Screening of novel fungal Carbohydrate Esterase family 1 enzymes identifies three novel dual feruloyl/acetyl xylan esterases

Adiphol Dilokpimol, Bart Verkerk, Xinxin Li, Annie Bellemare, Mathieu Lavallee, Matthias Frommhagen, Emilie Nørmølle Underlin, Mirjam A. Kabel, Justin Powlowski, Adrian Tsang, Ronald P. de Vries (Corresponding Author)

    Research output: Contribution to journalArticleScientificpeer-review

    Abstract

    Feruloyl esterases (FAEs) and acetyl xylan esterases (AXEs) are important enzymes for plant biomass degradation and are both present in Carbohydrate Esterase family 1 (CE1) of the Carbohydrate-Active enZymes database. In this study, ten novel fungal CE1 enzymes from different subfamilies were heterologously produced and screened for their activity towards model and complex plant biomass substrates. CE1_1 enzymes possess AXE activity, while CE1_5 enzymes showed FAE activity. Two enzymes from CE1_2 and one from CE1_5 possess dual feruloyl/acetyl xylan esterase (FXE) activity, showing expansion of substrate specificity. The new FXEs from CE1 can efficiently release both feruloyl and acetyl residues from feruloylated xylan, making them particularly interesting novel components of industrial enzyme cocktails for plant biomass degradation.

    Original languageEnglish
    Number of pages12
    JournalFEBS Letters
    DOIs
    Publication statusE-pub ahead of print - 21 Feb 2022
    MoE publication typeA1 Journal article-refereed

    Keywords

    • acetyl xylan esterase
    • carbohydrate esterase family 1
    • feruloyl esterase
    • fungi
    • hydroxycinnamic acid
    • plant biomass

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