Separation, purity testing and identification of cyanobacterial hepatotoxins with capillary electrophoresis and electrospray mass spectrometry

Heli Siren (Corresponding Author), Matti Jussila, Huwei Liu, Sanna Peltoniemi, Kaarina Sivonen, Marja-Liisa Riekkola

Research output: Contribution to journalArticleScientificpeer-review

33 Citations (Scopus)

Abstract

Capillary zone electrophoretic and micellar electrokinetic chromatographic methods with UV detection were investigated for separation and identification of some microcystins isolated from cyanobacterial Anabaena 90 strains in Finland. The low-sensitivity purity tests with full scan UV–Vis spectra were done with a CZE method. All the test compounds, [d-Asp3,Dha7]MCYST-LR, MCYST-LR, MCYST-YR, [Dha7]MCYST-LR, MCYST-RR, [Dha7]MCYST-RR, [d-Asp3,Dha7]MCYST-RR, [d-Asp3]MCYST-LR and [d-Asp3]MCYST-RR (where MCYST stands for microcystin) isolated with a preparative HPLC method, could be separated from each other in a MECC method. The detection limits of the microcystins were below ppm level. The repeatability of the MECC technique was tested by comparing the absolute migration times with the indices calculated with “in-laboratory” designed programs operating in MATLAB Mathworks Inc. The identification and the high-sensitivity purity tests of the isolated microcystins were made with an off-line electrospray mass spectrometer. Identification could be done for MCYST-YR, MCYST-LR, MCYST-RR, [Dha7]MCYST-RR, [Dha7]MCYST-RR, [d-Asp3]MCYST-LR, [d-Asp3]MCYST-RR, [d-Asp3, Dha7]MCYST-LR and [d-Asp3, Dha7]MCYST-RR on the basis of their [M+H]+ or [M+2H]2+ ions in the MS mode and by using the protonated molecule as precursor in the MS–MS mode.
Original languageEnglish
Pages (from-to)203-215
Number of pages13
JournalJournal of Chromatography A
Volume839
Issue number1-2
DOIs
Publication statusPublished - 1999
MoE publication typeA1 Journal article-refereed

Fingerprint

Capillary electrophoresis
Capillary Electrophoresis
Mass spectrometry
Mass Spectrometry
Microcystins
Testing
Micellar Electrokinetic Capillary Chromatography
Anabaena
Mass spectrometers
Finland
cyanoginosin LR
MATLAB
Limit of Detection
High Pressure Liquid Chromatography
Ions
Molecules

Cite this

Siren, Heli ; Jussila, Matti ; Liu, Huwei ; Peltoniemi, Sanna ; Sivonen, Kaarina ; Riekkola, Marja-Liisa. / Separation, purity testing and identification of cyanobacterial hepatotoxins with capillary electrophoresis and electrospray mass spectrometry. In: Journal of Chromatography A. 1999 ; Vol. 839, No. 1-2. pp. 203-215.
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title = "Separation, purity testing and identification of cyanobacterial hepatotoxins with capillary electrophoresis and electrospray mass spectrometry",
abstract = "Capillary zone electrophoretic and micellar electrokinetic chromatographic methods with UV detection were investigated for separation and identification of some microcystins isolated from cyanobacterial Anabaena 90 strains in Finland. The low-sensitivity purity tests with full scan UV–Vis spectra were done with a CZE method. All the test compounds, [d-Asp3,Dha7]MCYST-LR, MCYST-LR, MCYST-YR, [Dha7]MCYST-LR, MCYST-RR, [Dha7]MCYST-RR, [d-Asp3,Dha7]MCYST-RR, [d-Asp3]MCYST-LR and [d-Asp3]MCYST-RR (where MCYST stands for microcystin) isolated with a preparative HPLC method, could be separated from each other in a MECC method. The detection limits of the microcystins were below ppm level. The repeatability of the MECC technique was tested by comparing the absolute migration times with the indices calculated with “in-laboratory” designed programs operating in MATLAB Mathworks Inc. The identification and the high-sensitivity purity tests of the isolated microcystins were made with an off-line electrospray mass spectrometer. Identification could be done for MCYST-YR, MCYST-LR, MCYST-RR, [Dha7]MCYST-RR, [Dha7]MCYST-RR, [d-Asp3]MCYST-LR, [d-Asp3]MCYST-RR, [d-Asp3, Dha7]MCYST-LR and [d-Asp3, Dha7]MCYST-RR on the basis of their [M+H]+ or [M+2H]2+ ions in the MS mode and by using the protonated molecule as precursor in the MS–MS mode.",
author = "Heli Siren and Matti Jussila and Huwei Liu and Sanna Peltoniemi and Kaarina Sivonen and Marja-Liisa Riekkola",
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Separation, purity testing and identification of cyanobacterial hepatotoxins with capillary electrophoresis and electrospray mass spectrometry. / Siren, Heli (Corresponding Author); Jussila, Matti; Liu, Huwei; Peltoniemi, Sanna; Sivonen, Kaarina; Riekkola, Marja-Liisa.

