylsiloxane diagnostic platforms for multiplexed pathogen nucleic acid detection",

abstract = "Polydimethylsiloxane (PDMS) is a commonly used material for prototyping microfluidics due to its biocompatibility, transparency, elasticity, low auto-fluorescence, and low cost in comparison to similar materials. PDMS-based devices provide alternatives for conventional diagnostic materials, such as polymerase chain reaction (PCR) tubes, that are limited in supply due to high demand in pandemics or similar situations. Roll-to-roll (R2R) production enables mass production of PDMS-based devices for point-of-care (PoC) diagnostics of pathogen nucleic acids. Mass manufacturing and end user points-of-view must be considered when designing a new assay. Isothermal nucleic acid amplification techniques enable less complex heating solutions. Self-filling of the device without external equipment, such as vacuum pumps, is also necessary for user-friendly and portable PoC devices. Here we describe a R2R-manufacturable, user-friendly (easy-to-operate and has a shelf-life of at least 6 months), self-filling and low-cost (<1 €, materials only) PDMS assay with LAMP-based and multiplexed fluorescence detection of nucleic acids from difficult sample matrixes, such as blood, for PoC diagnostics. The assay can detect up to 3 different sepsis-causing bacteria simultaneously (limit of detection, LOD, is 500 fg) from low-volume blood sample (2 μL) without pre-treatment in less than 1 h.",

keywords = "diagnostics, lab-on-chip, LAMP, loop-mediated isothermal amplification, nucleic acid amplification, pathogen, PDMS, point-of-care, polydimethylsiloxane, R2R, roll-to-roll",

author = "Lauri Rannaste and Huttunen, \{Olli Heikki\} and Johanna Hiitola-Kein{\"a}nen and Jussi Hiltunen and Christina Liedert and Leena Hakalahti",

note = "Publisher Copyright: {\textcopyright} 2022 IEEE.; IEEE Biomedical Circuits and Systems Conference, BioCAS 2022 ; Conference date: 13-10-2022 Through 15-10-2022",

year = "2022",

doi = "10.1109/BioCAS54905.2022.9948648",

language = "English",

isbn = "978-1-6654-6918-0",

pages = "115--119",

booktitle = "BioCAS 2022 - IEEE Biomedical Circuits and Systems Conference",

publisher = "IEEE Institute of Electrical and Electronic Engineers",

address = "United States",

}

Rannaste, L , Huttunen, OH , Hiitola-Keinänen, J , Hiltunen, J , Liedert, C & Hakalahti, L 2022, Roll-to-roll fabricated self-filling polydimethylsiloxane diagnostic platforms for multiplexed pathogen nucleic acid detection. in BioCAS 2022 - IEEE Biomedical Circuits and Systems Conference: Intelligent Biomedical Systems for a Better Future, Proceedings. IEEE Institute of Electrical and Electronic Engineers, pp. 115-119, IEEE Biomedical Circuits and Systems Conference, BioCAS 2022, Taipei, Taiwan, Province of China, 13/10/22. https://doi.org/10.1109/BioCAS54905.2022.9948648

Roll-to-roll fabricated self-filling polydimethylsiloxane diagnostic platforms for multiplexed pathogen nucleic acid detection. / Rannaste, Lauri ; Huttunen, Olli Heikki ; Hiitola-Keinänen, Johanna et al.
BioCAS 2022 - IEEE Biomedical Circuits and Systems Conference: Intelligent Biomedical Systems for a Better Future, Proceedings. IEEE Institute of Electrical and Electronic Engineers, 2022. p. 115-119.

