Separation of catecholamines with capillary electrophoresis by using dynamic and static coatings

Siren, Stella Rovio, Kaija Luomanperä, Pertti Vastamäki, Teemu Työppönen, Lotta Salminen, Pekka Savolahti

Research output: Contribution to conferenceConference PosterScientific

Abstract

The studies have been focused on to improve the separation of catecholamines with capillary electrophoresis by using dynamic and static coatings. The results are used in the analyses of drugs from biological fluids with m-chips. Adrenaline, dopamine and noradrenaline are neurotransmitters in the central and peripherial nervous systems. Their determination, together with the compounds formed in their chemical reactions occurring within living organisms, is useful for clinical diagnosis of pheochromocytoma, neuroblastoma and Parkinson s disease. Different analytical methods like HRGC, HPLC and CE have been used for their analyses in urine and blood samples. Capillary electrophoresis (CE) with UV-detection has shown its potential in the determination of catecholamines from urine. When fused silica capillary is used, in-run modification can be formed by positively charged organic molecules, which can adsorb on the negatively charged silanol groups. Therefore covalent bondings are not formed. The reagents, which adsorb on the silica wall, are used as additives in the electrolyte solution, and thus they can be actively changed with fresh molecules. The commonly used modifiers for in-run modification have been e.g. ethyleneamine, different salts acting as zwitterions or triethylamine.
Original languageEnglish
Publication statusPublished - 2003
EventVTT Life Science Day - Espoo, Finland
Duration: 8 May 20038 May 2003

Seminar

SeminarVTT Life Science Day
CountryFinland
CityEspoo
Period8/05/038/05/03

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Capillary electrophoresis
Catecholamines
Coatings
Molecules
Neurology
Fused silica
Silicon Dioxide
Electrolytes
Epinephrine
Neurotransmitter Agents
Chemical reactions
Dopamine
Norepinephrine
Blood
Salts
Fluids
Pharmaceutical Preparations

Cite this

Siren, Rovio, S., Luomanperä, K., Vastamäki, P., Työppönen, T., Salminen, L., & Savolahti, P. (2003). Separation of catecholamines with capillary electrophoresis by using dynamic and static coatings. Poster session presented at VTT Life Science Day, Espoo, Finland.
Siren ; Rovio, Stella ; Luomanperä, Kaija ; Vastamäki, Pertti ; Työppönen, Teemu ; Salminen, Lotta ; Savolahti, Pekka. / Separation of catecholamines with capillary electrophoresis by using dynamic and static coatings. Poster session presented at VTT Life Science Day, Espoo, Finland.
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Siren, Rovio, S, Luomanperä, K, Vastamäki, P, Työppönen, T, Salminen, L & Savolahti, P 2003, 'Separation of catecholamines with capillary electrophoresis by using dynamic and static coatings' VTT Life Science Day, Espoo, Finland, 8/05/03 - 8/05/03, .

Separation of catecholamines with capillary electrophoresis by using dynamic and static coatings. / Siren; Rovio, Stella; Luomanperä, Kaija; Vastamäki, Pertti; Työppönen, Teemu; Salminen, Lotta; Savolahti, Pekka.

2003. Poster session presented at VTT Life Science Day, Espoo, Finland.

Research output: Contribution to conferenceConference PosterScientific

TY - CONF

T1 - Separation of catecholamines with capillary electrophoresis by using dynamic and static coatings

AU - Siren, null

AU - Rovio, Stella

AU - Luomanperä, Kaija

AU - Vastamäki, Pertti

AU - Työppönen, Teemu

AU - Salminen, Lotta

AU - Savolahti, Pekka

PY - 2003

Y1 - 2003

N2 - The studies have been focused on to improve the separation of catecholamines with capillary electrophoresis by using dynamic and static coatings. The results are used in the analyses of drugs from biological fluids with m-chips. Adrenaline, dopamine and noradrenaline are neurotransmitters in the central and peripherial nervous systems. Their determination, together with the compounds formed in their chemical reactions occurring within living organisms, is useful for clinical diagnosis of pheochromocytoma, neuroblastoma and Parkinson s disease. Different analytical methods like HRGC, HPLC and CE have been used for their analyses in urine and blood samples. Capillary electrophoresis (CE) with UV-detection has shown its potential in the determination of catecholamines from urine. When fused silica capillary is used, in-run modification can be formed by positively charged organic molecules, which can adsorb on the negatively charged silanol groups. Therefore covalent bondings are not formed. The reagents, which adsorb on the silica wall, are used as additives in the electrolyte solution, and thus they can be actively changed with fresh molecules. The commonly used modifiers for in-run modification have been e.g. ethyleneamine, different salts acting as zwitterions or triethylamine.

AB - The studies have been focused on to improve the separation of catecholamines with capillary electrophoresis by using dynamic and static coatings. The results are used in the analyses of drugs from biological fluids with m-chips. Adrenaline, dopamine and noradrenaline are neurotransmitters in the central and peripherial nervous systems. Their determination, together with the compounds formed in their chemical reactions occurring within living organisms, is useful for clinical diagnosis of pheochromocytoma, neuroblastoma and Parkinson s disease. Different analytical methods like HRGC, HPLC and CE have been used for their analyses in urine and blood samples. Capillary electrophoresis (CE) with UV-detection has shown its potential in the determination of catecholamines from urine. When fused silica capillary is used, in-run modification can be formed by positively charged organic molecules, which can adsorb on the negatively charged silanol groups. Therefore covalent bondings are not formed. The reagents, which adsorb on the silica wall, are used as additives in the electrolyte solution, and thus they can be actively changed with fresh molecules. The commonly used modifiers for in-run modification have been e.g. ethyleneamine, different salts acting as zwitterions or triethylamine.

M3 - Conference Poster

ER -

Siren, Rovio S, Luomanperä K, Vastamäki P, Työppönen T, Salminen L et al. Separation of catecholamines with capillary electrophoresis by using dynamic and static coatings. 2003. Poster session presented at VTT Life Science Day, Espoo, Finland.