The substrate specificity of the yeast (Saccharomyces cerevisiae) pleiotropic drug resistance (PDR)-type ATP-binding cassette (ABC) transporters is extended to plant secondary metabolites of the tropane alkaloid family. Functional analysis of yeast PDR5 genes in transgenic tobacco (Nicotiana tabacum L. cv Bright-Yellow 2 [BY-2]) cell lines suggest that PDR genes can be used to stimulate the secretion of secondary metabolites in plant cell suspension cultures.
- secondary metabolites
- plant cells
- gene expression
- ABC transporters
Goossens, A., Häkkinen, S. T., Laakso, I., Oksman-Caldentey, K-M., & Inzé, D. (2003). Secretion of secondary metabolites by ATP-binding cassette transporters in plant cell suspension cultures. Plant Physiology, 131(3), 1161-1164. https://doi.org/10.1104/pp.102.016329