Sequestration of N-acetyl-β-D-glucosaminidase in somatic cells during experimental bovine mastitis induced by endotoxin, Staphylococcus aureus or Streptococcus agalactiae

Liisa Kaartinen, Tiina Mattila, Alan Frost, Markus Sandholm

Research output: Contribution to journalArticleScientificpeer-review

7 Citations (Scopus)

Abstract

Experimental mastitis was induced in cows by intramammary infusion of endotoxin, Staphylococcus aureus or Streptococcus agalactiae. The inflammatory response was monitored by somatic cell counting and N-acetyl-β-D-glucosaminidase (NAGase). NAGase activity was analysed in fresh milk samples in parallel with samples treated by a cycle of freezing and thawing combined with detergent treatment to release the cell-bound NAGase. Before the udder reacted by inflammation, the total NAGase activity consisted of free extracellular activity. Later on when the inflammation was established, much of the milk NAGase remained sequestered intracellularly. S agalactiae was linked with a high degree of cellular NAGase sequestration indicating a blockage of the lysosomal release function from the phagocytes. S aureus delayed the inflammatory response.
Original languageEnglish
Pages (from-to)306-309
JournalResearch in Veterinary Science
Volume48
Issue number3
DOIs
Publication statusPublished - 1990
MoE publication typeA1 Journal article-refereed

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Bovine Mastitis
Hexosaminidases
Streptococcus agalactiae
bovine mastitis
endotoxins
somatic cells
Endotoxins
Staphylococcus aureus
inflammation
milk
phagocytes
Milk
udders
thawing
detergents
Inflammation
mastitis
Animal Mammary Glands
freezing
Mastitis

Cite this

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title = "Sequestration of N-acetyl-β-D-glucosaminidase in somatic cells during experimental bovine mastitis induced by endotoxin, Staphylococcus aureus or Streptococcus agalactiae",
abstract = "Experimental mastitis was induced in cows by intramammary infusion of endotoxin, Staphylococcus aureus or Streptococcus agalactiae. The inflammatory response was monitored by somatic cell counting and N-acetyl-β-D-glucosaminidase (NAGase). NAGase activity was analysed in fresh milk samples in parallel with samples treated by a cycle of freezing and thawing combined with detergent treatment to release the cell-bound NAGase. Before the udder reacted by inflammation, the total NAGase activity consisted of free extracellular activity. Later on when the inflammation was established, much of the milk NAGase remained sequestered intracellularly. S agalactiae was linked with a high degree of cellular NAGase sequestration indicating a blockage of the lysosomal release function from the phagocytes. S aureus delayed the inflammatory response.",
author = "Liisa Kaartinen and Tiina Mattila and Alan Frost and Markus Sandholm",
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doi = "10.1016/S0034-5288(18)31018-X",
language = "English",
volume = "48",
pages = "306--309",
journal = "Research in Veterinary Science",
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publisher = "Elsevier",
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Sequestration of N-acetyl-β-D-glucosaminidase in somatic cells during experimental bovine mastitis induced by endotoxin, Staphylococcus aureus or Streptococcus agalactiae. / Kaartinen, Liisa; Mattila, Tiina; Frost, Alan; Sandholm, Markus.

In: Research in Veterinary Science, Vol. 48, No. 3, 1990, p. 306-309.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Sequestration of N-acetyl-β-D-glucosaminidase in somatic cells during experimental bovine mastitis induced by endotoxin, Staphylococcus aureus or Streptococcus agalactiae

AU - Kaartinen, Liisa

AU - Mattila, Tiina

AU - Frost, Alan

AU - Sandholm, Markus

PY - 1990

Y1 - 1990

N2 - Experimental mastitis was induced in cows by intramammary infusion of endotoxin, Staphylococcus aureus or Streptococcus agalactiae. The inflammatory response was monitored by somatic cell counting and N-acetyl-β-D-glucosaminidase (NAGase). NAGase activity was analysed in fresh milk samples in parallel with samples treated by a cycle of freezing and thawing combined with detergent treatment to release the cell-bound NAGase. Before the udder reacted by inflammation, the total NAGase activity consisted of free extracellular activity. Later on when the inflammation was established, much of the milk NAGase remained sequestered intracellularly. S agalactiae was linked with a high degree of cellular NAGase sequestration indicating a blockage of the lysosomal release function from the phagocytes. S aureus delayed the inflammatory response.

AB - Experimental mastitis was induced in cows by intramammary infusion of endotoxin, Staphylococcus aureus or Streptococcus agalactiae. The inflammatory response was monitored by somatic cell counting and N-acetyl-β-D-glucosaminidase (NAGase). NAGase activity was analysed in fresh milk samples in parallel with samples treated by a cycle of freezing and thawing combined with detergent treatment to release the cell-bound NAGase. Before the udder reacted by inflammation, the total NAGase activity consisted of free extracellular activity. Later on when the inflammation was established, much of the milk NAGase remained sequestered intracellularly. S agalactiae was linked with a high degree of cellular NAGase sequestration indicating a blockage of the lysosomal release function from the phagocytes. S aureus delayed the inflammatory response.

U2 - 10.1016/S0034-5288(18)31018-X

DO - 10.1016/S0034-5288(18)31018-X

M3 - Article

VL - 48

SP - 306

EP - 309

JO - Research in Veterinary Science

JF - Research in Veterinary Science

SN - 0034-5288

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