SAFTIR - a novel point-of-need diagnostic platform for animal health and food safety

Mikko Utriainen (Corresponding author), Petri Saviranta, Sanna Aikio, Marianne Hiltunen, Asko Kokkonen, Aki Mäyrä, Markku Alamäki, Hannu Vasama, Kirsi Tappura

    Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientificpeer-review

    Abstract

    This paper introduces a portable rapid molecular diagnostics device concept based on unique and novel SAFTIR (Supercritical Angle Fluorescence and Total Internal Reflection) optical transducer technology. The device platform is compatible with numerous fluorescence assay chemistries for performing rapid tests on-site. The SAFTIR technology has been developed at VTT Technical Research Centre of Finland utilising our know-how in biosensors, integrated optics, electronics and biosensor prototyping. The uniqueness of the SAFTIR prototype device is based on the novel measurement principle involving integrated optical elements and waveguides on the disposable test strip and the specific reader device with proper interface optics. At concept level, SAFTIR has a lot of similarities with the Lateral Flow Assay (LFA) based rapid tests by consisting of an analyte specific disposable test strip and a special reader device. However, in technical details, there are significant differences and benefits, namely SAFTIR utilises surface-bound fluorescence detection principle ensuring high sensitivity with an excellent signal-to-noise ratio, fast response time and minimal sample matrix effects. In this paper, we present for the first time the integrated SAFTIR device concept, portable prototype and development aiming at smart farming applications. The initial application focus is on-site detection and quantitation of mycotoxins for both arable and livestock farming. The reference and starting point is a previously developed assay based on a recombinant anti-immunocomplex antibody for HT-2 mycotoxin detection.

    Original languageEnglish
    Title of host publicationPrecision Livestock Farming 2019
    Subtitle of host publicationPapers Presented at the 9th European Conference on Precision Livestock Farming, ECPLF 2019
    EditorsBernadette O'Brien, Deirdre Hennessy, Laurence Shalloo
    Pages721-726
    Number of pages6
    Publication statusPublished - 1 Jan 2019
    MoE publication typeA4 Article in a conference publication
    Event9th European Conference on Precision Livestock Farming, ECPLF 2019 - Cork, Ireland
    Duration: 26 Aug 201929 Aug 2019

    Conference

    Conference9th European Conference on Precision Livestock Farming, ECPLF 2019
    CountryIreland
    CityCork
    Period26/08/1929/08/19

    Keywords

    • Fluorescence assay
    • HT-2
    • Molecular diagnostic
    • Mycotoxin
    • Rapid test
    • OtaNano

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