Secretion of secondary metabolites by ATP-binding cassette transporters in plant cell suspension cultures

Alain Goossens, Suvi T. Häkkinen, Into Laakso, Kirsi-Marja Oksman-Caldentey, Dirk Inzé

Research output: Contribution to journalArticleScientificpeer-review

48 Citations (Scopus)

Abstract

The substrate specificity of the yeast (Saccharomyces cerevisiae) pleiotropic drug resistance (PDR)-type ATP-binding cassette (ABC) transporters is extended to plant secondary metabolites of the tropane alkaloid family. Functional analysis of yeast PDR5 genes in transgenic tobacco (Nicotiana tabacum L. cv Bright-Yellow 2 [BY-2]) cell lines suggest that PDR genes can be used to stimulate the secretion of secondary metabolites in plant cell suspension cultures.
Original languageEnglish
Pages (from-to)1161-1164
JournalPlant Physiology
Volume131
Issue number3
DOIs
Publication statusPublished - 2003
MoE publication typeA1 Journal article-refereed

Fingerprint

ABC transporters
ATP-Binding Cassette Transporters
Plant Cells
drug resistance
Drug Resistance
cell suspension culture
secondary metabolites
Tobacco
Suspensions
Cell Culture Techniques
Yeasts
secretion
Tropanes
yeasts
tropane alkaloids
substrate specificity
Substrate Specificity
Alkaloids
Nicotiana tabacum
Genes

Keywords

  • metabolites
  • secondary metabolites
  • metabolomics
  • plant cells
  • ATP
  • gene expression
  • ABC transporters

Cite this

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title = "Secretion of secondary metabolites by ATP-binding cassette transporters in plant cell suspension cultures",
abstract = "The substrate specificity of the yeast (Saccharomyces cerevisiae) pleiotropic drug resistance (PDR)-type ATP-binding cassette (ABC) transporters is extended to plant secondary metabolites of the tropane alkaloid family. Functional analysis of yeast PDR5 genes in transgenic tobacco (Nicotiana tabacum L. cv Bright-Yellow 2 [BY-2]) cell lines suggest that PDR genes can be used to stimulate the secretion of secondary metabolites in plant cell suspension cultures.",
keywords = "metabolites, secondary metabolites, metabolomics, plant cells, ATP, gene expression, ABC transporters",
author = "Alain Goossens and H{\"a}kkinen, {Suvi T.} and Into Laakso and Kirsi-Marja Oksman-Caldentey and Dirk Inz{\'e}",
year = "2003",
doi = "10.1104/pp.102.016329",
language = "English",
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pages = "1161--1164",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
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}

Secretion of secondary metabolites by ATP-binding cassette transporters in plant cell suspension cultures. / Goossens, Alain; Häkkinen, Suvi T.; Laakso, Into; Oksman-Caldentey, Kirsi-Marja; Inzé, Dirk.

In: Plant Physiology, Vol. 131, No. 3, 2003, p. 1161-1164.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Secretion of secondary metabolites by ATP-binding cassette transporters in plant cell suspension cultures

AU - Goossens, Alain

AU - Häkkinen, Suvi T.

AU - Laakso, Into

AU - Oksman-Caldentey, Kirsi-Marja

AU - Inzé, Dirk

PY - 2003

Y1 - 2003

N2 - The substrate specificity of the yeast (Saccharomyces cerevisiae) pleiotropic drug resistance (PDR)-type ATP-binding cassette (ABC) transporters is extended to plant secondary metabolites of the tropane alkaloid family. Functional analysis of yeast PDR5 genes in transgenic tobacco (Nicotiana tabacum L. cv Bright-Yellow 2 [BY-2]) cell lines suggest that PDR genes can be used to stimulate the secretion of secondary metabolites in plant cell suspension cultures.

AB - The substrate specificity of the yeast (Saccharomyces cerevisiae) pleiotropic drug resistance (PDR)-type ATP-binding cassette (ABC) transporters is extended to plant secondary metabolites of the tropane alkaloid family. Functional analysis of yeast PDR5 genes in transgenic tobacco (Nicotiana tabacum L. cv Bright-Yellow 2 [BY-2]) cell lines suggest that PDR genes can be used to stimulate the secretion of secondary metabolites in plant cell suspension cultures.

KW - metabolites

KW - secondary metabolites

KW - metabolomics

KW - plant cells

KW - ATP

KW - gene expression

KW - ABC transporters

U2 - 10.1104/pp.102.016329

DO - 10.1104/pp.102.016329

M3 - Article

VL - 131

SP - 1161

EP - 1164

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 3

ER -