Roll-to-roll fabrication of integrated PDMS–paper microfluidics for nucleic acid amplification

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6 Citations (Scopus)

Abstract

Microfluidic-based integrated molecular diagnostic systems, which are automated, sensitive, specific, user-friendly, robust, rapid, easy-to-use, and portable, can revolutionize future medicine. Current research and development largely relies on polydimethylsiloxane (PDMS) to fabricate microfluidic devices. Since the transition from the proof-of-principle phase to clinical studies requires a vast number of integrated microfluidic devices, there is a need for a high-volume manufacturing method of silicone-based microfluidics. Here we present the first roll-to-roll (R2R) thermal imprinting method to fabricate integrated PDMS–paper microfluidics for molecular diagnostics, which allows production of tens of thousands of replicates in an hour. In order to validate the replicated molecular diagnostic platforms, on-chip amplification of viral ribonucleic acid (RNA) with loop-mediated isothermal amplification (LAMP) was demonstrated. These low-cost, rapid and accurate molecular diagnostic platforms will generate a wide range of applications in preventive personalized medicine, global healthcare, agriculture, food, environment, water monitoring, and global biosecurity.
Original languageEnglish
Pages (from-to)1552-1559
Number of pages8
JournalLab on a Chip
Volume18
Issue number11
Early online date30 Apr 2018
DOIs
Publication statusPublished - 2018
MoE publication typeA1 Journal article-refereed

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Microfluidics
Molecular Pathology
Nucleic acids
Lab-On-A-Chip Devices
Nucleic Acids
Amplification
Fabrication
Medicine
Precision Medicine
Preventive Medicine
Silicones
Agriculture
Polydimethylsiloxane
RNA
Hot Temperature
Delivery of Health Care
Costs and Cost Analysis
Food
Water
Research

Cite this

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title = "Roll-to-roll fabrication of integrated PDMS–paper microfluidics for nucleic acid amplification",
abstract = "Microfluidic-based integrated molecular diagnostic systems, which are automated, sensitive, specific, user-friendly, robust, rapid, easy-to-use, and portable, can revolutionize future medicine. Current research and development largely relies on polydimethylsiloxane (PDMS) to fabricate microfluidic devices. Since the transition from the proof-of-principle phase to clinical studies requires a vast number of integrated microfluidic devices, there is a need for a high-volume manufacturing method of silicone-based microfluidics. Here we present the first roll-to-roll (R2R) thermal imprinting method to fabricate integrated PDMS–paper microfluidics for molecular diagnostics, which allows production of tens of thousands of replicates in an hour. In order to validate the replicated molecular diagnostic platforms, on-chip amplification of viral ribonucleic acid (RNA) with loop-mediated isothermal amplification (LAMP) was demonstrated. These low-cost, rapid and accurate molecular diagnostic platforms will generate a wide range of applications in preventive personalized medicine, global healthcare, agriculture, food, environment, water monitoring, and global biosecurity.",
author = "Jussi Hiltunen and Christina Liedert and Marianne Hiltunen and Olli-Heikki Huttunen and Johanna Hiitola-Kein{\"a}nen and Sanna Aikio and Mikko Harjanne and Marika Kurkinen and Leena Hakalahti and Lee, {Luke P.}",
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T1 - Roll-to-roll fabrication of integrated PDMS–paper microfluidics for nucleic acid amplification

AU - Hiltunen, Jussi

AU - Liedert, Christina

AU - Hiltunen, Marianne

AU - Huttunen, Olli-Heikki

AU - Hiitola-Keinänen, Johanna

AU - Aikio, Sanna

AU - Harjanne, Mikko

AU - Kurkinen, Marika

AU - Hakalahti, Leena

AU - Lee, Luke P.

PY - 2018

Y1 - 2018

N2 - Microfluidic-based integrated molecular diagnostic systems, which are automated, sensitive, specific, user-friendly, robust, rapid, easy-to-use, and portable, can revolutionize future medicine. Current research and development largely relies on polydimethylsiloxane (PDMS) to fabricate microfluidic devices. Since the transition from the proof-of-principle phase to clinical studies requires a vast number of integrated microfluidic devices, there is a need for a high-volume manufacturing method of silicone-based microfluidics. Here we present the first roll-to-roll (R2R) thermal imprinting method to fabricate integrated PDMS–paper microfluidics for molecular diagnostics, which allows production of tens of thousands of replicates in an hour. In order to validate the replicated molecular diagnostic platforms, on-chip amplification of viral ribonucleic acid (RNA) with loop-mediated isothermal amplification (LAMP) was demonstrated. These low-cost, rapid and accurate molecular diagnostic platforms will generate a wide range of applications in preventive personalized medicine, global healthcare, agriculture, food, environment, water monitoring, and global biosecurity.

AB - Microfluidic-based integrated molecular diagnostic systems, which are automated, sensitive, specific, user-friendly, robust, rapid, easy-to-use, and portable, can revolutionize future medicine. Current research and development largely relies on polydimethylsiloxane (PDMS) to fabricate microfluidic devices. Since the transition from the proof-of-principle phase to clinical studies requires a vast number of integrated microfluidic devices, there is a need for a high-volume manufacturing method of silicone-based microfluidics. Here we present the first roll-to-roll (R2R) thermal imprinting method to fabricate integrated PDMS–paper microfluidics for molecular diagnostics, which allows production of tens of thousands of replicates in an hour. In order to validate the replicated molecular diagnostic platforms, on-chip amplification of viral ribonucleic acid (RNA) with loop-mediated isothermal amplification (LAMP) was demonstrated. These low-cost, rapid and accurate molecular diagnostic platforms will generate a wide range of applications in preventive personalized medicine, global healthcare, agriculture, food, environment, water monitoring, and global biosecurity.

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JO - Lab on a Chip

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