Shugoshin 1 plays a central role in kinetochore assembly and is required for kinetochore targeting of Plk1

Jeroen Pouwels, Anu Kukkonen, W. Lan, J.R. Daum, G.J. Gorbsky, T. Stukenberg, Marko Kallio (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

36 Citations (Scopus)

Abstract

Physical connection between the sister chromatids is mediated by the cohesin protein complex. During prophase, cohesin is removed from the chromosome arms while the centromeres remain united. Shugoshin1 (Sgo1) is required for maintenance of centromeric cohesion from prophase to the metaphase-anaphase transition. Furthermore, Sgo1 has been proposed to regulate kinetochore microtubule stability and sense interkinetochore tension, two tasks which are tightly coupled with the function of the Chromosomal Passenger Complex (CPC) and Polo-like kinase 1 (Plk1). Here we show that depletion or chemical inhibition of Aurora B kinase (AurB), the catalytic subunit of the CPC, disrupts accumulation of Sgo1 on the kinetochores in HeLa cells and causes Sgo1 to localize on the chromosome arms. RNAi assays show that depletion of Sgo1 did not affect AurB localization but diminished Plk1 kinetochore binding. Furthermore, we demonstrate that vertebrate Sgo1 is phosphorylated by both AurB and Plk1 in vitro. The data presented here includes an extensive analysis of kinetochore targeting interdependencies of mitotic proteins that propose a novel branch in kinetochore assembly where Sgo1 and Plk1 have central roles. Furthermore our studies implicate Sgo1 in the tension sensing mechanism of the spindle checkpoint by regulating Plk1 kinetochore affinity.
Original languageEnglish
Pages (from-to)1579-1585
JournalCell Cycle
Volume6
Issue number13
DOIs
Publication statusPublished - 2007
MoE publication typeA1 Journal article-refereed

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Kinetochores
Aurora Kinase B
Prophase
Chromosomes
Anaphase
Chromatids
Centromere
Metaphase
RNA Interference
HeLa Cells
Microtubules
Vertebrates
polo-like kinase 1
Catalytic Domain
Maintenance
Proteins

Cite this

Pouwels, J., Kukkonen, A., Lan, W., Daum, J. R., Gorbsky, G. J., Stukenberg, T., & Kallio, M. (2007). Shugoshin 1 plays a central role in kinetochore assembly and is required for kinetochore targeting of Plk1. Cell Cycle, 6(13), 1579-1585. https://doi.org/10.4161/cc.6.13.4442
Pouwels, Jeroen ; Kukkonen, Anu ; Lan, W. ; Daum, J.R. ; Gorbsky, G.J. ; Stukenberg, T. ; Kallio, Marko. / Shugoshin 1 plays a central role in kinetochore assembly and is required for kinetochore targeting of Plk1. In: Cell Cycle. 2007 ; Vol. 6, No. 13. pp. 1579-1585.
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abstract = "Physical connection between the sister chromatids is mediated by the cohesin protein complex. During prophase, cohesin is removed from the chromosome arms while the centromeres remain united. Shugoshin1 (Sgo1) is required for maintenance of centromeric cohesion from prophase to the metaphase-anaphase transition. Furthermore, Sgo1 has been proposed to regulate kinetochore microtubule stability and sense interkinetochore tension, two tasks which are tightly coupled with the function of the Chromosomal Passenger Complex (CPC) and Polo-like kinase 1 (Plk1). Here we show that depletion or chemical inhibition of Aurora B kinase (AurB), the catalytic subunit of the CPC, disrupts accumulation of Sgo1 on the kinetochores in HeLa cells and causes Sgo1 to localize on the chromosome arms. RNAi assays show that depletion of Sgo1 did not affect AurB localization but diminished Plk1 kinetochore binding. Furthermore, we demonstrate that vertebrate Sgo1 is phosphorylated by both AurB and Plk1 in vitro. The data presented here includes an extensive analysis of kinetochore targeting interdependencies of mitotic proteins that propose a novel branch in kinetochore assembly where Sgo1 and Plk1 have central roles. Furthermore our studies implicate Sgo1 in the tension sensing mechanism of the spindle checkpoint by regulating Plk1 kinetochore affinity.",
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Pouwels, J, Kukkonen, A, Lan, W, Daum, JR, Gorbsky, GJ, Stukenberg, T & Kallio, M 2007, 'Shugoshin 1 plays a central role in kinetochore assembly and is required for kinetochore targeting of Plk1', Cell Cycle, vol. 6, no. 13, pp. 1579-1585. https://doi.org/10.4161/cc.6.13.4442

Shugoshin 1 plays a central role in kinetochore assembly and is required for kinetochore targeting of Plk1. / Pouwels, Jeroen; Kukkonen, Anu; Lan, W.; Daum, J.R.; Gorbsky, G.J.; Stukenberg, T.; Kallio, Marko (Corresponding Author).

In: Cell Cycle, Vol. 6, No. 13, 2007, p. 1579-1585.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Shugoshin 1 plays a central role in kinetochore assembly and is required for kinetochore targeting of Plk1

AU - Pouwels, Jeroen

AU - Kukkonen, Anu

AU - Lan, W.

AU - Daum, J.R.

AU - Gorbsky, G.J.

AU - Stukenberg, T.

AU - Kallio, Marko

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AB - Physical connection between the sister chromatids is mediated by the cohesin protein complex. During prophase, cohesin is removed from the chromosome arms while the centromeres remain united. Shugoshin1 (Sgo1) is required for maintenance of centromeric cohesion from prophase to the metaphase-anaphase transition. Furthermore, Sgo1 has been proposed to regulate kinetochore microtubule stability and sense interkinetochore tension, two tasks which are tightly coupled with the function of the Chromosomal Passenger Complex (CPC) and Polo-like kinase 1 (Plk1). Here we show that depletion or chemical inhibition of Aurora B kinase (AurB), the catalytic subunit of the CPC, disrupts accumulation of Sgo1 on the kinetochores in HeLa cells and causes Sgo1 to localize on the chromosome arms. RNAi assays show that depletion of Sgo1 did not affect AurB localization but diminished Plk1 kinetochore binding. Furthermore, we demonstrate that vertebrate Sgo1 is phosphorylated by both AurB and Plk1 in vitro. The data presented here includes an extensive analysis of kinetochore targeting interdependencies of mitotic proteins that propose a novel branch in kinetochore assembly where Sgo1 and Plk1 have central roles. Furthermore our studies implicate Sgo1 in the tension sensing mechanism of the spindle checkpoint by regulating Plk1 kinetochore affinity.

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DO - 10.4161/cc.6.13.4442

M3 - Article

VL - 6

SP - 1579

EP - 1585

JO - Cell Cycle

JF - Cell Cycle

SN - 1538-4101

IS - 13

ER -