Self-Assembly of Soft Cellulose Nanospheres into Colloidal Gel Layers with Enhanced Protein Adsorption Capability for Next-Generation Immunoassays

Katariina Solin; Marco Beaumont; Sabine Rosenfeldt; Hannes Orelma; Maryam Borghei; Markus Bacher; Martina Opietnik; Orlando J. Rojas

doi:10.1002/smll.202004702

Self-Assembly of Soft Cellulose Nanospheres into Colloidal Gel Layers with Enhanced Protein Adsorption Capability for Next-Generation Immunoassays

Katariina Solin
, Marco Beaumont^*
, Sabine Rosenfeldt
, Hannes Orelma
, Maryam Borghei
, Markus Bacher
, Martina Opietnik
, Orlando J. Rojas^*

^*Corresponding author for this work

BA5407 Bioinspired materials

Aalto University
University of Natural Resources and Life Sciences (BOKU)
Bavarian Polymer Institute
Lenzing AG
University of British Columbia

Research output: Contribution to journal › Article › Scientific › peer-review

27 Citations (Scopus)

Abstract

Soft cationic core/shell cellulose nanospheres can deform and interpenetrate allowing their self-assembly into densely packed colloidal nanogel layers. Taking advantage of their water-swelling capacity and molecular accessibility, the nanogels are proposed as a new and promising type of coating material to immobilize bioactive molecules on thin films and paper. The specific and nonspecific interactions between the cellulosic nanogel and human immunoglobulin G as well as bovine serum albumin (BSA) are investigated. Confocal microscopy, electroacoustic microgravimetry, and surface plasmon resonance are used to access information about the adsorption behavior and viscoelastic properties of self-assembled nanogels. A significant BSA adsorption capacity on nanogel layers (17 mg m⁻²) is measured, 300% higher compared to typical polymer coatings. This high protein affinity further confirms the promise of the introduced colloidal gel layer, in increasing sensitivity and advancing a new generation of substrates for a variety of applications, including immunoassays, as demonstrated in this work.

Original language	English
Article number	2004702
Number of pages	9
Journal	Small
Volume	16
Issue number	50
DOIs	https://doi.org/10.1002/smll.202004702
Publication status	Published - 17 Dec 2020
MoE publication type	A1 Journal article-refereed

Funding

This project received funding from the European Union's Horizon 2020 research and innovation programme under Grant Agreement No. 760876 and the ERC Advanced Grant Agreement No. 788489, “BioElCell.” This work was also part of the Academy of Finland's Flagship Programme under Project Nos. 318890 and 318891 (Competence Center for Materials Bioeconomy, FinnCERES). K.S. acknowledges funding by the Aalto University School of Chemical Engineering doctoral programme. The Canada Excellence Research Chair initiative is gratefully acknowledged (O.J.R.).

Keywords

amorphous nanocellulose
cellulose II nanogel
colloids
core/shell nanoparticles
immunosensors
protein adsorption

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title = "Self-Assembly of Soft Cellulose Nanospheres into Colloidal Gel Layers with Enhanced Protein Adsorption Capability for Next-Generation Immunoassays",

abstract = "Soft cationic core/shell cellulose nanospheres can deform and interpenetrate allowing their self-assembly into densely packed colloidal nanogel layers. Taking advantage of their water-swelling capacity and molecular accessibility, the nanogels are proposed as a new and promising type of coating material to immobilize bioactive molecules on thin films and paper. The specific and nonspecific interactions between the cellulosic nanogel and human immunoglobulin G as well as bovine serum albumin (BSA) are investigated. Confocal microscopy, electroacoustic microgravimetry, and surface plasmon resonance are used to access information about the adsorption behavior and viscoelastic properties of self-assembled nanogels. A significant BSA adsorption capacity on nanogel layers (17 mg m−2) is measured, 300\% higher compared to typical polymer coatings. This high protein affinity further confirms the promise of the introduced colloidal gel layer, in increasing sensitivity and advancing a new generation of substrates for a variety of applications, including immunoassays, as demonstrated in this work.",

keywords = "amorphous nanocellulose, cellulose II nanogel, colloids, core/shell nanoparticles, immunosensors, protein adsorption",

author = "Katariina Solin and Marco Beaumont and Sabine Rosenfeldt and Hannes Orelma and Maryam Borghei and Markus Bacher and Martina Opietnik and Rojas, \{Orlando J.\}",

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AU - Solin, Katariina

AU - Beaumont, Marco

AU - Rosenfeldt, Sabine

AU - Orelma, Hannes

AU - Borghei, Maryam

AU - Bacher, Markus

AU - Opietnik, Martina

AU - Rojas, Orlando J.

PY - 2020/12/17

Y1 - 2020/12/17

N2 - Soft cationic core/shell cellulose nanospheres can deform and interpenetrate allowing their self-assembly into densely packed colloidal nanogel layers. Taking advantage of their water-swelling capacity and molecular accessibility, the nanogels are proposed as a new and promising type of coating material to immobilize bioactive molecules on thin films and paper. The specific and nonspecific interactions between the cellulosic nanogel and human immunoglobulin G as well as bovine serum albumin (BSA) are investigated. Confocal microscopy, electroacoustic microgravimetry, and surface plasmon resonance are used to access information about the adsorption behavior and viscoelastic properties of self-assembled nanogels. A significant BSA adsorption capacity on nanogel layers (17 mg m−2) is measured, 300% higher compared to typical polymer coatings. This high protein affinity further confirms the promise of the introduced colloidal gel layer, in increasing sensitivity and advancing a new generation of substrates for a variety of applications, including immunoassays, as demonstrated in this work.

AB - Soft cationic core/shell cellulose nanospheres can deform and interpenetrate allowing their self-assembly into densely packed colloidal nanogel layers. Taking advantage of their water-swelling capacity and molecular accessibility, the nanogels are proposed as a new and promising type of coating material to immobilize bioactive molecules on thin films and paper. The specific and nonspecific interactions between the cellulosic nanogel and human immunoglobulin G as well as bovine serum albumin (BSA) are investigated. Confocal microscopy, electroacoustic microgravimetry, and surface plasmon resonance are used to access information about the adsorption behavior and viscoelastic properties of self-assembled nanogels. A significant BSA adsorption capacity on nanogel layers (17 mg m−2) is measured, 300% higher compared to typical polymer coatings. This high protein affinity further confirms the promise of the introduced colloidal gel layer, in increasing sensitivity and advancing a new generation of substrates for a variety of applications, including immunoassays, as demonstrated in this work.

KW - amorphous nanocellulose

KW - cellulose II nanogel

KW - colloids

KW - core/shell nanoparticles

KW - immunosensors

KW - protein adsorption

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Self-Assembly of Soft Cellulose Nanospheres into Colloidal Gel Layers with Enhanced Protein Adsorption Capability for Next-Generation Immunoassays

Abstract

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Keywords

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