Secretion of green fluorescent protein from recombinant baculovirus-infected insect cells

Marja-Leena Laukkanen, Christian Oker-Blom, Kari Keinänen

Research output: Contribution to journalArticleScientificpeer-review

40 Citations (Scopus)

Abstract

Trichoplusia ni (High Five) and Spodoptera frugiperda (Sf21) cells were engineered for expression of epitope (Flag)-tagged signal peptide-green fluorescent protein (GFP) fusions to examine the suitability of GFP as a secretory marker. The recombinant baculovirus-infected cells became fluorescent, and the High Five cells but not Sf21 cells secreted GFP in the culture medium as detected by the presence in the culture supernatant of a Flag-immunoreactive 30-kDa species and the characteristic 510-nm GFP fluorescence peak. Signal peptides derived from ecdysteroid UDP-glucosyltransferase of Autographa californica nuclear polyhedrosis virus and from rat brain glutamate receptor were both able to promote secretion of GFP. GFP may thus be used as a research tool in the study of the secretory process in insect cells both in cell biology and in biotechnological applications.
Original languageEnglish
Pages (from-to)755-761
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume226
Issue number3
DOIs
Publication statusPublished - 1996
MoE publication typeA1 Journal article-refereed

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Baculoviridae
Green Fluorescent Proteins
Insects
Sf9 Cells
Protein Sorting Signals
Cytology
Nucleopolyhedrovirus
Spodoptera
Secretory Pathway
Glutamate Receptors
Viruses
Cell Biology
Culture Media
Rats
Epitopes
Brain
Fusion reactions
Fluorescence
Research

Cite this

Laukkanen, Marja-Leena ; Oker-Blom, Christian ; Keinänen, Kari. / Secretion of green fluorescent protein from recombinant baculovirus-infected insect cells. In: Biochemical and Biophysical Research Communications. 1996 ; Vol. 226, No. 3. pp. 755-761.
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abstract = "Trichoplusia ni (High Five) and Spodoptera frugiperda (Sf21) cells were engineered for expression of epitope (Flag)-tagged signal peptide-green fluorescent protein (GFP) fusions to examine the suitability of GFP as a secretory marker. The recombinant baculovirus-infected cells became fluorescent, and the High Five cells but not Sf21 cells secreted GFP in the culture medium as detected by the presence in the culture supernatant of a Flag-immunoreactive 30-kDa species and the characteristic 510-nm GFP fluorescence peak. Signal peptides derived from ecdysteroid UDP-glucosyltransferase of Autographa californica nuclear polyhedrosis virus and from rat brain glutamate receptor were both able to promote secretion of GFP. GFP may thus be used as a research tool in the study of the secretory process in insect cells both in cell biology and in biotechnological applications.",
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Secretion of green fluorescent protein from recombinant baculovirus-infected insect cells. / Laukkanen, Marja-Leena; Oker-Blom, Christian; Keinänen, Kari.

In: Biochemical and Biophysical Research Communications, Vol. 226, No. 3, 1996, p. 755-761.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Secretion of green fluorescent protein from recombinant baculovirus-infected insect cells

AU - Laukkanen, Marja-Leena

AU - Oker-Blom, Christian

AU - Keinänen, Kari

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