Serum saturated fatty acids containing triacylglycerols are better markers of insulin resistance than total serum triacylglycerol concentrations

Anna Kotronen, Vidya Velagapudi, Laxman Yetukuri, J. Westerbacka, R. Bergholm, Kim Ekroos, J. Makkonen, M.-R. Taskinen, Matej Orešič (Corresponding Author), H. Yki-Järvinen

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Aims/hypothesis: The weak relationship between insulin resistance and total serum triacylglycerols (TGs) could be in part due to heterogeneity of TG molecules and their distribution within different lipoproteins. We determined concentrations of individual TGs and the fatty acid composition of serum and major lipoprotein particles and analysed how changes in different TGs and fatty acid composition are related to features of insulin resistance and abdominal obesity.

Methods: We performed lipidomic analyses of all major lipoprotein fractions using two analytical platforms in 16 individuals, who exhibited a broad range of insulin sensitivity.

Results: We identified 45 different TGs in serum. Serum TGs containing saturated and monounsaturated fatty acids were positively, while TGs containing essential linoleic acid (18:2 n−6) were negatively correlated with HOMA-IR. Specific serum TGs that correlated positively with HOMA-IR were also significantly positively related to HOMA-IR when measured in very-low-density lipoproteins (VLDLs), intermediate-density lipoproteins (IDLs) and LDL, but not in HDL subfraction 2 (HDL2) or 3 (HDL3). Analyses of proportions of esterified fatty acids within lipoproteins revealed that palmitic acid (16:0) was positively related to HOMA-IR when measured in VLDL, IDL and LDL, but not in HDL2 or HDL3. Monounsaturated palmitoleic (16:1 n−7) and oleic (18:1 n−9) acids were positively related to HOMA-IR when measured in HDL2 and HDL3, but not in VLDL, IDL or LDL. Linoleic acid was negatively related to HOMA-IR in all lipoproteins.

Conclusions/interpretation: Serum concentrations of specific TGs, such as TG(16:0/16:0/18:1) or TG(16:0/18:1/18:0), may be more precise markers of insulin resistance than total serum TG concentrations.
Original languageEnglish
Pages (from-to)684-690
JournalDiabetologia
Volume52
Issue number4
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

Fingerprint

Insulin Resistance
Triglycerides
Fatty Acids
Serum
Lipoproteins
HDL2 Lipoprotein
IDL Lipoproteins
VLDL Lipoproteins
Linoleic Acid
HDL3 Lipoprotein
Monounsaturated Fatty Acids
Palmitic Acid
Abdominal Obesity
Acids

Keywords

  • Insulin resistance
  • Lipidomics
  • Lipoproteins
  • Mass spectrometry
  • Monounsaturated fatty acids
  • Saturated fatty acids
  • Triacylglycerols

Cite this

Kotronen, A., Velagapudi, V., Yetukuri, L., Westerbacka, J., Bergholm, R., Ekroos, K., ... Yki-Järvinen, H. (2009). Serum saturated fatty acids containing triacylglycerols are better markers of insulin resistance than total serum triacylglycerol concentrations. Diabetologia, 52(4), 684-690. https://doi.org/10.1007/s00125-009-1282-2
Kotronen, Anna ; Velagapudi, Vidya ; Yetukuri, Laxman ; Westerbacka, J. ; Bergholm, R. ; Ekroos, Kim ; Makkonen, J. ; Taskinen, M.-R. ; Orešič, Matej ; Yki-Järvinen, H. / Serum saturated fatty acids containing triacylglycerols are better markers of insulin resistance than total serum triacylglycerol concentrations. In: Diabetologia. 2009 ; Vol. 52, No. 4. pp. 684-690.
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abstract = "Aims/hypothesis: The weak relationship between insulin resistance and total serum triacylglycerols (TGs) could be in part due to heterogeneity of TG molecules and their distribution within different lipoproteins. We determined concentrations of individual TGs and the fatty acid composition of serum and major lipoprotein particles and analysed how changes in different TGs and fatty acid composition are related to features of insulin resistance and abdominal obesity.Methods: We performed lipidomic analyses of all major lipoprotein fractions using two analytical platforms in 16 individuals, who exhibited a broad range of insulin sensitivity.Results: We identified 45 different TGs in serum. Serum TGs containing saturated and monounsaturated fatty acids were positively, while TGs containing essential linoleic acid (18:2 n−6) were negatively correlated with HOMA-IR. Specific serum TGs that correlated positively with HOMA-IR were also significantly positively related to HOMA-IR when measured in very-low-density lipoproteins (VLDLs), intermediate-density lipoproteins (IDLs) and LDL, but not in HDL subfraction 2 (HDL2) or 3 (HDL3). Analyses of proportions of esterified fatty acids within lipoproteins revealed that palmitic acid (16:0) was positively related to HOMA-IR when measured in VLDL, IDL and LDL, but not in HDL2 or HDL3. Monounsaturated palmitoleic (16:1 n−7) and oleic (18:1 n−9) acids were positively related to HOMA-IR when measured in HDL2 and HDL3, but not in VLDL, IDL or LDL. Linoleic acid was negatively related to HOMA-IR in all lipoproteins.Conclusions/interpretation: Serum concentrations of specific TGs, such as TG(16:0/16:0/18:1) or TG(16:0/18:1/18:0), may be more precise markers of insulin resistance than total serum TG concentrations.",
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author = "Anna Kotronen and Vidya Velagapudi and Laxman Yetukuri and J. Westerbacka and R. Bergholm and Kim Ekroos and J. Makkonen and M.-R. Taskinen and Matej Orešič and H. Yki-J{\"a}rvinen",
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Kotronen, A, Velagapudi, V, Yetukuri, L, Westerbacka, J, Bergholm, R, Ekroos, K, Makkonen, J, Taskinen, M-R, Orešič, M & Yki-Järvinen, H 2009, 'Serum saturated fatty acids containing triacylglycerols are better markers of insulin resistance than total serum triacylglycerol concentrations', Diabetologia, vol. 52, no. 4, pp. 684-690. https://doi.org/10.1007/s00125-009-1282-2

