Self-Assembling Protein-Polymer Bioconjugates for Surfaces with Antifouling Features and Low Nonspecific Binding

Yingying Liu, Tarja K. Nevanen, Arja Paananen, Kristian Kempe, Paul Wilson, Leena Sisko Johansson, Jussi J. Joensuu, Markus B. Linder, David M. Haddleton, Roberto Milani (Corresponding Author)

    Research output: Contribution to journalArticleScientificpeer-review

    20 Citations (Scopus)


    A new method is demonstrated for preparing antifouling and low nonspecific adsorption surfaces on poorly reactive hydrophobic substrates, without the need for energy-intensive or environmentally aggressive pretreatments. The surface-active protein hydrophobin was covalently modified with a controlled radical polymerization initiator and allowed to self-assemble as a monolayer on hydrophobic surfaces, followed by the preparation of antifouling surfaces by Cu(0)-mediated living radical polymerization of poly(ethylene glycol) methyl ether acrylate (PEGA) performed in situ. By taking advantage of hydrophobins to achieve at the same time the immobilization of protein A, this approach allowed to prepare surfaces for IgG1 binding featuring greatly reduced nonspecific adsorption. The success of the surface modification strategy was investigated by contact angle, XPS, and AFM characterization, while the antifouling performance and the reduction of nonspecific binding were confirmed by QCM-D measurements.
    Original languageEnglish
    Pages (from-to)3599-3608
    JournalACS Applied Materials & Interfaces
    Issue number3
    Publication statusPublished - 23 Jan 2019
    MoE publication typeNot Eligible


    This work was performed in collaboration with the Academy of Finland’s Centres of Excellence Programme (2014−2019) and is supported by the Academy of Finland (project SelfSmart, decision no. 276537 and 284508). K.K. gratefully acknowledges the award of a NHMRC-ARC Dementia Research Development Fellowship (APP1109945).


    • antifouling surfaces
    • biosensors
    • hydrophobin
    • nonspecific binding
    • surface-initiated living radical polymerization


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