Sec9 is a SNAP-25-like component of a yeast SNARE complex that may be the effector of Sec4 function in exocytosis

Patrick Brennwald, Brian Kearns, Kathy Champion, Sirkka Keränen, Vytas Bankaitis, Peter Novick

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305 Citations (Scopus)

Abstract

To identify potential Sec4 effectors, we isolated high copy suppressors of a Sec4 effector domain mutant. The most potent of these was found to be SEC9, a gene required for post-Golgi transport. The sole essential domain of Sec9 has significant sequence similarity to the neuronal protein SNAP-25, a component of the SNARE complex, that is implicated in vesicle targeting and fusion. Analogous to SNAP-25, Sec9 is bound to the yeast plasma membrane and is absent from post-Golgi vesicles. Furthermore, Sec9 is physically associated with two proteins that are homologous to components of the neuronal SNARE complex. Our results identify Sec9 as the yeast cognate of SNAP-25 and suggest that SNARE complexes acting at specific stages of vesicular transport serve as the ultimate targets of regulation by members of the Sec4/Ypt1/Rab family of GTPases.
Original languageEnglish
Pages (from-to)245-258
JournalCell
Volume79
Issue number2
DOIs
Publication statusPublished - 21 Oct 1994
MoE publication typeA1 Journal article-refereed

Keywords

  • Sec9
  • SNAP-25
  • Sec-4

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    Brennwald, P., Kearns, B., Champion, K., Keränen, S., Bankaitis, V., & Novick, P. (1994). Sec9 is a SNAP-25-like component of a yeast SNARE complex that may be the effector of Sec4 function in exocytosis. Cell, 79(2), 245-258. https://doi.org/10.1016/0092-8674(94)90194-5