Roll-to-Roll Manufacturing of Integrated Immunodetection Sensors

Christina Liedert; Lauri Rannaste; Annukka Kokkonen; Olli-Heikki Huttunen; Ralph Liedert; Jussi Hiltunen; Leena Hakalahti

doi:10.1021/acssensors.0c00404

Roll-to-Roll Manufacturing of Integrated Immunodetection Sensors

Christina Liedert^*
, Lauri Rannaste
, Annukka Kokkonen
, Olli-Heikki Huttunen
, Ralph Liedert
, Jussi Hiltunen
, Leena Hakalahti

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

59 Citations (Scopus)

Abstract

Lack of functional integration and high manufacturing costs have been identified as major challenges in commercialization of point-of-care devices. In this study, roll-to-roll (R2R) fabrication process was developed for large-scale manufacturing of disposable microfluidic devices. The integrated, user-friendly device included a plasma separation membrane for simple blood filtration, immobilized antibodies for specific immunodetection, microfluidics for plasma transport and reagent mixing, and a blister for actuation and waste storage. These functionalities were designed to be compatible with R2R processing, which was demonstrated using pilot-scale printing lines producing 60 devices in an hour. The produced sensors enabled rapid (10 min) and sensitive (2 μg/mL) fluorescence-based immunodetection of C-reactive protein from 20 μL of whole blood.

Original language	English
Pages (from-to)	2010-2017
Number of pages	8
Journal	ACS Sensors
Volume	5
Issue number	7
DOIs	https://doi.org/10.1021/acssensors.0c00404
Publication status	Published - 24 Jul 2020
MoE publication type	A1 Journal article-refereed

Funding

The authors are grateful for the financial support from the European Regional Development Fund for the project “Developing production methods for rapid diagnostics and sample handling”. Some of the facilities used were provided by the Academy of Finland Research Infrastructure Printed Intelligence Infrastructure (PII-FIRI, grant no. 320020). This work is a part of the Academy of Finland Flagship Program, Photonics Research and Innovation (PREIN), decision 320168.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 9 Industry, Innovation, and Infrastructure

Keywords

blood filtration
cyclic olefin copolymer
immunoassay
microfluidics
point-of-care
roll-to-roll manufacturing

Access to Document

10.1021/acssensors.0c00404

Cite this

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abstract = "Lack of functional integration and high manufacturing costs have been identified as major challenges in commercialization of point-of-care devices. In this study, roll-to-roll (R2R) fabrication process was developed for large-scale manufacturing of disposable microfluidic devices. The integrated, user-friendly device included a plasma separation membrane for simple blood filtration, immobilized antibodies for specific immunodetection, microfluidics for plasma transport and reagent mixing, and a blister for actuation and waste storage. These functionalities were designed to be compatible with R2R processing, which was demonstrated using pilot-scale printing lines producing 60 devices in an hour. The produced sensors enabled rapid (10 min) and sensitive (2 μg/mL) fluorescence-based immunodetection of C-reactive protein from 20 μL of whole blood.",

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AU - Liedert, Christina

AU - Rannaste, Lauri

AU - Kokkonen, Annukka

AU - Huttunen, Olli-Heikki

AU - Liedert, Ralph

AU - Hiltunen, Jussi

AU - Hakalahti, Leena

PY - 2020/7/24

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AB - Lack of functional integration and high manufacturing costs have been identified as major challenges in commercialization of point-of-care devices. In this study, roll-to-roll (R2R) fabrication process was developed for large-scale manufacturing of disposable microfluidic devices. The integrated, user-friendly device included a plasma separation membrane for simple blood filtration, immobilized antibodies for specific immunodetection, microfluidics for plasma transport and reagent mixing, and a blister for actuation and waste storage. These functionalities were designed to be compatible with R2R processing, which was demonstrated using pilot-scale printing lines producing 60 devices in an hour. The produced sensors enabled rapid (10 min) and sensitive (2 μg/mL) fluorescence-based immunodetection of C-reactive protein from 20 μL of whole blood.

KW - blood filtration

KW - cyclic olefin copolymer

KW - immunoassay

KW - microfluidics

KW - point-of-care

KW - roll-to-roll manufacturing

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Roll-to-Roll Manufacturing of Integrated Immunodetection Sensors

Abstract

Funding

UN SDGs

Keywords

Access to Document

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