SigC sigma factor is involved in acclimation to low inorganic carbon at high temperature in Synechocystis sp. PCC 6803

Liisa Gunnelius, Ilona Tuominen, Susanne Rantamäki, Maija Pollari, Virpi Ruotsalainen, Esa Tyystjärvi, Taina Tyystjärvi (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

Inactivation of the sigC gene (sll0184), encoding the group 2 sigma factor SigC, leads to a heat-sensitive phenotype of Synechocystis sp. PCC 6803. Cells of the ΔsigC strain grew poorly at 43 °C at pH 7.5 under ambient CO2 conditions. Addition of inorganic carbon in the form of 3 % CO2 or use of an alkaline growth medium (pH 8.3) restored the growth of the ΔsigC strain at 43 °C. These treatments compensate for the low concentration of inorganic carbon at high temperature. However, addition of organic carbon as glucose, pyruvate, succinate or 2-oxoglutarate did not restore growth of the ΔsigC strain at 43 °C. In the control strain, the amount of the SigC factor diminished after prolonged incubation at 43 °C if the pH of the growth medium was 7.5 or 6.7. Under alkaline conditions, the amount of the SigC factor remained constant at 43 °C and cells of the control strain grew better than at pH 7.5 or pH 6.7. The pH dependence of high-temperature growth was associated with changes in photosynthetic activity, indicating that the SigC factor is involved in adjustment of photosynthesis according to the amount of available inorganic carbon. Our results indicate that acclimation to low inorganic carbon is a part of acclimation to prolonged high temperature and that the SigC factor has a central role in this acclimation.
Original languageEnglish
Pages (from-to)220-229
JournalMicrobiology
Volume156
Issue number1
DOIs
Publication statusPublished - 2010
MoE publication typeA1 Journal article-refereed

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Synechocystis
Sigma Factor
Acclimatization
Carbon
Temperature
Growth
Photosynthesis
Succinic Acid
Gene Silencing
Pyruvic Acid
Hot Temperature
Phenotype
Glucose

Cite this

Gunnelius, L., Tuominen, I., Rantamäki, S., Pollari, M., Ruotsalainen, V., Tyystjärvi, E., & Tyystjärvi, T. (2010). SigC sigma factor is involved in acclimation to low inorganic carbon at high temperature in Synechocystis sp. PCC 6803. Microbiology, 156(1), 220-229. https://doi.org/10.1099/mic.0.032565-0
Gunnelius, Liisa ; Tuominen, Ilona ; Rantamäki, Susanne ; Pollari, Maija ; Ruotsalainen, Virpi ; Tyystjärvi, Esa ; Tyystjärvi, Taina. / SigC sigma factor is involved in acclimation to low inorganic carbon at high temperature in Synechocystis sp. PCC 6803. In: Microbiology. 2010 ; Vol. 156, No. 1. pp. 220-229.
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title = "SigC sigma factor is involved in acclimation to low inorganic carbon at high temperature in Synechocystis sp. PCC 6803",
abstract = "Inactivation of the sigC gene (sll0184), encoding the group 2 sigma factor SigC, leads to a heat-sensitive phenotype of Synechocystis sp. PCC 6803. Cells of the ΔsigC strain grew poorly at 43 °C at pH 7.5 under ambient CO2 conditions. Addition of inorganic carbon in the form of 3 {\%} CO2 or use of an alkaline growth medium (pH 8.3) restored the growth of the ΔsigC strain at 43 °C. These treatments compensate for the low concentration of inorganic carbon at high temperature. However, addition of organic carbon as glucose, pyruvate, succinate or 2-oxoglutarate did not restore growth of the ΔsigC strain at 43 °C. In the control strain, the amount of the SigC factor diminished after prolonged incubation at 43 °C if the pH of the growth medium was 7.5 or 6.7. Under alkaline conditions, the amount of the SigC factor remained constant at 43 °C and cells of the control strain grew better than at pH 7.5 or pH 6.7. The pH dependence of high-temperature growth was associated with changes in photosynthetic activity, indicating that the SigC factor is involved in adjustment of photosynthesis according to the amount of available inorganic carbon. Our results indicate that acclimation to low inorganic carbon is a part of acclimation to prolonged high temperature and that the SigC factor has a central role in this acclimation.",
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Gunnelius, L, Tuominen, I, Rantamäki, S, Pollari, M, Ruotsalainen, V, Tyystjärvi, E & Tyystjärvi, T 2010, 'SigC sigma factor is involved in acclimation to low inorganic carbon at high temperature in Synechocystis sp. PCC 6803', Microbiology, vol. 156, no. 1, pp. 220-229. https://doi.org/10.1099/mic.0.032565-0

