Separation of enzymes produced by Trichoderma reesei with hydrophobic ultrafiltration membranes

Neda Ehsani, Marianne Nyström (Corresponding Author), Heikki Ojamo, Matti Siika-aho

Research output: Contribution to journalArticleScientificpeer-review

9 Citations (Scopus)

Abstract

The separation of a β-xylanase by ultrafiltration from a mixture of xylanases and cellulases produced by Trichoderma reesei was investigated. The effects of the sizes and the pH dependent charges of the proteins and the membranes on separation were studied. A stirred cell device with hydrophobic polysulphone membranes of different cut-off values and manufacturers was employed and there were distinct differences in the pore charges of the virgin membranes from different manufacturers. The flux reductions for the membranes were high and the permeate fluxes dropped to the same flux level in spite of the large differences in the initial water fluxes. β-Xylanase (isoelectric point 9·0) selectivity increased with increasing pH and high selectivity values were obtained with PM30 membranes at pH 7·0.
Original languageEnglish
Pages (from-to)253-263
Number of pages11
JournalProcess Biochemistry
Volume31
Issue number3
DOIs
Publication statusPublished - 1996
MoE publication typeA1 Journal article-refereed

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Trichoderma
Ultrafiltration
Enzymes
Fluxes
Membranes
Cellulases
Isoelectric Point
Polysulfones
Membrane Proteins
Equipment and Supplies
Water
Proteins

Cite this

Ehsani, Neda ; Nyström, Marianne ; Ojamo, Heikki ; Siika-aho, Matti. / Separation of enzymes produced by Trichoderma reesei with hydrophobic ultrafiltration membranes. In: Process Biochemistry. 1996 ; Vol. 31, No. 3. pp. 253-263.
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abstract = "The separation of a β-xylanase by ultrafiltration from a mixture of xylanases and cellulases produced by Trichoderma reesei was investigated. The effects of the sizes and the pH dependent charges of the proteins and the membranes on separation were studied. A stirred cell device with hydrophobic polysulphone membranes of different cut-off values and manufacturers was employed and there were distinct differences in the pore charges of the virgin membranes from different manufacturers. The flux reductions for the membranes were high and the permeate fluxes dropped to the same flux level in spite of the large differences in the initial water fluxes. β-Xylanase (isoelectric point 9·0) selectivity increased with increasing pH and high selectivity values were obtained with PM30 membranes at pH 7·0.",
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Separation of enzymes produced by Trichoderma reesei with hydrophobic ultrafiltration membranes. / Ehsani, Neda; Nyström, Marianne (Corresponding Author); Ojamo, Heikki; Siika-aho, Matti.

In: Process Biochemistry, Vol. 31, No. 3, 1996, p. 253-263.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Separation of enzymes produced by Trichoderma reesei with hydrophobic ultrafiltration membranes

AU - Ehsani, Neda

AU - Nyström, Marianne

AU - Ojamo, Heikki

AU - Siika-aho, Matti

N1 - Project code: B6SU00067

PY - 1996

Y1 - 1996

N2 - The separation of a β-xylanase by ultrafiltration from a mixture of xylanases and cellulases produced by Trichoderma reesei was investigated. The effects of the sizes and the pH dependent charges of the proteins and the membranes on separation were studied. A stirred cell device with hydrophobic polysulphone membranes of different cut-off values and manufacturers was employed and there were distinct differences in the pore charges of the virgin membranes from different manufacturers. The flux reductions for the membranes were high and the permeate fluxes dropped to the same flux level in spite of the large differences in the initial water fluxes. β-Xylanase (isoelectric point 9·0) selectivity increased with increasing pH and high selectivity values were obtained with PM30 membranes at pH 7·0.

AB - The separation of a β-xylanase by ultrafiltration from a mixture of xylanases and cellulases produced by Trichoderma reesei was investigated. The effects of the sizes and the pH dependent charges of the proteins and the membranes on separation were studied. A stirred cell device with hydrophobic polysulphone membranes of different cut-off values and manufacturers was employed and there were distinct differences in the pore charges of the virgin membranes from different manufacturers. The flux reductions for the membranes were high and the permeate fluxes dropped to the same flux level in spite of the large differences in the initial water fluxes. β-Xylanase (isoelectric point 9·0) selectivity increased with increasing pH and high selectivity values were obtained with PM30 membranes at pH 7·0.

U2 - 10.1016/0032-9592(95)00057-7

DO - 10.1016/0032-9592(95)00057-7

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EP - 263

JO - Process Biochemistry

JF - Process Biochemistry

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