Self-assembly of cellulose nanofibrils by genetically engineered fusion proteins

Suvi Varjonen, Päivi Laaksonen, Arja Paananen, Hanna Valo, Hendrik Hähl, Timo Laaksonen, Markus Linder (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

47 Citations (Scopus)

Abstract

One central problem for the function and manufacture of materials where performance relies on nanoscale structure is to control the compatibility and interactions of the building blocks. In natural materials, such as nacre, there are examples of multifunctional macromolecules that have combined binding affinities for different materials within the same molecule, thereby bridging these materials and acting as a molecular glue. Here, we describe the use of a designed multifunctional protein that is used for self-assembly of nanofibrillar cellulose. Recent advances in the production of cellulose nanofibrils have given inspiration for new uses of cellulosic materials. Cellulose nanofibrils have mechanical and structural features that open new possibilities for performance in composites and other nanoscale materials. Functionalisation was realised through a bi-functional fusion protein having both an ability to bind to cellulose and a second functionality of surface activity. The cellulose-binding function was obtained using cellulose-binding domains from cellulolytic enzymes and the surface activity through the use of a surface active protein called hydrophobin. Using the bi-functional protein, cellulose nanofibrils could be assembled into tightly packed thin films at the air/water interface and at the oil/water interface. It was shown that the combination of protein and cellulose nanofibrils resulted in a synergistic improvement in the formation and stability of oil-in-water emulsions resulting in emulsions that were stable for several months. The bi-functionality of the protein also allowed the binding of hydrophobic solid drug nanoparticles to cellulose nanofibrils and thereby improving their long-term stability under physiological conditions.
Original languageEnglish
Pages (from-to)2402-2411
Number of pages10
JournalSoft Matter
Volume7
Issue number6
DOIs
Publication statusPublished - 2011
MoE publication typeA1 Journal article-refereed

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cellulose
Cellulose
Self assembly
self assembly
Fusion reactions
fusion
proteins
Proteins
Emulsions
emulsions
Water
Nacre
Oils
oils
water
inspiration
glues
Glues
Macromolecules
macromolecules

Cite this

Varjonen, S., Laaksonen, P., Paananen, A., Valo, H., Hähl, H., Laaksonen, T., & Linder, M. (2011). Self-assembly of cellulose nanofibrils by genetically engineered fusion proteins. Soft Matter, 7(6), 2402-2411. https://doi.org/10.1039/C0SM01114B
Varjonen, Suvi ; Laaksonen, Päivi ; Paananen, Arja ; Valo, Hanna ; Hähl, Hendrik ; Laaksonen, Timo ; Linder, Markus. / Self-assembly of cellulose nanofibrils by genetically engineered fusion proteins. In: Soft Matter. 2011 ; Vol. 7, No. 6. pp. 2402-2411.
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Varjonen, S, Laaksonen, P, Paananen, A, Valo, H, Hähl, H, Laaksonen, T & Linder, M 2011, 'Self-assembly of cellulose nanofibrils by genetically engineered fusion proteins', Soft Matter, vol. 7, no. 6, pp. 2402-2411. https://doi.org/10.1039/C0SM01114B

Self-assembly of cellulose nanofibrils by genetically engineered fusion proteins. / Varjonen, Suvi; Laaksonen, Päivi; Paananen, Arja; Valo, Hanna; Hähl, Hendrik; Laaksonen, Timo; Linder, Markus (Corresponding Author).

In: Soft Matter, Vol. 7, No. 6, 2011, p. 2402-2411.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Varjonen, Suvi

AU - Laaksonen, Päivi

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AU - Valo, Hanna

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AU - Laaksonen, Timo

AU - Linder, Markus

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AB - One central problem for the function and manufacture of materials where performance relies on nanoscale structure is to control the compatibility and interactions of the building blocks. In natural materials, such as nacre, there are examples of multifunctional macromolecules that have combined binding affinities for different materials within the same molecule, thereby bridging these materials and acting as a molecular glue. Here, we describe the use of a designed multifunctional protein that is used for self-assembly of nanofibrillar cellulose. Recent advances in the production of cellulose nanofibrils have given inspiration for new uses of cellulosic materials. Cellulose nanofibrils have mechanical and structural features that open new possibilities for performance in composites and other nanoscale materials. Functionalisation was realised through a bi-functional fusion protein having both an ability to bind to cellulose and a second functionality of surface activity. The cellulose-binding function was obtained using cellulose-binding domains from cellulolytic enzymes and the surface activity through the use of a surface active protein called hydrophobin. Using the bi-functional protein, cellulose nanofibrils could be assembled into tightly packed thin films at the air/water interface and at the oil/water interface. It was shown that the combination of protein and cellulose nanofibrils resulted in a synergistic improvement in the formation and stability of oil-in-water emulsions resulting in emulsions that were stable for several months. The bi-functionality of the protein also allowed the binding of hydrophobic solid drug nanoparticles to cellulose nanofibrils and thereby improving their long-term stability under physiological conditions.

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DO - 10.1039/C0SM01114B

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JO - Soft Matter

JF - Soft Matter

SN - 1744-683X

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ER -