Abstract
In order to assist developing a natural, safe food-preservative, aqueous ethanolic extracts of leaves and berries of eight Finnish berry plants were fractionated with Sephadex LH-20 column chromatography. For each fraction, phenolic compounds were analyzed with NMR, UPLC-DAD-ESI-MS and HPLC-DAD. The antioxidant activities of the fractions were investigated using oxygen radical absorbance capacity (ORAC) assay, and the antibacterial activities were evaluated against foodborne pathogens Staphylococcus aureus and Escherichia coli. Antioxidant activities of the fractions correlated highly with both the total concentration and structural feature of phenolic compounds, including both flavonoids and non-flavonoid phenolics. ORAC value correlated strongly with the concentration of (+)-catechin, (−)-epicatecin, quercetin glycosides, and anthocyanins. Increase in size and number of sugar moieties may reduce the antioxidative activities of quercetin glycosides. Type of sugar moieties may have a significant role in influencing peroxyl-radicals scavenging ability of quercetin glycosides with monosaccharides as a single sugar moieties. Most of the fractions inhibited the target microbes. S. aureus strains expressed a higher sensitivity to phenolic compounds than E. coli strains.
| Original language | English |
|---|---|
| Pages (from-to) | 115-130 |
| Number of pages | 16 |
| Journal | Food Research International |
| Volume | 113 |
| DOIs | |
| Publication status | Published - 1 Nov 2018 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- Anti-bacteria
- Antioxidant
- Berry
- Leaf
- Phenolic compounds
- Sephadex LH-20
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Sephadex LH-20 fractionation and bioactivities of phenolic compounds from extracts of Finnish berry plants. / Tian, Ye; Liimatainen, Jaana; Puganen, Anna; Alakomi, Hanna Leena; Sinkkonen, Jari; Yang, Baoru (Corresponding Author).
In: Food Research International, Vol. 113, 01.11.2018, p. 115-130.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Sephadex LH-20 fractionation and bioactivities of phenolic compounds from extracts of Finnish berry plants
AU - Tian, Ye
AU - Liimatainen, Jaana
AU - Puganen, Anna
AU - Alakomi, Hanna Leena
AU - Sinkkonen, Jari
AU - Yang, Baoru
PY - 2018/11/1
Y1 - 2018/11/1
N2 - In order to assist developing a natural, safe food-preservative, aqueous ethanolic extracts of leaves and berries of eight Finnish berry plants were fractionated with Sephadex LH-20 column chromatography. For each fraction, phenolic compounds were analyzed with NMR, UPLC-DAD-ESI-MS and HPLC-DAD. The antioxidant activities of the fractions were investigated using oxygen radical absorbance capacity (ORAC) assay, and the antibacterial activities were evaluated against foodborne pathogens Staphylococcus aureus and Escherichia coli. Antioxidant activities of the fractions correlated highly with both the total concentration and structural feature of phenolic compounds, including both flavonoids and non-flavonoid phenolics. ORAC value correlated strongly with the concentration of (+)-catechin, (−)-epicatecin, quercetin glycosides, and anthocyanins. Increase in size and number of sugar moieties may reduce the antioxidative activities of quercetin glycosides. Type of sugar moieties may have a significant role in influencing peroxyl-radicals scavenging ability of quercetin glycosides with monosaccharides as a single sugar moieties. Most of the fractions inhibited the target microbes. S. aureus strains expressed a higher sensitivity to phenolic compounds than E. coli strains.
AB - In order to assist developing a natural, safe food-preservative, aqueous ethanolic extracts of leaves and berries of eight Finnish berry plants were fractionated with Sephadex LH-20 column chromatography. For each fraction, phenolic compounds were analyzed with NMR, UPLC-DAD-ESI-MS and HPLC-DAD. The antioxidant activities of the fractions were investigated using oxygen radical absorbance capacity (ORAC) assay, and the antibacterial activities were evaluated against foodborne pathogens Staphylococcus aureus and Escherichia coli. Antioxidant activities of the fractions correlated highly with both the total concentration and structural feature of phenolic compounds, including both flavonoids and non-flavonoid phenolics. ORAC value correlated strongly with the concentration of (+)-catechin, (−)-epicatecin, quercetin glycosides, and anthocyanins. Increase in size and number of sugar moieties may reduce the antioxidative activities of quercetin glycosides. Type of sugar moieties may have a significant role in influencing peroxyl-radicals scavenging ability of quercetin glycosides with monosaccharides as a single sugar moieties. Most of the fractions inhibited the target microbes. S. aureus strains expressed a higher sensitivity to phenolic compounds than E. coli strains.
KW - Anti-bacteria
KW - Antioxidant
KW - Berry
KW - Leaf
KW - Phenolic compounds
KW - Sephadex LH-20
UR - http://www.scopus.com/inward/record.url?scp=85049449397&partnerID=8YFLogxK
U2 - 10.1016/j.foodres.2018.06.041
DO - 10.1016/j.foodres.2018.06.041
M3 - Article
AN - SCOPUS:85049449397
VL - 113
SP - 115
EP - 130
JO - Food Research International
JF - Food Research International
SN - 0963-9969
ER -