TY - JOUR
T1 - Sanguiin H-6 fractionated from cloudberry (Rubus chamaemorus) seeds can prevent the methicillin-resistant staphylococcus aureus biofilm development during wound infection
AU - Aguilera-Correa, John Jairo
AU - Fernández-López, Sara
AU - Cuñas-Figueroa, Iskra Dennisse
AU - Pérez-Rial, Sandra
AU - Alakomi, Hanna Leena
AU - Nohynek, Liisa
AU - Oksman-Caldentey, Kirsi Marja
AU - Salminen, Juha Pekka
AU - Esteban, Jaime
AU - Cuadros, Juan
AU - Puupponen-Pimiä, Riitta
AU - Perez-Tanoira, Ramon
AU - Kinnari, Teemu J.
N1 - Funding Information:
Conflicts of Interest: J.E. received travel grants from Pfizer and conference fees from Biomérieux and Heraeus. The remaining authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.
Funding Information:
Funding: This research was funded by VTT Innovation program iBEX, Innovative Business to solve Exponential problems, during the years 2018–2019. Open access funding provided by University of Helsinki.
Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/12/3
Y1 - 2021/12/3
N2 - Staphylococcus aureus is the most common cause of surgical site infections and its treatment is challenging due to the emergence of multi-drug resistant strains such as methicillin-resistant S. aureus (MRSA). Natural berry-derived compounds have shown antimicrobial potential, e.g., ellagitannins such as sanguiin H-6 and lambertianin C, the main phenolic compounds in Rubus seeds, have shown antimicrobial activity. The aim of this study was to evaluate the effect of sanguiin H-6 and lambertianin C fractionated from cloudberry seeds, on the MRSA growth, and as treatment of a MRSA biofilm development in different growth media in vitro and in vivo by using a murine wound infection model where sanguiin H-6 and lambertianin C were used to prevent the MRSA infection. Sanguiin H-6 and lambertianin C inhibited the in vitro biofilm development and growth of MRSA. Furthermore, sanguiin H-6 showed significant anti-MRSA effect in the in vivo wound model. Our study shows the possible use of sanguiin H-6 as a preventive measure in surgical sites to avoid postoperative infections, whilst lambertianin C showed no anti-MRSA activity.
AB - Staphylococcus aureus is the most common cause of surgical site infections and its treatment is challenging due to the emergence of multi-drug resistant strains such as methicillin-resistant S. aureus (MRSA). Natural berry-derived compounds have shown antimicrobial potential, e.g., ellagitannins such as sanguiin H-6 and lambertianin C, the main phenolic compounds in Rubus seeds, have shown antimicrobial activity. The aim of this study was to evaluate the effect of sanguiin H-6 and lambertianin C fractionated from cloudberry seeds, on the MRSA growth, and as treatment of a MRSA biofilm development in different growth media in vitro and in vivo by using a murine wound infection model where sanguiin H-6 and lambertianin C were used to prevent the MRSA infection. Sanguiin H-6 and lambertianin C inhibited the in vitro biofilm development and growth of MRSA. Furthermore, sanguiin H-6 showed significant anti-MRSA effect in the in vivo wound model. Our study shows the possible use of sanguiin H-6 as a preventive measure in surgical sites to avoid postoperative infections, whilst lambertianin C showed no anti-MRSA activity.
KW - Biofilm
KW - Cloudberry
KW - Ellagitannin
KW - Methicillin-resistant S. aureus
KW - Rubus
UR - http://www.scopus.com/inward/record.url?scp=85121577670&partnerID=8YFLogxK
U2 - 10.3390/antibiotics10121481
DO - 10.3390/antibiotics10121481
M3 - Article
AN - SCOPUS:85121577670
SN - 2079-6382
VL - 10
JO - Antibiotics
JF - Antibiotics
IS - 12
M1 - 1481
ER -
