Roll-to-Roll Manufacturing of Integrated Immunodetection Sensors

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Lack of functional integration and high manufacturing costs have been identified as major challenges in commercialization of point-of-care devices. In this study, roll-to-roll (R2R) fabrication process was developed for large-scale manufacturing of disposable microfluidic devices. The integrated, user-friendly device included a plasma separation membrane for simple blood filtration, immobilized antibodies for specific immunodetection, microfluidics for plasma transport and reagent mixing, and a blister for actuation and waste storage. These functionalities were designed to be compatible with R2R processing, which was demonstrated using pilot-scale printing lines producing 60 devices in an hour. The produced sensors enabled rapid (10 min) and sensitive (2 μg/mL) fluorescence-based immunodetection of C-reactive protein from 20 μL of whole blood.

Original languageEnglish
Pages (from-to)2010-2017
Number of pages8
JournalACS Sensors
Volume5
Issue number7
DOIs
Publication statusPublished - 24 Jul 2020
MoE publication typeA1 Journal article-refereed

Keywords

  • blood filtration
  • cyclic olefin copolymer
  • immunoassay
  • microfluidics
  • point-of-care
  • roll-to-roll manufacturing

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