Screening for novel laccase-producing microbes

Laura-Leena Kiiskinen, Marjaana Rättö, Kristiina Kruus

    Research output: Contribution to journalArticleScientificpeer-review

    160 Citations (Scopus)

    Abstract

    Aims: To discover novel laccases potential for industrial applications.
    Methods and Results: Fungi were cultivated on solid media containing indicator compounds that enabled the detection of laccases as specific colour reactions. The indicators used were Remazol Brilliant Blue R (RBBR), Poly R-478, guaiacol and tannic acid. The screening work resulted in isolation of 26 positive fungal strains. Liquid cultivations of positive strains confirmed that four efficient laccase producers were found in the screening. Biochemical characteristics of the four novel laccases were typical for fungal laccases in terms of molecular weight, pH optima and pI. The laccases showed good thermal stability at 60°C.
    Conclusions: Plate-test screening based on polymeric dye compounds, guaiacol and tannic acid is an efficient way to discover novel laccase producers. The results indicated that screening for laccase activity can be performed with guaiacol and RBBR or Poly R-478.
    Significance and Impact of the Study: Laccases have many potential industrial applications including textile dye decolourization, delignification of pulp and effluent detoxification. It is essential to find novel, efficient enzymes to further develop these applications. This study showed that relatively simple plate test screening method can be used for discovery of novel laccases.
    Original languageEnglish
    Pages (from-to)640-646
    JournalJournal of Applied Microbiology
    Volume97
    Issue number3
    DOIs
    Publication statusPublished - 2004
    MoE publication typeA1 Journal article-refereed

    Keywords

    • guaiacol
    • laccase, Poly R-478
    • RBBR
    • screening
    • tannic acid

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