Abstract
Lack of functional integration and high manufacturing costs have been identified as major challenges in commercialization of point-of-care devices. In this study, roll-to-roll (R2R) fabrication process was developed for large-scale manufacturing of disposable microfluidic devices. The integrated, user-friendly device included a plasma separation membrane for simple blood filtration, immobilized antibodies for specific immunodetection, microfluidics for plasma transport and reagent mixing, and a blister for actuation and waste storage. These functionalities were designed to be compatible with R2R processing, which was demonstrated using pilot-scale printing lines producing 60 devices in an hour. The produced sensors enabled rapid (10 min) and sensitive (2 μg/mL) fluorescence-based immunodetection of C-reactive protein from 20 μL of whole blood.
| Original language | English |
|---|---|
| Pages (from-to) | 2010-2017 |
| Number of pages | 8 |
| Journal | ACS Sensors |
| Volume | 5 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 24 Jul 2020 |
| MoE publication type | A1 Journal article-refereed |
Funding
The authors are grateful for the financial support from the European Regional Development Fund for the project “Developing production methods for rapid diagnostics and sample handling”. Some of the facilities used were provided by the Academy of Finland Research Infrastructure Printed Intelligence Infrastructure (PII-FIRI, grant no. 320020). This work is a part of the Academy of Finland Flagship Program, Photonics Research and Innovation (PREIN), decision 320168.
Keywords
- blood filtration
- cyclic olefin copolymer
- immunoassay
- microfluidics
- point-of-care
- roll-to-roll manufacturing
