Secondary metabolism in root and callus cultures of Hyoscyamus muticus L. The relationship between morphological organisation and response to methyl jasmonate

Stefania Biondi (Corresponding Author), Sonia Scaramagli, Kirsi-Marja Oksman-Caldentey, Ferruccio Poli

    Research output: Contribution to journalArticleScientificpeer-review

    32 Citations (Scopus)

    Abstract

    Production of acid-soluble conjugated di- and polyamines, like that of other secondary metabolites, is enhanced by exposure to methyl jasmonate (MJ). We investigated this metabolic response, and activities of enzymes involved in putrescine (Put) and tropane alkaloid biosynthesis, in root cultures of Hyoscyamus muticus and compared it with that of callus cultures.
    In root cultures, free Put and N-methylputrescine (mPut) increased upon treatment with MJ, whereas in callus cultures mPut levels were not affected. Differently from roots, conjugated amines were scarce or absent in callus cultures, and accumulated only transiently upon treatment with MJ.
    Arginine decarboxylase, ornithine decarboxylase and diamine oxidase activities in root cultures were strongly stimulated by treatment with MJ, but were inhibited in callus cultures. Exposure to MJ also enhanced putrescine N-methyltransferase activity in root cultures more than in callus cultures.
    These results are discussed in relation to the different capacity for tropane alkaloid production in the two culture systems.
    Original languageEnglish
    Pages (from-to)563-569
    JournalPlant Science
    Volume163
    Issue number3
    DOIs
    Publication statusPublished - 2002
    MoE publication typeA1 Journal article-refereed

    Keywords

    • Hyoscyamus muticus
    • Methyl jasmonate
    • Polyamine metabolism
    • Tissue culture
    • Tropane alkaloids

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