Secretion of green fluorescent protein from recombinant baculovirus-infected insect cells

Marja-Leena Laukkanen, Christian Oker-Blom, Kari Keinänen

    Research output: Contribution to journalArticleScientificpeer-review

    41 Citations (Scopus)


    Trichoplusia ni (High Five) and Spodoptera frugiperda (Sf21) cells were engineered for expression of epitope (Flag)-tagged signal peptide-green fluorescent protein (GFP) fusions to examine the suitability of GFP as a secretory marker. The recombinant baculovirus-infected cells became fluorescent, and the High Five cells but not Sf21 cells secreted GFP in the culture medium as detected by the presence in the culture supernatant of a Flag-immunoreactive 30-kDa species and the characteristic 510-nm GFP fluorescence peak. Signal peptides derived from ecdysteroid UDP-glucosyltransferase of Autographa californica nuclear polyhedrosis virus and from rat brain glutamate receptor were both able to promote secretion of GFP. GFP may thus be used as a research tool in the study of the secretory process in insect cells both in cell biology and in biotechnological applications.
    Original languageEnglish
    Pages (from-to)755-761
    Number of pages7
    JournalBiochemical and Biophysical Research Communications
    Issue number3
    Publication statusPublished - 1996
    MoE publication typeA1 Journal article-refereed


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