Screening of glycoside hydrolases and ionic liquids for fibre modification

Jenni Rahikainen, Sasikala Anbarasan, Ronny Wahlström, Arno Parviainen, Alistair W.T. King, Terhi Puranen, Kristiina Kruus, Ilkka Kilpeläinen, Ossi Turunen, Anna Suurnäkki

    Research output: Contribution to journalArticleScientificpeer-review

    3 Citations (Scopus)


    BACKGROUND: This study elaborates the possibility to apply combined ionic liquid (IL) and enzyme treatments for pulp fibre modification. The approach involves swelling of fibre surfaces with IL followed by enzymatic modification of the disrupted fibre surface using carbohydrate active enzymes. RESULTS: The capacity of seven cellulose-dissolving or cellulose-swelling ionic liquids to swell pulp fibres was compared. In addition, thirteen cellulases and five xylanases were screened for their IL tolerance, which determines their applicability in combined or sequential IL–enzyme treatments of fibres. Among the studied ionic liquids, 1-ethyl-3-methylimidazolium dimethylphosphate ([EMIM]DMP) and 1,3-dimethylimidazolium dimethylphosphate ([DMIM]DMP) had the strongest effect on fibre swelling. These solvents were also found to be the least inactivating for the studied enzymes. CONCLUSION: Enzyme compatibility and cellulose-dissolving capability are not two conflicting properties of an ionic liquid.

    Original languageEnglish
    Pages (from-to)818-826
    Number of pages9
    JournalJournal of Chemical Technology and Biotechnology
    Issue number3
    Early online date2017
    Publication statusPublished - 2018
    MoE publication typeA1 Journal article-refereed


    • ionic liquid
    • cellulase
    • endoglucanase
    • xylanase
    • fibre modification
    • CBM


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