Secondary metabolism in root and callus cultures of Hyoscyamus muticus L.

The relationship between morphological organisation and response to methyl jasmonate

Stefania Biondi (Corresponding Author), Sonia Scaramagli, Kirsi-Marja Oksman-Caldentey, Ferruccio Poli

Research output: Contribution to journalArticleScientificpeer-review

29 Citations (Scopus)

Abstract

Production of acid-soluble conjugated di- and polyamines, like that of other secondary metabolites, is enhanced by exposure to methyl jasmonate (MJ). We investigated this metabolic response, and activities of enzymes involved in putrescine (Put) and tropane alkaloid biosynthesis, in root cultures of Hyoscyamus muticus and compared it with that of callus cultures.
In root cultures, free Put and N-methylputrescine (mPut) increased upon treatment with MJ, whereas in callus cultures mPut levels were not affected. Differently from roots, conjugated amines were scarce or absent in callus cultures, and accumulated only transiently upon treatment with MJ.
Arginine decarboxylase, ornithine decarboxylase and diamine oxidase activities in root cultures were strongly stimulated by treatment with MJ, but were inhibited in callus cultures. Exposure to MJ also enhanced putrescine N-methyltransferase activity in root cultures more than in callus cultures.
These results are discussed in relation to the different capacity for tropane alkaloid production in the two culture systems.
Original languageEnglish
Pages (from-to)563-569
JournalPlant Science
Volume163
Issue number3
DOIs
Publication statusPublished - 2002
MoE publication typeA1 Journal article-refereed

Fingerprint

Hyoscyamus
Hyoscyamus muticus
Secondary Metabolism
Bony Callus
methyl jasmonate
callus culture
metabolism
Tropanes
tropane alkaloids
Putrescine
putrescine N-methyltransferase
putrescine
Alkaloids
Amine Oxidase (Copper-Containing)
arginine decarboxylase
ornithine decarboxylase
diamines
Ornithine Decarboxylase
Diamines
Polyamines

Keywords

  • Hyoscyamus muticus
  • Methyl jasmonate
  • Polyamine metabolism
  • Tissue culture
  • Tropane alkaloids

Cite this

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title = "Secondary metabolism in root and callus cultures of Hyoscyamus muticus L.: The relationship between morphological organisation and response to methyl jasmonate",
abstract = "Production of acid-soluble conjugated di- and polyamines, like that of other secondary metabolites, is enhanced by exposure to methyl jasmonate (MJ). We investigated this metabolic response, and activities of enzymes involved in putrescine (Put) and tropane alkaloid biosynthesis, in root cultures of Hyoscyamus muticus and compared it with that of callus cultures. In root cultures, free Put and N-methylputrescine (mPut) increased upon treatment with MJ, whereas in callus cultures mPut levels were not affected. Differently from roots, conjugated amines were scarce or absent in callus cultures, and accumulated only transiently upon treatment with MJ. Arginine decarboxylase, ornithine decarboxylase and diamine oxidase activities in root cultures were strongly stimulated by treatment with MJ, but were inhibited in callus cultures. Exposure to MJ also enhanced putrescine N-methyltransferase activity in root cultures more than in callus cultures. These results are discussed in relation to the different capacity for tropane alkaloid production in the two culture systems.",
keywords = "Hyoscyamus muticus, Methyl jasmonate, Polyamine metabolism, Tissue culture, Tropane alkaloids",
author = "Stefania Biondi and Sonia Scaramagli and Kirsi-Marja Oksman-Caldentey and Ferruccio Poli",
year = "2002",
doi = "10.1016/S0168-9452(02)00161-9",
language = "English",
volume = "163",
pages = "563--569",
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Secondary metabolism in root and callus cultures of Hyoscyamus muticus L. The relationship between morphological organisation and response to methyl jasmonate. / Biondi, Stefania (Corresponding Author); Scaramagli, Sonia; Oksman-Caldentey, Kirsi-Marja; Poli, Ferruccio.

In: Plant Science, Vol. 163, No. 3, 2002, p. 563-569.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Secondary metabolism in root and callus cultures of Hyoscyamus muticus L.

T2 - The relationship between morphological organisation and response to methyl jasmonate

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AU - Oksman-Caldentey, Kirsi-Marja

AU - Poli, Ferruccio

PY - 2002

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AB - Production of acid-soluble conjugated di- and polyamines, like that of other secondary metabolites, is enhanced by exposure to methyl jasmonate (MJ). We investigated this metabolic response, and activities of enzymes involved in putrescine (Put) and tropane alkaloid biosynthesis, in root cultures of Hyoscyamus muticus and compared it with that of callus cultures. In root cultures, free Put and N-methylputrescine (mPut) increased upon treatment with MJ, whereas in callus cultures mPut levels were not affected. Differently from roots, conjugated amines were scarce or absent in callus cultures, and accumulated only transiently upon treatment with MJ. Arginine decarboxylase, ornithine decarboxylase and diamine oxidase activities in root cultures were strongly stimulated by treatment with MJ, but were inhibited in callus cultures. Exposure to MJ also enhanced putrescine N-methyltransferase activity in root cultures more than in callus cultures. These results are discussed in relation to the different capacity for tropane alkaloid production in the two culture systems.

KW - Hyoscyamus muticus

KW - Methyl jasmonate

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KW - Tissue culture

KW - Tropane alkaloids

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DO - 10.1016/S0168-9452(02)00161-9

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