TY - JOUR
T1 - Sambucus nigra L. cell cultures produce main species-specific phytochemicals with anti-inflammatory properties and in vitro ACE2 binding inhibition to SARS-CoV2
AU - Borodušķe, Anete
AU - Balode, Madara
AU - Nakurte, Ilva
AU - Berga, Marta
AU - Jēkabsons, Kaspars
AU - Muceniece, Ruta
AU - Rischer, Heiko
N1 - Funding Information:
This work was supported by ERDF , University of Latvia, and Republic of Latvia post-doctoral grant number 1.1.1.2./ VIAA/2/18/256 “Biotechnological solutions for control of target compounds in European elder Sambucus nigra in vitro cell cultures and in planta using endophytic microorganisms”.
Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/10/15
Y1 - 2022/10/15
N2 - Sambucus nigra fruit and flower extracts exhibit anti-viral and anti-inflammatory properties however, the potential of S. nigra in vitro cell suspension cultures to produce biologically active compounds remains unexplored. This study describes the establishment of S. nigra cell suspension cultures and their phytochemical content and biological activity. S. nigra cell cultures contain major compounds characteristic for whole-plant S. nigra tissues albeit at uncharacteristic ratios. Elicitation with methyl jasmonate, visual selection of pigmented cells, and optimization of culture parameters resulted in cell suspensions with high contents of anthocyanins, organic acids, and phenolic compounds. Enzyme-linked immunosorbent assay (ELISA) and fluorometric assay-based assessment of biological activity of developed cultures demonstrated inhibitory capacity against cyclooxygenase enzyme COX2 activity and against the binding of SARS-CoV2 receptor-binding domain (RBD) to angiotensin-converting enzyme 2 (ACE2) in vitro suggesting that S. nigra cell suspensions exhibit anti-inflammatory and viral entry blocking potential. Differences in phytochemical content and biological activity of S. nigra cell suspensions subjected to different optimization approaches indicate potential for optimization towards desired biotechnological products with anti-inflammatory properties and inhibitory capacity against viral binding.
AB - Sambucus nigra fruit and flower extracts exhibit anti-viral and anti-inflammatory properties however, the potential of S. nigra in vitro cell suspension cultures to produce biologically active compounds remains unexplored. This study describes the establishment of S. nigra cell suspension cultures and their phytochemical content and biological activity. S. nigra cell cultures contain major compounds characteristic for whole-plant S. nigra tissues albeit at uncharacteristic ratios. Elicitation with methyl jasmonate, visual selection of pigmented cells, and optimization of culture parameters resulted in cell suspensions with high contents of anthocyanins, organic acids, and phenolic compounds. Enzyme-linked immunosorbent assay (ELISA) and fluorometric assay-based assessment of biological activity of developed cultures demonstrated inhibitory capacity against cyclooxygenase enzyme COX2 activity and against the binding of SARS-CoV2 receptor-binding domain (RBD) to angiotensin-converting enzyme 2 (ACE2) in vitro suggesting that S. nigra cell suspensions exhibit anti-inflammatory and viral entry blocking potential. Differences in phytochemical content and biological activity of S. nigra cell suspensions subjected to different optimization approaches indicate potential for optimization towards desired biotechnological products with anti-inflammatory properties and inhibitory capacity against viral binding.
KW - COX2 inhibition
KW - Elderberry
KW - MeJA elicitation
KW - Plant cell suspensions culture
KW - SARS CoV2 binding to ACE2
UR - http://www.scopus.com/inward/record.url?scp=85133270811&partnerID=8YFLogxK
U2 - 10.1016/j.indcrop.2022.115236
DO - 10.1016/j.indcrop.2022.115236
M3 - Article
AN - SCOPUS:85133270811
SN - 0926-6690
VL - 186
JO - Industrial Crops and Products
JF - Industrial Crops and Products
M1 - 115236
ER -