SigC sigma factor is involved in acclimation to low inorganic carbon at high temperature in Synechocystis sp. PCC 6803. / Gunnelius, Liisa; Tuominen, Ilona; Rantamäki, Susanne; Pollari, Maija; Ruotsalainen, Virpi; Tyystjärvi, Esa; Tyystjärvi, Taina (Corresponding Author).

In: Microbiology, Vol. 156, No. 1, 2010, p. 220-229.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - SigC sigma factor is involved in acclimation to low inorganic carbon at high temperature in Synechocystis sp. PCC 6803

AU - Gunnelius, Liisa

AU - Tuominen, Ilona

AU - Rantamäki, Susanne

AU - Pollari, Maija

AU - Ruotsalainen, Virpi

AU - Tyystjärvi, Esa

AU - Tyystjärvi, Taina

PY - 2010

Y1 - 2010

N2 - Inactivation of the sigC gene (sll0184), encoding the group 2 sigma factor SigC, leads to a heat-sensitive phenotype of Synechocystis sp. PCC 6803. Cells of the ΔsigC strain grew poorly at 43 °C at pH 7.5 under ambient CO2 conditions. Addition of inorganic carbon in the form of 3 % CO2 or use of an alkaline growth medium (pH 8.3) restored the growth of the ΔsigC strain at 43 °C. These treatments compensate for the low concentration of inorganic carbon at high temperature. However, addition of organic carbon as glucose, pyruvate, succinate or 2-oxoglutarate did not restore growth of the ΔsigC strain at 43 °C. In the control strain, the amount of the SigC factor diminished after prolonged incubation at 43 °C if the pH of the growth medium was 7.5 or 6.7. Under alkaline conditions, the amount of the SigC factor remained constant at 43 °C and cells of the control strain grew better than at pH 7.5 or pH 6.7. The pH dependence of high-temperature growth was associated with changes in photosynthetic activity, indicating that the SigC factor is involved in adjustment of photosynthesis according to the amount of available inorganic carbon. Our results indicate that acclimation to low inorganic carbon is a part of acclimation to prolonged high temperature and that the SigC factor has a central role in this acclimation.

AB - Inactivation of the sigC gene (sll0184), encoding the group 2 sigma factor SigC, leads to a heat-sensitive phenotype of Synechocystis sp. PCC 6803. Cells of the ΔsigC strain grew poorly at 43 °C at pH 7.5 under ambient CO2 conditions. Addition of inorganic carbon in the form of 3 % CO2 or use of an alkaline growth medium (pH 8.3) restored the growth of the ΔsigC strain at 43 °C. These treatments compensate for the low concentration of inorganic carbon at high temperature. However, addition of organic carbon as glucose, pyruvate, succinate or 2-oxoglutarate did not restore growth of the ΔsigC strain at 43 °C. In the control strain, the amount of the SigC factor diminished after prolonged incubation at 43 °C if the pH of the growth medium was 7.5 or 6.7. Under alkaline conditions, the amount of the SigC factor remained constant at 43 °C and cells of the control strain grew better than at pH 7.5 or pH 6.7. The pH dependence of high-temperature growth was associated with changes in photosynthetic activity, indicating that the SigC factor is involved in adjustment of photosynthesis according to the amount of available inorganic carbon. Our results indicate that acclimation to low inorganic carbon is a part of acclimation to prolonged high temperature and that the SigC factor has a central role in this acclimation.

U2 - 10.1099/mic.0.032565-0

DO - 10.1099/mic.0.032565-0

M3 - Article

VL - 156

SP - 220

EP - 229

JO - Microbiology

JF - Microbiology

SN - 1350-0872

IS - 1

ER -

Gunnelius L, Tuominen I, Rantamäki S, Pollari M, Ruotsalainen V, Tyystjärvi E et al. SigC sigma factor is involved in acclimation to low inorganic carbon at high temperature in Synechocystis sp. PCC 6803. Microbiology. 2010;156(1):220-229. https://doi.org/10.1099/mic.0.032565-0