Sambucus nigra L. cell cultures produce main species-specific phytochemicals with anti-inflammatory properties and in vitro ACE2 binding inhibition to SARS-CoV2

Anete Borodušķe (Corresponding Author), Madara Balode, Ilva Nakurte, Marta Berga, Kaspars Jēkabsons, Ruta Muceniece, Heiko Rischer

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Sambucus nigra fruit and flower extracts exhibit anti-viral and anti-inflammatory properties however, the potential of S. nigra in vitro cell suspension cultures to produce biologically active compounds remains unexplored. This study describes the establishment of S. nigra cell suspension cultures and their phytochemical content and biological activity. S. nigra cell cultures contain major compounds characteristic for whole-plant S. nigra tissues albeit at uncharacteristic ratios. Elicitation with methyl jasmonate, visual selection of pigmented cells, and optimization of culture parameters resulted in cell suspensions with high contents of anthocyanins, organic acids, and phenolic compounds. Enzyme-linked immunosorbent assay (ELISA) and fluorometric assay-based assessment of biological activity of developed cultures demonstrated inhibitory capacity against cyclooxygenase enzyme COX2 activity and against the binding of SARS-CoV2 receptor-binding domain (RBD) to angiotensin-converting enzyme 2 (ACE2) in vitro suggesting that S. nigra cell suspensions exhibit anti-inflammatory and viral entry blocking potential. Differences in phytochemical content and biological activity of S. nigra cell suspensions subjected to different optimization approaches indicate potential for optimization towards desired biotechnological products with anti-inflammatory properties and inhibitory capacity against viral binding.

Original languageEnglish
Article number115236
Number of pages11
JournalIndustrial Crops and Products
Volume186
Early online date17 Jul 2022
DOIs
Publication statusE-pub ahead of print - 17 Jul 2022
MoE publication typeA1 Journal article-refereed

Keywords

  • COX2 inhibition
  • Elderberry
  • MeJA elicitation
  • Plant cell suspensions culture
  • SARS CoV2 binding to ACE2

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