Self-Assembly of Soft Cellulose Nanospheres into Colloidal Gel Layers with Enhanced Protein Adsorption Capability for Next-Generation Immunoassays

Katariina Solin, Marco Beaumont (Corresponding Author), Sabine Rosenfeldt, Hannes Orelma, Maryam Borghei, Markus Bacher, Martina Opietnik, Orlando J. Rojas (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Soft cationic core/shell cellulose nanospheres can deform and interpenetrate allowing their self-assembly into densely packed colloidal nanogel layers. Taking advantage of their water-swelling capacity and molecular accessibility, the nanogels are proposed as a new and promising type of coating material to immobilize bioactive molecules on thin films and paper. The specific and nonspecific interactions between the cellulosic nanogel and human immunoglobulin G as well as bovine serum albumin (BSA) are investigated. Confocal microscopy, electroacoustic microgravimetry, and surface plasmon resonance are used to access information about the adsorption behavior and viscoelastic properties of self-assembled nanogels. A significant BSA adsorption capacity on nanogel layers (17 mg m−2) is measured, 300% higher compared to typical polymer coatings. This high protein affinity further confirms the promise of the introduced colloidal gel layer, in increasing sensitivity and advancing a new generation of substrates for a variety of applications, including immunoassays, as demonstrated in this work.

Original languageEnglish
Article number2004702
Number of pages9
JournalSmall
Volume16
Issue number50
DOIs
Publication statusPublished - 17 Dec 2020
MoE publication typeA1 Journal article-refereed

Keywords

  • amorphous nanocellulose
  • cellulose II nanogel
  • colloids
  • core/shell nanoparticles
  • immunosensors
  • protein adsorption

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