Segregation of AFLP markers in Betula pendula (roth)

Satu Åkerman, Jussi Tammisola, Mikko Regina, Veli Kauppinen, Seppo Lapinjoki (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

Abstract

The generation and segregation of AFLP (amplified fragment length polymorphism) markers were studied in European while birch. The recently published technique is based on PCR amplification of restriction fragments from digested total DNA by utilizing known oligomers (adapters) linked to the ends of the fragments. PCR primers partially complementary to the adapters and the restriction site are used to create the marker bands. In the present work EcoRI or TaqI were used for digestion.The primers had three arbitrary bases at the 3' ends for selective amplification of only a subset of the initial fragments. Each primer produced about 40 marker bands, of which about 10 in each case were found polymorphic between the parents of the full-sib progeny trees studied. When the segregation of alleles in the progeny was studied at 31 polymorphic loci, 29 of these were confirmed to express Mendelian ratios. The results indicate that the AFLP markers are very suitable for fingerprinting and genetic marker mapping in the birch. We found them superior to RAPD markers particularly in repeatability. The AFLP technique also produced substantially more polymorphic loci per PCR reaction. It was possible to use an automated DNA sequencer for marker band scanning after initial evaluation of the marker profiles on agarose gel.
Original languageEnglish
Pages (from-to)117-124
Number of pages8
JournalForest Genetics
Volume3
Issue number2
Publication statusPublished - 1996
MoE publication typeA1 Journal article-refereed

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Betula pendula
amplified fragment length polymorphism
Betula
gene segregation
loci
genetic markers
repeatability
agarose
digestion
gels
DNA
methodology

Cite this

Åkerman, S., Tammisola, J., Regina, M., Kauppinen, V., & Lapinjoki, S. (1996). Segregation of AFLP markers in Betula pendula (roth). Forest Genetics, 3(2), 117-124.
Åkerman, Satu ; Tammisola, Jussi ; Regina, Mikko ; Kauppinen, Veli ; Lapinjoki, Seppo. / Segregation of AFLP markers in Betula pendula (roth). In: Forest Genetics. 1996 ; Vol. 3, No. 2. pp. 117-124.
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Åkerman, S, Tammisola, J, Regina, M, Kauppinen, V & Lapinjoki, S 1996, 'Segregation of AFLP markers in Betula pendula (roth)', Forest Genetics, vol. 3, no. 2, pp. 117-124.

Segregation of AFLP markers in Betula pendula (roth). / Åkerman, Satu; Tammisola, Jussi; Regina, Mikko; Kauppinen, Veli; Lapinjoki, Seppo (Corresponding Author).

In: Forest Genetics, Vol. 3, No. 2, 1996, p. 117-124.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Lapinjoki, Seppo

N1 - Project code: BIO2035

PY - 1996

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N2 - The generation and segregation of AFLP (amplified fragment length polymorphism) markers were studied in European while birch. The recently published technique is based on PCR amplification of restriction fragments from digested total DNA by utilizing known oligomers (adapters) linked to the ends of the fragments. PCR primers partially complementary to the adapters and the restriction site are used to create the marker bands. In the present work EcoRI or TaqI were used for digestion.The primers had three arbitrary bases at the 3' ends for selective amplification of only a subset of the initial fragments. Each primer produced about 40 marker bands, of which about 10 in each case were found polymorphic between the parents of the full-sib progeny trees studied. When the segregation of alleles in the progeny was studied at 31 polymorphic loci, 29 of these were confirmed to express Mendelian ratios. The results indicate that the AFLP markers are very suitable for fingerprinting and genetic marker mapping in the birch. We found them superior to RAPD markers particularly in repeatability. The AFLP technique also produced substantially more polymorphic loci per PCR reaction. It was possible to use an automated DNA sequencer for marker band scanning after initial evaluation of the marker profiles on agarose gel.

AB - The generation and segregation of AFLP (amplified fragment length polymorphism) markers were studied in European while birch. The recently published technique is based on PCR amplification of restriction fragments from digested total DNA by utilizing known oligomers (adapters) linked to the ends of the fragments. PCR primers partially complementary to the adapters and the restriction site are used to create the marker bands. In the present work EcoRI or TaqI were used for digestion.The primers had three arbitrary bases at the 3' ends for selective amplification of only a subset of the initial fragments. Each primer produced about 40 marker bands, of which about 10 in each case were found polymorphic between the parents of the full-sib progeny trees studied. When the segregation of alleles in the progeny was studied at 31 polymorphic loci, 29 of these were confirmed to express Mendelian ratios. The results indicate that the AFLP markers are very suitable for fingerprinting and genetic marker mapping in the birch. We found them superior to RAPD markers particularly in repeatability. The AFLP technique also produced substantially more polymorphic loci per PCR reaction. It was possible to use an automated DNA sequencer for marker band scanning after initial evaluation of the marker profiles on agarose gel.

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Åkerman S, Tammisola J, Regina M, Kauppinen V, Lapinjoki S. Segregation of AFLP markers in Betula pendula (roth). Forest Genetics. 1996;3(2):117-124.