Simultaneous determination of scopolamine, hyoscyamine and littorine in plants and different hairy root clones of Hyoscyamus muticus by micellar electrokinetic chromatography

Lidia Mateus, Samir Cherkaoui, Philippe Christen (Corresponding Author), Kirsi-Marja Oksman-Caldentey

Research output: Contribution to journalArticleScientificpeer-review

47 Citations (Scopus)

Abstract

Hyoscyamus muticus hairy root clones were established following infection with Agrobacterium rhizogenes strains A4, LBA-9402 and 15834 and with A. tumefaciens strain C58C1pRTGus104. The accumulation of tropane alkaloids hyoscyamine, littorine and scopolamine was evaluated by micellar electrokinetic capillary electrophoresis. Littorine was reported for the first time in these clones as well as in the roots of the intact plant and confirmed by collision induced dissociation-mass spectrometry. Tropane alkaloid content in hairy roots was compared with leaves and roots of normal plants at two vegetative stages. Significant differences appeared between the alkaloid contents of the different clones. In particular, all the hairy root clones and the roots of the intact plant produced 1.5–3 and 4.5–9 times more littorine than scopolamine, respectively. The only exception was clone KB7, carrying the h6h gene, which overproduced scopolamine. The aerial parts of H. muticus plants did not contain any littorine, thus indicating different transportation or translocation mechanisms of the various tropane alkaloids.
Original languageEnglish
Pages (from-to)517 - 523
Number of pages7
JournalPhytochemistry
Volume54
Issue number5
DOIs
Publication statusPublished - 2000
MoE publication typeA1 Journal article-refereed

Keywords

  • Hyoscyamus muticus
  • Solanaceae
  • Egyptian henbane
  • Hairy root clones
  • Tropane alkaloids
  • Scopolamine
  • Hyoscyamine
  • Littorine

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