Selective nanopatterning using citrate-stabilized Au nanoparticles and cystein-modified amphiphilic protein

Päivi Laaksonen (Corresponding Author), Jani Kivioja, Arja Paananen, Markku Kainlauri, Kyösti Kontturi, Jouni Ahopelto, Markus Linder

Research output: Contribution to journalArticleScientificpeer-review

25 Citations (Scopus)

Abstract

We present an approach where biomolecular self-assembly is used in combination with lithography to produce patterns of metallic nanoparticles on a silicon substrate. This is achieved through a two-step method, resulting in attachment of nanoparticles on desired sites on the sample surfaces, which allowed a detailed characterization. First, a genetically modified hydrophobin protein, NCysHFBI, was attached by self-assembly on a hydrophobic surface or a surface patterned with hydrophobic and hydrophilic domains. The next step was to label the protein layers with 17.8 nm gold nanoparticles, to allow microscopic characterization of the films. Kinetics and extent of attachment of nanoparticles were characterized by UV−vis spectroscopy and transmission electron microscopy. It was shown that the attachment of citrate-stabilized gold nanoparticles was strongly dependent on the electrostatic properties of the capping ligand layer and the density of nanoparticles in the monolayer could be controlled via pH. The resulting nanoparticle assemblies followed the original pattern created by optical lithography in high accuracy. We demonstrate that combining bottom-up and top-down nanotechnological approaches in a good balance can provide very effective ways to produce nanoscale components providing a functional interface between electronics and the biological world.
Original languageEnglish
Pages (from-to)5185-5192
Number of pages8
JournalLangmuir
Volume25
Issue number9
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

Fingerprint

citrates
Citric Acid
Nanoparticles
proteins
Proteins
nanoparticles
attachment
Gold
Self assembly
self assembly
lithography
gold
Photolithography
Silicon
Lithography
assemblies
Labels
Electrostatics
Monolayers
Electronic equipment

Cite this

Laaksonen, Päivi ; Kivioja, Jani ; Paananen, Arja ; Kainlauri, Markku ; Kontturi, Kyösti ; Ahopelto, Jouni ; Linder, Markus. / Selective nanopatterning using citrate-stabilized Au nanoparticles and cystein-modified amphiphilic protein. In: Langmuir. 2009 ; Vol. 25, No. 9. pp. 5185-5192.
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abstract = "We present an approach where biomolecular self-assembly is used in combination with lithography to produce patterns of metallic nanoparticles on a silicon substrate. This is achieved through a two-step method, resulting in attachment of nanoparticles on desired sites on the sample surfaces, which allowed a detailed characterization. First, a genetically modified hydrophobin protein, NCysHFBI, was attached by self-assembly on a hydrophobic surface or a surface patterned with hydrophobic and hydrophilic domains. The next step was to label the protein layers with 17.8 nm gold nanoparticles, to allow microscopic characterization of the films. Kinetics and extent of attachment of nanoparticles were characterized by UV−vis spectroscopy and transmission electron microscopy. It was shown that the attachment of citrate-stabilized gold nanoparticles was strongly dependent on the electrostatic properties of the capping ligand layer and the density of nanoparticles in the monolayer could be controlled via pH. The resulting nanoparticle assemblies followed the original pattern created by optical lithography in high accuracy. We demonstrate that combining bottom-up and top-down nanotechnological approaches in a good balance can provide very effective ways to produce nanoscale components providing a functional interface between electronics and the biological world.",
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Selective nanopatterning using citrate-stabilized Au nanoparticles and cystein-modified amphiphilic protein. / Laaksonen, Päivi (Corresponding Author); Kivioja, Jani; Paananen, Arja; Kainlauri, Markku; Kontturi, Kyösti; Ahopelto, Jouni; Linder, Markus.

In: Langmuir, Vol. 25, No. 9, 2009, p. 5185-5192.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Selective nanopatterning using citrate-stabilized Au nanoparticles and cystein-modified amphiphilic protein

AU - Laaksonen, Päivi

AU - Kivioja, Jani

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AU - Kontturi, Kyösti

AU - Ahopelto, Jouni

AU - Linder, Markus

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N2 - We present an approach where biomolecular self-assembly is used in combination with lithography to produce patterns of metallic nanoparticles on a silicon substrate. This is achieved through a two-step method, resulting in attachment of nanoparticles on desired sites on the sample surfaces, which allowed a detailed characterization. First, a genetically modified hydrophobin protein, NCysHFBI, was attached by self-assembly on a hydrophobic surface or a surface patterned with hydrophobic and hydrophilic domains. The next step was to label the protein layers with 17.8 nm gold nanoparticles, to allow microscopic characterization of the films. Kinetics and extent of attachment of nanoparticles were characterized by UV−vis spectroscopy and transmission electron microscopy. It was shown that the attachment of citrate-stabilized gold nanoparticles was strongly dependent on the electrostatic properties of the capping ligand layer and the density of nanoparticles in the monolayer could be controlled via pH. The resulting nanoparticle assemblies followed the original pattern created by optical lithography in high accuracy. We demonstrate that combining bottom-up and top-down nanotechnological approaches in a good balance can provide very effective ways to produce nanoscale components providing a functional interface between electronics and the biological world.

AB - We present an approach where biomolecular self-assembly is used in combination with lithography to produce patterns of metallic nanoparticles on a silicon substrate. This is achieved through a two-step method, resulting in attachment of nanoparticles on desired sites on the sample surfaces, which allowed a detailed characterization. First, a genetically modified hydrophobin protein, NCysHFBI, was attached by self-assembly on a hydrophobic surface or a surface patterned with hydrophobic and hydrophilic domains. The next step was to label the protein layers with 17.8 nm gold nanoparticles, to allow microscopic characterization of the films. Kinetics and extent of attachment of nanoparticles were characterized by UV−vis spectroscopy and transmission electron microscopy. It was shown that the attachment of citrate-stabilized gold nanoparticles was strongly dependent on the electrostatic properties of the capping ligand layer and the density of nanoparticles in the monolayer could be controlled via pH. The resulting nanoparticle assemblies followed the original pattern created by optical lithography in high accuracy. We demonstrate that combining bottom-up and top-down nanotechnological approaches in a good balance can provide very effective ways to produce nanoscale components providing a functional interface between electronics and the biological world.

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