Screening of glycoside hydrolases and ionic liquids for fibre modification

Jenni Rahikainen, Sasikala Anbarasan, Ronny Wahlström, Arno Parviainen, Alistair W.T. King, Terhi Puranen, Kristiina Kruus, Ilkka Kilpeläinen, Ossi Turunen, Anna Suurnäkki

Research output: Contribution to journalArticleScientificpeer-review

Abstract

BACKGROUND: This study elaborates the possibility to apply combined ionic liquid (IL) and enzyme treatments for pulp fibre modification. The approach involves swelling of fibre surfaces with IL followed by enzymatic modification of the disrupted fibre surface using carbohydrate active enzymes. RESULTS: The capacity of seven cellulose-dissolving or cellulose-swelling ionic liquids to swell pulp fibres was compared. In addition, thirteen cellulases and five xylanases were screened for their IL tolerance, which determines their applicability in combined or sequential IL–enzyme treatments of fibres. Among the studied ionic liquids, 1-ethyl-3-methylimidazolium dimethylphosphate ([EMIM]DMP) and 1,3-dimethylimidazolium dimethylphosphate ([DMIM]DMP) had the strongest effect on fibre swelling. These solvents were also found to be the least inactivating for the studied enzymes. CONCLUSION: Enzyme compatibility and cellulose-dissolving capability are not two conflicting properties of an ionic liquid.

Original languageEnglish
Pages (from-to)818-826
Number of pages9
JournalJournal of Chemical Technology and Biotechnology
Volume93
Issue number3
Early online date2017
DOIs
Publication statusPublished - 2018
MoE publication typeA1 Journal article-refereed

Fingerprint

Ionic Liquids
Glycoside Hydrolases
Ionic liquids
Screening
Fibers
Enzymes
Cellulose
swelling
enzyme
Swelling
cellulose
Pulp
Cellulases
Carbohydrates
swell
ionic liquid
fibre
screening
carbohydrate
tolerance

Keywords

  • ionic liquid
  • cellulase
  • endoglucanase
  • xylanase
  • fibre modification
  • CBM

Cite this

Rahikainen, J., Anbarasan, S., Wahlström, R., Parviainen, A., King, A. W. T., Puranen, T., ... Suurnäkki, A. (2018). Screening of glycoside hydrolases and ionic liquids for fibre modification. Journal of Chemical Technology and Biotechnology, 93(3), 818-826. https://doi.org/10.1002/jctb.5435
Rahikainen, Jenni ; Anbarasan, Sasikala ; Wahlström, Ronny ; Parviainen, Arno ; King, Alistair W.T. ; Puranen, Terhi ; Kruus, Kristiina ; Kilpeläinen, Ilkka ; Turunen, Ossi ; Suurnäkki, Anna. / Screening of glycoside hydrolases and ionic liquids for fibre modification. In: Journal of Chemical Technology and Biotechnology. 2018 ; Vol. 93, No. 3. pp. 818-826.
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abstract = "BACKGROUND: This study elaborates the possibility to apply combined ionic liquid (IL) and enzyme treatments for pulp fibre modification. The approach involves swelling of fibre surfaces with IL followed by enzymatic modification of the disrupted fibre surface using carbohydrate active enzymes. RESULTS: The capacity of seven cellulose-dissolving or cellulose-swelling ionic liquids to swell pulp fibres was compared. In addition, thirteen cellulases and five xylanases were screened for their IL tolerance, which determines their applicability in combined or sequential IL–enzyme treatments of fibres. Among the studied ionic liquids, 1-ethyl-3-methylimidazolium dimethylphosphate ([EMIM]DMP) and 1,3-dimethylimidazolium dimethylphosphate ([DMIM]DMP) had the strongest effect on fibre swelling. These solvents were also found to be the least inactivating for the studied enzymes. CONCLUSION: Enzyme compatibility and cellulose-dissolving capability are not two conflicting properties of an ionic liquid.",
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Rahikainen, J, Anbarasan, S, Wahlström, R, Parviainen, A, King, AWT, Puranen, T, Kruus, K, Kilpeläinen, I, Turunen, O & Suurnäkki, A 2018, 'Screening of glycoside hydrolases and ionic liquids for fibre modification', Journal of Chemical Technology and Biotechnology, vol. 93, no. 3, pp. 818-826. https://doi.org/10.1002/jctb.5435

Screening of glycoside hydrolases and ionic liquids for fibre modification. / Rahikainen, Jenni; Anbarasan, Sasikala; Wahlström, Ronny; Parviainen, Arno; King, Alistair W.T.; Puranen, Terhi; Kruus, Kristiina; Kilpeläinen, Ilkka; Turunen, Ossi; Suurnäkki, Anna.

In: Journal of Chemical Technology and Biotechnology, Vol. 93, No. 3, 2018, p. 818-826.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Screening of glycoside hydrolases and ionic liquids for fibre modification

AU - Rahikainen, Jenni

AU - Anbarasan, Sasikala

AU - Wahlström, Ronny

AU - Parviainen, Arno

AU - King, Alistair W.T.

AU - Puranen, Terhi

AU - Kruus, Kristiina

AU - Kilpeläinen, Ilkka

AU - Turunen, Ossi

AU - Suurnäkki, Anna

PY - 2018

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AB - BACKGROUND: This study elaborates the possibility to apply combined ionic liquid (IL) and enzyme treatments for pulp fibre modification. The approach involves swelling of fibre surfaces with IL followed by enzymatic modification of the disrupted fibre surface using carbohydrate active enzymes. RESULTS: The capacity of seven cellulose-dissolving or cellulose-swelling ionic liquids to swell pulp fibres was compared. In addition, thirteen cellulases and five xylanases were screened for their IL tolerance, which determines their applicability in combined or sequential IL–enzyme treatments of fibres. Among the studied ionic liquids, 1-ethyl-3-methylimidazolium dimethylphosphate ([EMIM]DMP) and 1,3-dimethylimidazolium dimethylphosphate ([DMIM]DMP) had the strongest effect on fibre swelling. These solvents were also found to be the least inactivating for the studied enzymes. CONCLUSION: Enzyme compatibility and cellulose-dissolving capability are not two conflicting properties of an ionic liquid.

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