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific › peer-review

TY - GEN

T1 - Roll-to-roll fabricated self-filling polydimethylsiloxane diagnostic platforms for multiplexed pathogen nucleic acid detection

AU - Rannaste, Lauri

AU - Huttunen, Olli Heikki

AU - Hiitola-Keinänen, Johanna

AU - Hiltunen, Jussi

AU - Liedert, Christina

AU - Hakalahti, Leena

PY - 2022

Y1 - 2022

N2 - Polydimethylsiloxane (PDMS) is a commonly used material for prototyping microfluidics due to its biocompatibility, transparency, elasticity, low auto-fluorescence, and low cost in comparison to similar materials. PDMS-based devices provide alternatives for conventional diagnostic materials, such as polymerase chain reaction (PCR) tubes, that are limited in supply due to high demand in pandemics or similar situations. Roll-to-roll (R2R) production enables mass production of PDMS-based devices for point-of-care (PoC) diagnostics of pathogen nucleic acids. Mass manufacturing and end user points-of-view must be considered when designing a new assay. Isothermal nucleic acid amplification techniques enable less complex heating solutions. Self-filling of the device without external equipment, such as vacuum pumps, is also necessary for user-friendly and portable PoC devices. Here we describe a R2R-manufacturable, user-friendly (easy-to-operate and has a shelf-life of at least 6 months), self-filling and low-cost (<1 €, materials only) PDMS assay with LAMP-based and multiplexed fluorescence detection of nucleic acids from difficult sample matrixes, such as blood, for PoC diagnostics. The assay can detect up to 3 different sepsis-causing bacteria simultaneously (limit of detection, LOD, is 500 fg) from low-volume blood sample (2 μL) without pre-treatment in less than 1 h.

AB - Polydimethylsiloxane (PDMS) is a commonly used material for prototyping microfluidics due to its biocompatibility, transparency, elasticity, low auto-fluorescence, and low cost in comparison to similar materials. PDMS-based devices provide alternatives for conventional diagnostic materials, such as polymerase chain reaction (PCR) tubes, that are limited in supply due to high demand in pandemics or similar situations. Roll-to-roll (R2R) production enables mass production of PDMS-based devices for point-of-care (PoC) diagnostics of pathogen nucleic acids. Mass manufacturing and end user points-of-view must be considered when designing a new assay. Isothermal nucleic acid amplification techniques enable less complex heating solutions. Self-filling of the device without external equipment, such as vacuum pumps, is also necessary for user-friendly and portable PoC devices. Here we describe a R2R-manufacturable, user-friendly (easy-to-operate and has a shelf-life of at least 6 months), self-filling and low-cost (<1 €, materials only) PDMS assay with LAMP-based and multiplexed fluorescence detection of nucleic acids from difficult sample matrixes, such as blood, for PoC diagnostics. The assay can detect up to 3 different sepsis-causing bacteria simultaneously (limit of detection, LOD, is 500 fg) from low-volume blood sample (2 μL) without pre-treatment in less than 1 h.

KW - diagnostics

KW - lab-on-chip

KW - LAMP

KW - loop-mediated isothermal amplification

KW - nucleic acid amplification

KW - pathogen

KW - PDMS

KW - point-of-care

KW - polydimethylsiloxane

KW - R2R

KW - roll-to-roll

UR - https://www.scopus.com/pages/publications/85142928939

U2 - 10.1109/BioCAS54905.2022.9948648

DO - 10.1109/BioCAS54905.2022.9948648

M3 - Conference article in proceedings

AN - SCOPUS:85142928939

SN - 978-1-6654-6918-0

SP - 115

EP - 119

BT - BioCAS 2022 - IEEE Biomedical Circuits and Systems Conference

PB - IEEE Institute of Electrical and Electronic Engineers

T2 - IEEE Biomedical Circuits and Systems Conference, BioCAS 2022

Y2 - 13 October 2022 through 15 October 2022

ER -

Rannaste L , Huttunen OH , Hiitola-Keinänen J , Hiltunen J , Liedert C, Hakalahti L. Roll-to-roll fabricated self-filling polydimethylsiloxane diagnostic platforms for multiplexed pathogen nucleic acid detection. In BioCAS 2022 - IEEE Biomedical Circuits and Systems Conference: Intelligent Biomedical Systems for a Better Future, Proceedings. IEEE Institute of Electrical and Electronic Engineers. 2022. p. 115-119 doi: 10.1109/BioCAS54905.2022.9948648

Roll-to-roll fabricated self-filling polydimethylsiloxane diagnostic platforms for multiplexed pathogen nucleic acid detection

Abstract

Conference

Keywords

UN SDGs

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