Separation of enzymes produced by Trichoderma reesei with hydrophobic ultrafiltration membranes

Neda Ehsani; Marianne Nyström; Heikki Ojamo; Matti Siika-aho

doi:10.1016/0032-9592(95)00057-7

Separation of enzymes produced by Trichoderma reesei with hydrophobic ultrafiltration membranes

Neda Ehsani, Marianne Nyström (Corresponding Author), Heikki Ojamo, Matti Siika-aho

Lappeenranta-Lahti University of Technology LUT

Research output: Contribution to journal › Article › Scientific › peer-review

9 Citations (Scopus)

Abstract

The separation of a β-xylanase by ultrafiltration from a mixture of xylanases and cellulases produced by Trichoderma reesei was investigated. The effects of the sizes and the pH dependent charges of the proteins and the membranes on separation were studied. A stirred cell device with hydrophobic polysulphone membranes of different cut-off values and manufacturers was employed and there were distinct differences in the pore charges of the virgin membranes from different manufacturers. The flux reductions for the membranes were high and the permeate fluxes dropped to the same flux level in spite of the large differences in the initial water fluxes. β-Xylanase (isoelectric point 9·0) selectivity increased with increasing pH and high selectivity values were obtained with PM30 membranes at pH 7·0.

Original language	English
Pages (from-to)	253-263
Number of pages	11
Journal	Process Biochemistry
Volume	31
Issue number	3
DOIs	https://doi.org/10.1016/0032-9592(95)00057-7
Publication status	Published - 1996
MoE publication type	A1 Journal article-refereed

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title = "Separation of enzymes produced by Trichoderma reesei with hydrophobic ultrafiltration membranes",

abstract = "The separation of a β-xylanase by ultrafiltration from a mixture of xylanases and cellulases produced by Trichoderma reesei was investigated. The effects of the sizes and the pH dependent charges of the proteins and the membranes on separation were studied. A stirred cell device with hydrophobic polysulphone membranes of different cut-off values and manufacturers was employed and there were distinct differences in the pore charges of the virgin membranes from different manufacturers. The flux reductions for the membranes were high and the permeate fluxes dropped to the same flux level in spite of the large differences in the initial water fluxes. β-Xylanase (isoelectric point 9·0) selectivity increased with increasing pH and high selectivity values were obtained with PM30 membranes at pH 7·0.",

author = "Neda Ehsani and Marianne Nystr{\"o}m and Heikki Ojamo and Matti Siika-aho",

note = "Project code: B6SU00067",

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journal = "Process Biochemistry",

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T1 - Separation of enzymes produced by Trichoderma reesei with hydrophobic ultrafiltration membranes

AU - Ehsani, Neda

AU - Nyström, Marianne

AU - Ojamo, Heikki

AU - Siika-aho, Matti

N1 - Project code: B6SU00067

PY - 1996

Y1 - 1996

N2 - The separation of a β-xylanase by ultrafiltration from a mixture of xylanases and cellulases produced by Trichoderma reesei was investigated. The effects of the sizes and the pH dependent charges of the proteins and the membranes on separation were studied. A stirred cell device with hydrophobic polysulphone membranes of different cut-off values and manufacturers was employed and there were distinct differences in the pore charges of the virgin membranes from different manufacturers. The flux reductions for the membranes were high and the permeate fluxes dropped to the same flux level in spite of the large differences in the initial water fluxes. β-Xylanase (isoelectric point 9·0) selectivity increased with increasing pH and high selectivity values were obtained with PM30 membranes at pH 7·0.

AB - The separation of a β-xylanase by ultrafiltration from a mixture of xylanases and cellulases produced by Trichoderma reesei was investigated. The effects of the sizes and the pH dependent charges of the proteins and the membranes on separation were studied. A stirred cell device with hydrophobic polysulphone membranes of different cut-off values and manufacturers was employed and there were distinct differences in the pore charges of the virgin membranes from different manufacturers. The flux reductions for the membranes were high and the permeate fluxes dropped to the same flux level in spite of the large differences in the initial water fluxes. β-Xylanase (isoelectric point 9·0) selectivity increased with increasing pH and high selectivity values were obtained with PM30 membranes at pH 7·0.

U2 - 10.1016/0032-9592(95)00057-7

DO - 10.1016/0032-9592(95)00057-7

M3 - Article

SN - 1359-5113

VL - 31

SP - 253

EP - 263

JO - Process Biochemistry

JF - Process Biochemistry

IS - 3

ER -

Separation of enzymes produced by Trichoderma reesei with hydrophobic ultrafiltration membranes

Abstract

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