Silicon-glass instrumented solid-phase extraction-zone electrophoresis microchip with thin amorphous silicon film electrodes: Performance in immunoaffinity analysis

Ari Hokkanen (Corresponding Author), Heli Sirén, Lotta Amundsen, Kai Kolari, Sami Franssila, Santeri Tuomikoski, Ingmar Stuns, Stella Rovio, Tarja Nevanen, Kristiina Takkinen, Hans Söderlund

Research output: Contribution to journalArticleScientificpeer-review

6 Citations (Scopus)

Abstract

Silicon–glass microchips were designed and fabricated for on-chip solid phase extraction (SPE) and zone electrophoresis studies. The solvent channels for extraction and the separation channels for analyses were fabricated sequentially on the silicon device. Electrical contacts were integrated in a fused silica glass lid. Amorphous silicon thin film electrodes were fabricated for high voltage and conductivity detection. A chip installation rack with electrical and fluidic contacts was constructed to facilitate the experiments. Simulation was used to elucidate both the liquid flow and the electric field distribution. The operational performance of the microchips was demonstrated by using a fluorescein isothiocyanate (FITC)-labelled testosterone derivative as the model analyte and fluorescein as both the negative control and the calibration compounds. In SPE an immunosorbent, based on recombinant anti-testosterone Fab-fragments, was immobilized to activated Sepharose gel. Simultaneous monitoring of the movement of FITC-testosterone from SPE cavity through the channel to the detection point was performed with a laser-induced fluorescence detector. The observed limit of detection for FITC-testosterone was 2 μM.
Original languageEnglish
Pages (from-to)611-619
Number of pages9
JournalMicrosystem Technologies
Volume15
Issue number4
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

Fingerprint

Silicon
electrophoresis
silicon films
Electrophoresis
Fluorescein
Amorphous silicon
amorphous silicon
Testosterone
solid phases
Glass
Electrodes
electrodes
glass
silicon
Fused silica
chips
racks
Immunosorbents
Immunoglobulin Fab Fragments
liquid flow

Cite this

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title = "Silicon-glass instrumented solid-phase extraction-zone electrophoresis microchip with thin amorphous silicon film electrodes: Performance in immunoaffinity analysis",
abstract = "Silicon–glass microchips were designed and fabricated for on-chip solid phase extraction (SPE) and zone electrophoresis studies. The solvent channels for extraction and the separation channels for analyses were fabricated sequentially on the silicon device. Electrical contacts were integrated in a fused silica glass lid. Amorphous silicon thin film electrodes were fabricated for high voltage and conductivity detection. A chip installation rack with electrical and fluidic contacts was constructed to facilitate the experiments. Simulation was used to elucidate both the liquid flow and the electric field distribution. The operational performance of the microchips was demonstrated by using a fluorescein isothiocyanate (FITC)-labelled testosterone derivative as the model analyte and fluorescein as both the negative control and the calibration compounds. In SPE an immunosorbent, based on recombinant anti-testosterone Fab-fragments, was immobilized to activated Sepharose gel. Simultaneous monitoring of the movement of FITC-testosterone from SPE cavity through the channel to the detection point was performed with a laser-induced fluorescence detector. The observed limit of detection for FITC-testosterone was 2 μM.",
author = "Ari Hokkanen and Heli Sir{\'e}n and Lotta Amundsen and Kai Kolari and Sami Franssila and Santeri Tuomikoski and Ingmar Stuns and Stella Rovio and Tarja Nevanen and Kristiina Takkinen and Hans S{\"o}derlund",
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Silicon-glass instrumented solid-phase extraction-zone electrophoresis microchip with thin amorphous silicon film electrodes : Performance in immunoaffinity analysis. / Hokkanen, Ari (Corresponding Author); Sirén, Heli; Amundsen, Lotta; Kolari, Kai; Franssila, Sami; Tuomikoski, Santeri; Stuns, Ingmar; Rovio, Stella; Nevanen, Tarja; Takkinen, Kristiina; Söderlund, Hans.

In: Microsystem Technologies, Vol. 15, No. 4, 2009, p. 611-619.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Silicon-glass instrumented solid-phase extraction-zone electrophoresis microchip with thin amorphous silicon film electrodes

T2 - Performance in immunoaffinity analysis

AU - Hokkanen, Ari

AU - Sirén, Heli

AU - Amundsen, Lotta

AU - Kolari, Kai

AU - Franssila, Sami

AU - Tuomikoski, Santeri

AU - Stuns, Ingmar

AU - Rovio, Stella

AU - Nevanen, Tarja

AU - Takkinen, Kristiina

AU - Söderlund, Hans

PY - 2009

Y1 - 2009

N2 - Silicon–glass microchips were designed and fabricated for on-chip solid phase extraction (SPE) and zone electrophoresis studies. The solvent channels for extraction and the separation channels for analyses were fabricated sequentially on the silicon device. Electrical contacts were integrated in a fused silica glass lid. Amorphous silicon thin film electrodes were fabricated for high voltage and conductivity detection. A chip installation rack with electrical and fluidic contacts was constructed to facilitate the experiments. Simulation was used to elucidate both the liquid flow and the electric field distribution. The operational performance of the microchips was demonstrated by using a fluorescein isothiocyanate (FITC)-labelled testosterone derivative as the model analyte and fluorescein as both the negative control and the calibration compounds. In SPE an immunosorbent, based on recombinant anti-testosterone Fab-fragments, was immobilized to activated Sepharose gel. Simultaneous monitoring of the movement of FITC-testosterone from SPE cavity through the channel to the detection point was performed with a laser-induced fluorescence detector. The observed limit of detection for FITC-testosterone was 2 μM.

AB - Silicon–glass microchips were designed and fabricated for on-chip solid phase extraction (SPE) and zone electrophoresis studies. The solvent channels for extraction and the separation channels for analyses were fabricated sequentially on the silicon device. Electrical contacts were integrated in a fused silica glass lid. Amorphous silicon thin film electrodes were fabricated for high voltage and conductivity detection. A chip installation rack with electrical and fluidic contacts was constructed to facilitate the experiments. Simulation was used to elucidate both the liquid flow and the electric field distribution. The operational performance of the microchips was demonstrated by using a fluorescein isothiocyanate (FITC)-labelled testosterone derivative as the model analyte and fluorescein as both the negative control and the calibration compounds. In SPE an immunosorbent, based on recombinant anti-testosterone Fab-fragments, was immobilized to activated Sepharose gel. Simultaneous monitoring of the movement of FITC-testosterone from SPE cavity through the channel to the detection point was performed with a laser-induced fluorescence detector. The observed limit of detection for FITC-testosterone was 2 μM.

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M3 - Article

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SP - 611

EP - 619

JO - Microsystem Technologies

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SN - 0946-7076

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