Hydrophobins are small proteins that function in the growth and development of fungi. The structures of class II hydrophobins HFBI and HFBII from Trichoderma reesei were studied using grazing incidence X-ray diffraction. HFBI was weakly ordered but HFBII formed a highly crystalline coating on water surface. Change from monoclinic to hexagonal structure was observed as the sample dried. The three-dimensional structures differed from the oblique two-dimensional structures observed in Langmuir-Blodgett monolayers of both HFBI and HFBII by atomic force microscopy.
- grazing-incidence X-ray diffraction
Serimaa, R., Torkkeli, M., Paananen, A., Linder, M., Kisko, K., Knaapila, M., Ikkala, O., Vuorimaa, E., Lemmetyinen, H., & Seeck, O. (2003). Self-assembled structures of hydrophobins HFBI and HFBII. Journal of Applied Crystallography, 36, 499-502. https://doi.org/10.1107/S0021889803000578