Separation of enzymes produced by Trichoderma reesei with hydrophobic ultrafiltration membranes

Neda Ehsani, Marianne Nyström (Corresponding Author), Heikki Ojamo, Matti Siika-aho

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    9 Citations (Scopus)

    Abstract

    The separation of a β-xylanase by ultrafiltration from a mixture of xylanases and cellulases produced by Trichoderma reesei was investigated. The effects of the sizes and the pH dependent charges of the proteins and the membranes on separation were studied. A stirred cell device with hydrophobic polysulphone membranes of different cut-off values and manufacturers was employed and there were distinct differences in the pore charges of the virgin membranes from different manufacturers. The flux reductions for the membranes were high and the permeate fluxes dropped to the same flux level in spite of the large differences in the initial water fluxes. β-Xylanase (isoelectric point 9·0) selectivity increased with increasing pH and high selectivity values were obtained with PM30 membranes at pH 7·0.
    Original languageEnglish
    Pages (from-to)253-263
    Number of pages11
    JournalProcess Biochemistry
    Volume31
    Issue number3
    DOIs
    Publication statusPublished - 1996
    MoE publication typeA1 Journal article-refereed

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