@article{c721114a2397460d9130d2e4388abb5c,
title = "Roll-to-Roll Manufacturing of Integrated Immunodetection Sensors",
abstract = "Lack of functional integration and high manufacturing costs have been identified as major challenges in commercialization of point-of-care devices. In this study, roll-to-roll (R2R) fabrication process was developed for large-scale manufacturing of disposable microfluidic devices. The integrated, user-friendly device included a plasma separation membrane for simple blood filtration, immobilized antibodies for specific immunodetection, microfluidics for plasma transport and reagent mixing, and a blister for actuation and waste storage. These functionalities were designed to be compatible with R2R processing, which was demonstrated using pilot-scale printing lines producing 60 devices in an hour. The produced sensors enabled rapid (10 min) and sensitive (2 μg/mL) fluorescence-based immunodetection of C-reactive protein from 20 μL of whole blood.",
keywords = "blood filtration, cyclic olefin copolymer, immunoassay, microfluidics, point-of-care, roll-to-roll manufacturing",
author = "Christina Liedert and Lauri Rannaste and Annukka Kokkonen and Olli-Heikki Huttunen and Ralph Liedert and Jussi Hiltunen and Leena Hakalahti",
note = "Funding Information: The authors are grateful for the financial support from the European Regional Development Fund for the project “Developing production methods for rapid diagnostics and sample handling”. Some of the facilities used were provided by the Academy of Finland Research Infrastructure Printed Intelligence Infrastructure (PII-FIRI, grant no. 320020). This work is a part of the Academy of Finland Flagship Program, Photonics Research and Innovation (PREIN), decision 320168. We also wish to acknowledge VTT{\textquoteright}s pilot line operators and laboratory technicians and engineers, Jaakko Pennanen, Pentti Korhonen, Mikko Hietala, Pekka Ontero, Jenni Tomperi, and Sari Pohjonen, for their valuable assistance. Publisher Copyright: Copyright {\textcopyright} 2020 American Chemical Society. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",
year = "2020",
month = jul,
day = "24",
doi = "10.1021/acssensors.0c00404",
language = "English",
volume = "5",
pages = "2010--2017",
journal = "ACS Sensors",
issn = "2379-3694",
publisher = "American Chemical Society ACS",
number = "7",
}