Sephadex LH-20 fractionation and bioactivities of phenolic compounds from extracts of Finnish berry plants

Ye Tian, Jaana Liimatainen, Anna Puganen, Hanna Leena Alakomi, Jari Sinkkonen, Baoru Yang (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

1 Citation (Scopus)

Abstract

In order to assist developing a natural, safe food-preservative, aqueous ethanolic extracts of leaves and berries of eight Finnish berry plants were fractionated with Sephadex LH-20 column chromatography. For each fraction, phenolic compounds were analyzed with NMR, UPLC-DAD-ESI-MS and HPLC-DAD. The antioxidant activities of the fractions were investigated using oxygen radical absorbance capacity (ORAC) assay, and the antibacterial activities were evaluated against foodborne pathogens Staphylococcus aureus and Escherichia coli. Antioxidant activities of the fractions correlated highly with both the total concentration and structural feature of phenolic compounds, including both flavonoids and non-flavonoid phenolics. ORAC value correlated strongly with the concentration of (+)-catechin, (−)-epicatecin, quercetin glycosides, and anthocyanins. Increase in size and number of sugar moieties may reduce the antioxidative activities of quercetin glycosides. Type of sugar moieties may have a significant role in influencing peroxyl-radicals scavenging ability of quercetin glycosides with monosaccharides as a single sugar moieties. Most of the fractions inhibited the target microbes. S. aureus strains expressed a higher sensitivity to phenolic compounds than E. coli strains.

Original languageEnglish
Pages (from-to)115-130
Number of pages16
JournalFood Research International
Volume113
DOIs
Publication statusPublished - 1 Nov 2018
MoE publication typeA1 Journal article-refereed

Fingerprint

Quercetin
Glycosides
quercetin
small fruits
glycosides
phenolic compounds
Fruit
fractionation
oxygen radical absorbance capacity
antioxidant activity
sugars
Staphylococcus aureus
extracts
Antioxidants
Food Preservatives
Escherichia coli
food preservatives
Anthocyanins
Catechin
Monosaccharides

Keywords

  • Anti-bacteria
  • Antioxidant
  • Berry
  • Leaf
  • Phenolic compounds
  • Sephadex LH-20

Cite this

Tian, Ye ; Liimatainen, Jaana ; Puganen, Anna ; Alakomi, Hanna Leena ; Sinkkonen, Jari ; Yang, Baoru. / Sephadex LH-20 fractionation and bioactivities of phenolic compounds from extracts of Finnish berry plants. In: Food Research International. 2018 ; Vol. 113. pp. 115-130.
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abstract = "In order to assist developing a natural, safe food-preservative, aqueous ethanolic extracts of leaves and berries of eight Finnish berry plants were fractionated with Sephadex LH-20 column chromatography. For each fraction, phenolic compounds were analyzed with NMR, UPLC-DAD-ESI-MS and HPLC-DAD. The antioxidant activities of the fractions were investigated using oxygen radical absorbance capacity (ORAC) assay, and the antibacterial activities were evaluated against foodborne pathogens Staphylococcus aureus and Escherichia coli. Antioxidant activities of the fractions correlated highly with both the total concentration and structural feature of phenolic compounds, including both flavonoids and non-flavonoid phenolics. ORAC value correlated strongly with the concentration of (+)-catechin, (−)-epicatecin, quercetin glycosides, and anthocyanins. Increase in size and number of sugar moieties may reduce the antioxidative activities of quercetin glycosides. Type of sugar moieties may have a significant role in influencing peroxyl-radicals scavenging ability of quercetin glycosides with monosaccharides as a single sugar moieties. Most of the fractions inhibited the target microbes. S. aureus strains expressed a higher sensitivity to phenolic compounds than E. coli strains.",
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Sephadex LH-20 fractionation and bioactivities of phenolic compounds from extracts of Finnish berry plants. / Tian, Ye; Liimatainen, Jaana; Puganen, Anna; Alakomi, Hanna Leena; Sinkkonen, Jari; Yang, Baoru (Corresponding Author).

In: Food Research International, Vol. 113, 01.11.2018, p. 115-130.

Research output: Contribution to journalArticleScientificpeer-review

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