Self-Assembling Protein-Polymer Bioconjugates for Surfaces with Antifouling Features and Low Nonspecific Binding

Yingying Liu; Tarja K. Nevanen; Arja Paananen; Kristian Kempe; Paul Wilson; Leena Sisko Johansson; Jussi J. Joensuu; Markus B. Linder; David M. Haddleton; Roberto Milani

doi:10.1021/acsami.8b19968

Self-Assembling Protein-Polymer Bioconjugates for Surfaces with Antifouling Features and Low Nonspecific Binding

Yingying Liu, Tarja K. Nevanen, Arja Paananen, Kristian Kempe, Paul Wilson, Leena Sisko Johansson, Jussi J. Joensuu, Markus B. Linder, David M. Haddleton, Roberto Milani^* (Corresponding Author)

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

21 Citations (Scopus)

Abstract

A new method is demonstrated for preparing antifouling and low nonspecific adsorption surfaces on poorly reactive hydrophobic substrates, without the need for energy-intensive or environmentally aggressive pretreatments. The surface-active protein hydrophobin was covalently modified with a controlled radical polymerization initiator and allowed to self-assemble as a monolayer on hydrophobic surfaces, followed by the preparation of antifouling surfaces by Cu(0)-mediated living radical polymerization of poly(ethylene glycol) methyl ether acrylate (PEGA) performed in situ. By taking advantage of hydrophobins to achieve at the same time the immobilization of protein A, this approach allowed to prepare surfaces for IgG1 binding featuring greatly reduced nonspecific adsorption. The success of the surface modification strategy was investigated by contact angle, XPS, and AFM characterization, while the antifouling performance and the reduction of nonspecific binding were confirmed by QCM-D measurements.

Original language	English
Pages (from-to)	3599-3608
Journal	ACS Applied Materials & Interfaces
Volume	11
Issue number	3
DOIs	https://doi.org/10.1021/acsami.8b19968
Publication status	Published - 23 Jan 2019
MoE publication type	Not Eligible

Funding

This work was performed in collaboration with the Academy of Finland’s Centres of Excellence Programme (2014−2019) and is supported by the Academy of Finland (project SelfSmart, decision no. 276537 and 284508). K.K. gratefully acknowledges the award of a NHMRC-ARC Dementia Research Development Fellowship (APP1109945).

Keywords

antifouling surfaces
biosensors
hydrophobin
nonspecific binding
surface-initiated living radical polymerization

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10.1021/acsami.8b19968

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abstract = "A new method is demonstrated for preparing antifouling and low nonspecific adsorption surfaces on poorly reactive hydrophobic substrates, without the need for energy-intensive or environmentally aggressive pretreatments. The surface-active protein hydrophobin was covalently modified with a controlled radical polymerization initiator and allowed to self-assemble as a monolayer on hydrophobic surfaces, followed by the preparation of antifouling surfaces by Cu(0)-mediated living radical polymerization of poly(ethylene glycol) methyl ether acrylate (PEGA) performed in situ. By taking advantage of hydrophobins to achieve at the same time the immobilization of protein A, this approach allowed to prepare surfaces for IgG1 binding featuring greatly reduced nonspecific adsorption. The success of the surface modification strategy was investigated by contact angle, XPS, and AFM characterization, while the antifouling performance and the reduction of nonspecific binding were confirmed by QCM-D measurements.",

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AU - Nevanen, Tarja K.

AU - Paananen, Arja

AU - Kempe, Kristian

AU - Wilson, Paul

AU - Johansson, Leena Sisko

AU - Joensuu, Jussi J.

AU - Linder, Markus B.

AU - Haddleton, David M.

AU - Milani, Roberto

PY - 2019/1/23

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AB - A new method is demonstrated for preparing antifouling and low nonspecific adsorption surfaces on poorly reactive hydrophobic substrates, without the need for energy-intensive or environmentally aggressive pretreatments. The surface-active protein hydrophobin was covalently modified with a controlled radical polymerization initiator and allowed to self-assemble as a monolayer on hydrophobic surfaces, followed by the preparation of antifouling surfaces by Cu(0)-mediated living radical polymerization of poly(ethylene glycol) methyl ether acrylate (PEGA) performed in situ. By taking advantage of hydrophobins to achieve at the same time the immobilization of protein A, this approach allowed to prepare surfaces for IgG1 binding featuring greatly reduced nonspecific adsorption. The success of the surface modification strategy was investigated by contact angle, XPS, and AFM characterization, while the antifouling performance and the reduction of nonspecific binding were confirmed by QCM-D measurements.

KW - antifouling surfaces

KW - biosensors

KW - hydrophobin

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Self-Assembling Protein-Polymer Bioconjugates for Surfaces with Antifouling Features and Low Nonspecific Binding

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