In: Journal of Chromatography A, Vol. 839, No. 1-2, 1999, p. 203-215.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Separation, purity testing and identification of cyanobacterial hepatotoxins with capillary electrophoresis and electrospray mass spectrometry

AU - Siren, Heli

AU - Jussila, Matti

AU - Liu, Huwei

AU - Peltoniemi, Sanna

AU - Sivonen, Kaarina

AU - Riekkola, Marja-Liisa

PY - 1999

Y1 - 1999

N2 - Capillary zone electrophoretic and micellar electrokinetic chromatographic methods with UV detection were investigated for separation and identification of some microcystins isolated from cyanobacterial Anabaena 90 strains in Finland. The low-sensitivity purity tests with full scan UV–Vis spectra were done with a CZE method. All the test compounds, [d-Asp3,Dha7]MCYST-LR, MCYST-LR, MCYST-YR, [Dha7]MCYST-LR, MCYST-RR, [Dha7]MCYST-RR, [d-Asp3,Dha7]MCYST-RR, [d-Asp3]MCYST-LR and [d-Asp3]MCYST-RR (where MCYST stands for microcystin) isolated with a preparative HPLC method, could be separated from each other in a MECC method. The detection limits of the microcystins were below ppm level. The repeatability of the MECC technique was tested by comparing the absolute migration times with the indices calculated with “in-laboratory” designed programs operating in MATLAB Mathworks Inc. The identification and the high-sensitivity purity tests of the isolated microcystins were made with an off-line electrospray mass spectrometer. Identification could be done for MCYST-YR, MCYST-LR, MCYST-RR, [Dha7]MCYST-RR, [Dha7]MCYST-RR, [d-Asp3]MCYST-LR, [d-Asp3]MCYST-RR, [d-Asp3, Dha7]MCYST-LR and [d-Asp3, Dha7]MCYST-RR on the basis of their [M+H]+ or [M+2H]2+ ions in the MS mode and by using the protonated molecule as precursor in the MS–MS mode.

AB - Capillary zone electrophoretic and micellar electrokinetic chromatographic methods with UV detection were investigated for separation and identification of some microcystins isolated from cyanobacterial Anabaena 90 strains in Finland. The low-sensitivity purity tests with full scan UV–Vis spectra were done with a CZE method. All the test compounds, [d-Asp3,Dha7]MCYST-LR, MCYST-LR, MCYST-YR, [Dha7]MCYST-LR, MCYST-RR, [Dha7]MCYST-RR, [d-Asp3,Dha7]MCYST-RR, [d-Asp3]MCYST-LR and [d-Asp3]MCYST-RR (where MCYST stands for microcystin) isolated with a preparative HPLC method, could be separated from each other in a MECC method. The detection limits of the microcystins were below ppm level. The repeatability of the MECC technique was tested by comparing the absolute migration times with the indices calculated with “in-laboratory” designed programs operating in MATLAB Mathworks Inc. The identification and the high-sensitivity purity tests of the isolated microcystins were made with an off-line electrospray mass spectrometer. Identification could be done for MCYST-YR, MCYST-LR, MCYST-RR, [Dha7]MCYST-RR, [Dha7]MCYST-RR, [d-Asp3]MCYST-LR, [d-Asp3]MCYST-RR, [d-Asp3, Dha7]MCYST-LR and [d-Asp3, Dha7]MCYST-RR on the basis of their [M+H]+ or [M+2H]2+ ions in the MS mode and by using the protonated molecule as precursor in the MS–MS mode.

U2 - 10.1016/S0021-9673(99)00105-3

DO - 10.1016/S0021-9673(99)00105-3

M3 - Article

VL - 839

SP - 203

EP - 215

JO - Journal of Chromatography A

JF - Journal of Chromatography A

SN - 0021-9673

IS - 1-2

ER -