Simultaneous determination of scopolamine, hyoscyamine and littorine in plants and different hairy root clones of Hyoscyamus muticus by micellar electrokinetic chromatography

Lidia Mateus, Samir Cherkaoui, Philippe Christen (Corresponding Author), Kirsi-Marja Oksman-Caldentey

Research output: Contribution to journalArticleScientificpeer-review

45 Citations (Scopus)

Abstract

Hyoscyamus muticus hairy root clones were established following infection with Agrobacterium rhizogenes strains A4, LBA-9402 and 15834 and with A. tumefaciens strain C58C1pRTGus104. The accumulation of tropane alkaloids hyoscyamine, littorine and scopolamine was evaluated by micellar electrokinetic capillary electrophoresis. Littorine was reported for the first time in these clones as well as in the roots of the intact plant and confirmed by collision induced dissociation-mass spectrometry. Tropane alkaloid content in hairy roots was compared with leaves and roots of normal plants at two vegetative stages. Significant differences appeared between the alkaloid contents of the different clones. In particular, all the hairy root clones and the roots of the intact plant produced 1.5–3 and 4.5–9 times more littorine than scopolamine, respectively. The only exception was clone KB7, carrying the h6h gene, which overproduced scopolamine. The aerial parts of H. muticus plants did not contain any littorine, thus indicating different transportation or translocation mechanisms of the various tropane alkaloids.
Original languageEnglish
Pages (from-to)517 - 523
Number of pages7
JournalPhytochemistry
Volume54
Issue number5
DOIs
Publication statusPublished - 2000
MoE publication typeA1 Journal article-refereed

Fingerprint

Hyoscyamus
Hyoscyamine
Hyoscyamus muticus
scopolamine
Scopolamine Hydrobromide
atropine
Tropanes
Chromatography
Alkaloids
chromatography
Clone Cells
Plant Roots
clones
tropane alkaloids
Capillary electrophoresis
Agrobacterium
Mass spectrometry
Capillary Electrophoresis
Rhizobium rhizogenes
Genes

Keywords

  • Hyoscyamus muticus
  • Solanaceae
  • Egyptian henbane
  • Hairy root clones
  • Tropane alkaloids
  • Scopolamine
  • Hyoscyamine
  • Littorine

Cite this

@article{f8131eab9bd147bab00214b24f1e2901,
title = "Simultaneous determination of scopolamine, hyoscyamine and littorine in plants and different hairy root clones of Hyoscyamus muticus by micellar electrokinetic chromatography",
abstract = "Hyoscyamus muticus hairy root clones were established following infection with Agrobacterium rhizogenes strains A4, LBA-9402 and 15834 and with A. tumefaciens strain C58C1pRTGus104. The accumulation of tropane alkaloids hyoscyamine, littorine and scopolamine was evaluated by micellar electrokinetic capillary electrophoresis. Littorine was reported for the first time in these clones as well as in the roots of the intact plant and confirmed by collision induced dissociation-mass spectrometry. Tropane alkaloid content in hairy roots was compared with leaves and roots of normal plants at two vegetative stages. Significant differences appeared between the alkaloid contents of the different clones. In particular, all the hairy root clones and the roots of the intact plant produced 1.5–3 and 4.5–9 times more littorine than scopolamine, respectively. The only exception was clone KB7, carrying the h6h gene, which overproduced scopolamine. The aerial parts of H. muticus plants did not contain any littorine, thus indicating different transportation or translocation mechanisms of the various tropane alkaloids.",
keywords = "Hyoscyamus muticus, Solanaceae, Egyptian henbane, Hairy root clones, Tropane alkaloids, Scopolamine, Hyoscyamine, Littorine",
author = "Lidia Mateus and Samir Cherkaoui and Philippe Christen and Kirsi-Marja Oksman-Caldentey",
year = "2000",
doi = "10.1016/S0031-9422(00)00134-5",
language = "English",
volume = "54",
pages = "517 -- 523",
journal = "Phytochemistry",
issn = "0031-9422",
publisher = "Elsevier",
number = "5",

}

Simultaneous determination of scopolamine, hyoscyamine and littorine in plants and different hairy root clones of Hyoscyamus muticus by micellar electrokinetic chromatography. / Mateus, Lidia; Cherkaoui, Samir; Christen, Philippe (Corresponding Author); Oksman-Caldentey, Kirsi-Marja.

In: Phytochemistry, Vol. 54, No. 5, 2000, p. 517 - 523.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Simultaneous determination of scopolamine, hyoscyamine and littorine in plants and different hairy root clones of Hyoscyamus muticus by micellar electrokinetic chromatography

AU - Mateus, Lidia

AU - Cherkaoui, Samir

AU - Christen, Philippe

AU - Oksman-Caldentey, Kirsi-Marja

PY - 2000

Y1 - 2000

N2 - Hyoscyamus muticus hairy root clones were established following infection with Agrobacterium rhizogenes strains A4, LBA-9402 and 15834 and with A. tumefaciens strain C58C1pRTGus104. The accumulation of tropane alkaloids hyoscyamine, littorine and scopolamine was evaluated by micellar electrokinetic capillary electrophoresis. Littorine was reported for the first time in these clones as well as in the roots of the intact plant and confirmed by collision induced dissociation-mass spectrometry. Tropane alkaloid content in hairy roots was compared with leaves and roots of normal plants at two vegetative stages. Significant differences appeared between the alkaloid contents of the different clones. In particular, all the hairy root clones and the roots of the intact plant produced 1.5–3 and 4.5–9 times more littorine than scopolamine, respectively. The only exception was clone KB7, carrying the h6h gene, which overproduced scopolamine. The aerial parts of H. muticus plants did not contain any littorine, thus indicating different transportation or translocation mechanisms of the various tropane alkaloids.

AB - Hyoscyamus muticus hairy root clones were established following infection with Agrobacterium rhizogenes strains A4, LBA-9402 and 15834 and with A. tumefaciens strain C58C1pRTGus104. The accumulation of tropane alkaloids hyoscyamine, littorine and scopolamine was evaluated by micellar electrokinetic capillary electrophoresis. Littorine was reported for the first time in these clones as well as in the roots of the intact plant and confirmed by collision induced dissociation-mass spectrometry. Tropane alkaloid content in hairy roots was compared with leaves and roots of normal plants at two vegetative stages. Significant differences appeared between the alkaloid contents of the different clones. In particular, all the hairy root clones and the roots of the intact plant produced 1.5–3 and 4.5–9 times more littorine than scopolamine, respectively. The only exception was clone KB7, carrying the h6h gene, which overproduced scopolamine. The aerial parts of H. muticus plants did not contain any littorine, thus indicating different transportation or translocation mechanisms of the various tropane alkaloids.

KW - Hyoscyamus muticus

KW - Solanaceae

KW - Egyptian henbane

KW - Hairy root clones

KW - Tropane alkaloids

KW - Scopolamine

KW - Hyoscyamine

KW - Littorine

U2 - 10.1016/S0031-9422(00)00134-5

DO - 10.1016/S0031-9422(00)00134-5

M3 - Article

VL - 54

SP - 517

EP - 523

JO - Phytochemistry

JF - Phytochemistry

SN - 0031-9422

IS - 5

ER -