Serum saturated fatty acids containing triacylglycerols are better markers of insulin resistance than total serum triacylglycerol concentrations. / Kotronen, Anna; Velagapudi, Vidya; Yetukuri, Laxman; Westerbacka, J.; Bergholm, R.; Ekroos, Kim; Makkonen, J.; Taskinen, M.-R.; Orešič, Matej (Corresponding Author); Yki-Järvinen, H.

In: Diabetologia, Vol. 52, No. 4, 2009, p. 684-690.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Serum saturated fatty acids containing triacylglycerols are better markers of insulin resistance than total serum triacylglycerol concentrations

AU - Kotronen, Anna

AU - Velagapudi, Vidya

AU - Yetukuri, Laxman

AU - Westerbacka, J.

AU - Bergholm, R.

AU - Ekroos, Kim

AU - Makkonen, J.

AU - Taskinen, M.-R.

AU - Orešič, Matej

AU - Yki-Järvinen, H.

PY - 2009

Y1 - 2009

N2 - Aims/hypothesis: The weak relationship between insulin resistance and total serum triacylglycerols (TGs) could be in part due to heterogeneity of TG molecules and their distribution within different lipoproteins. We determined concentrations of individual TGs and the fatty acid composition of serum and major lipoprotein particles and analysed how changes in different TGs and fatty acid composition are related to features of insulin resistance and abdominal obesity.Methods: We performed lipidomic analyses of all major lipoprotein fractions using two analytical platforms in 16 individuals, who exhibited a broad range of insulin sensitivity.Results: We identified 45 different TGs in serum. Serum TGs containing saturated and monounsaturated fatty acids were positively, while TGs containing essential linoleic acid (18:2 n−6) were negatively correlated with HOMA-IR. Specific serum TGs that correlated positively with HOMA-IR were also significantly positively related to HOMA-IR when measured in very-low-density lipoproteins (VLDLs), intermediate-density lipoproteins (IDLs) and LDL, but not in HDL subfraction 2 (HDL2) or 3 (HDL3). Analyses of proportions of esterified fatty acids within lipoproteins revealed that palmitic acid (16:0) was positively related to HOMA-IR when measured in VLDL, IDL and LDL, but not in HDL2 or HDL3. Monounsaturated palmitoleic (16:1 n−7) and oleic (18:1 n−9) acids were positively related to HOMA-IR when measured in HDL2 and HDL3, but not in VLDL, IDL or LDL. Linoleic acid was negatively related to HOMA-IR in all lipoproteins.Conclusions/interpretation: Serum concentrations of specific TGs, such as TG(16:0/16:0/18:1) or TG(16:0/18:1/18:0), may be more precise markers of insulin resistance than total serum TG concentrations.

AB - Aims/hypothesis: The weak relationship between insulin resistance and total serum triacylglycerols (TGs) could be in part due to heterogeneity of TG molecules and their distribution within different lipoproteins. We determined concentrations of individual TGs and the fatty acid composition of serum and major lipoprotein particles and analysed how changes in different TGs and fatty acid composition are related to features of insulin resistance and abdominal obesity.Methods: We performed lipidomic analyses of all major lipoprotein fractions using two analytical platforms in 16 individuals, who exhibited a broad range of insulin sensitivity.Results: We identified 45 different TGs in serum. Serum TGs containing saturated and monounsaturated fatty acids were positively, while TGs containing essential linoleic acid (18:2 n−6) were negatively correlated with HOMA-IR. Specific serum TGs that correlated positively with HOMA-IR were also significantly positively related to HOMA-IR when measured in very-low-density lipoproteins (VLDLs), intermediate-density lipoproteins (IDLs) and LDL, but not in HDL subfraction 2 (HDL2) or 3 (HDL3). Analyses of proportions of esterified fatty acids within lipoproteins revealed that palmitic acid (16:0) was positively related to HOMA-IR when measured in VLDL, IDL and LDL, but not in HDL2 or HDL3. Monounsaturated palmitoleic (16:1 n−7) and oleic (18:1 n−9) acids were positively related to HOMA-IR when measured in HDL2 and HDL3, but not in VLDL, IDL or LDL. Linoleic acid was negatively related to HOMA-IR in all lipoproteins.Conclusions/interpretation: Serum concentrations of specific TGs, such as TG(16:0/16:0/18:1) or TG(16:0/18:1/18:0), may be more precise markers of insulin resistance than total serum TG concentrations.

KW - Insulin resistance

KW - Lipidomics

KW - Lipoproteins

KW - Mass spectrometry

KW - Monounsaturated fatty acids

KW - Saturated fatty acids

KW - Triacylglycerols

U2 - 10.1007/s00125-009-1282-2

DO - 10.1007/s00125-009-1282-2

M3 - Article

VL - 52

SP - 684

EP - 690

JO - Diabetologia

JF - Diabetologia

SN - 0012-186X

IS - 4

ER -