Screening of microbes for lipases specific for saturated medium and long-chain fatty acids of milk fat

Hanna Miettinen, Antti Nyyssölä, s. Rokka, Hanna Kontkanen, Kristiina Kruus (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

5 Citations (Scopus)

Abstract

Over 250 microbial strains were screened for lipase activity specific towards saturated medium and long-chain fatty acids. Strains showing trimyristin (C14:0) hydrolysis on agar medium were further streaked on tricaprylin (C8:0) and on olive oil (mostly C18:1) agar to identify strains hydrolysing C12–C15 fatty acids with modest or no activity towards short-chain and unsaturated fatty acids. The twenty strains showing the desired specificity were grown as liquid cultures and analysed for p-nitrophenyl (p-NP) myristate hydrolysis in the presence of Triton X-100. Cells from the cultures showing the highest activity towards p-NP myristate were analysed for specificity towards p-NP esters of different fatty acid chain lengths. On the basis of the p-NP ester profiling, four lipase samples were selected for hydrolysis experiments on butter oil. Of these, the sample obtained from the cultivation of Didpodascus capitatus met the screening criteria: C12:0–C16:0 specificity and no preference towards C18:0 and C18:1 fatty acids.
Original languageEnglish
Pages (from-to)61-67
Number of pages6
JournalInternational Dairy Journal
Volume32
Issue number2
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

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medium chain fatty acids
long chain fatty acids
Lipase
milk fat
saturated fatty acids
Milk
Fatty Acids
Fats
screening
microorganisms
Hydrolysis
hydrolysis
Myristic Acid
Agar
Esters
agar
butter oil
fatty acids
fatty acid esters
Volatile Fatty Acids

Cite this

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title = "Screening of microbes for lipases specific for saturated medium and long-chain fatty acids of milk fat",
abstract = "Over 250 microbial strains were screened for lipase activity specific towards saturated medium and long-chain fatty acids. Strains showing trimyristin (C14:0) hydrolysis on agar medium were further streaked on tricaprylin (C8:0) and on olive oil (mostly C18:1) agar to identify strains hydrolysing C12–C15 fatty acids with modest or no activity towards short-chain and unsaturated fatty acids. The twenty strains showing the desired specificity were grown as liquid cultures and analysed for p-nitrophenyl (p-NP) myristate hydrolysis in the presence of Triton X-100. Cells from the cultures showing the highest activity towards p-NP myristate were analysed for specificity towards p-NP esters of different fatty acid chain lengths. On the basis of the p-NP ester profiling, four lipase samples were selected for hydrolysis experiments on butter oil. Of these, the sample obtained from the cultivation of Didpodascus capitatus met the screening criteria: C12:0–C16:0 specificity and no preference towards C18:0 and C18:1 fatty acids.",
author = "Hanna Miettinen and Antti Nyyss{\"o}l{\"a} and s. Rokka and Hanna Kontkanen and Kristiina Kruus",
year = "2013",
doi = "10.1016/j.idairyj.2013.05.007",
language = "English",
volume = "32",
pages = "61--67",
journal = "International Dairy Journal",
issn = "0958-6946",
publisher = "Elsevier",
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Screening of microbes for lipases specific for saturated medium and long-chain fatty acids of milk fat. / Miettinen, Hanna; Nyyssölä, Antti; Rokka, s.; Kontkanen, Hanna; Kruus, Kristiina (Corresponding Author).

In: International Dairy Journal, Vol. 32, No. 2, 2013, p. 61-67.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

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AU - Miettinen, Hanna

AU - Nyyssölä, Antti

AU - Rokka, s.

AU - Kontkanen, Hanna

AU - Kruus, Kristiina

PY - 2013

Y1 - 2013

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AB - Over 250 microbial strains were screened for lipase activity specific towards saturated medium and long-chain fatty acids. Strains showing trimyristin (C14:0) hydrolysis on agar medium were further streaked on tricaprylin (C8:0) and on olive oil (mostly C18:1) agar to identify strains hydrolysing C12–C15 fatty acids with modest or no activity towards short-chain and unsaturated fatty acids. The twenty strains showing the desired specificity were grown as liquid cultures and analysed for p-nitrophenyl (p-NP) myristate hydrolysis in the presence of Triton X-100. Cells from the cultures showing the highest activity towards p-NP myristate were analysed for specificity towards p-NP esters of different fatty acid chain lengths. On the basis of the p-NP ester profiling, four lipase samples were selected for hydrolysis experiments on butter oil. Of these, the sample obtained from the cultivation of Didpodascus capitatus met the screening criteria: C12:0–C16:0 specificity and no preference towards C18:0 and C18:1 fatty acids.

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