Size-exclusion chromatographic determination of β-glucan with postcolumn reaction detection

Tapani Suortti

Research output: Contribution to journalArticleScientificpeer-review

55 Citations (Scopus)

Abstract

Using the specific interaction of β-glucan with calcofluor dye as a detection method for size-exclusion chromatographic analysis, very simple direct extractions can be used for sample preparation. Either fluorescence or UV detection is employed. The calibration graphs are linear between 200 and 20 mg/l, although much more sensitive analyses are possible. The response remained independent of relative molecular mass in the range studied (1.7·106-1.85·105). Results for barley and malt show good correlation with those obtained by flow- injection analysis using calcofluor.

Original languageEnglish
Pages (from-to)105 - 110
Number of pages6
JournalJournal of Chromatography
Volume632
Issue number1-2
DOIs
Publication statusPublished - 1993
MoE publication typeA1 Journal article-refereed

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Flow Injection Analysis
Glucans
Size exclusion chromatography
Molecular mass
Hordeum
Calibration
Gel Chromatography
Coloring Agents
Fluorescence

Cite this

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title = "Size-exclusion chromatographic determination of β-glucan with postcolumn reaction detection",
abstract = "Using the specific interaction of β-glucan with calcofluor dye as a detection method for size-exclusion chromatographic analysis, very simple direct extractions can be used for sample preparation. Either fluorescence or UV detection is employed. The calibration graphs are linear between 200 and 20 mg/l, although much more sensitive analyses are possible. The response remained independent of relative molecular mass in the range studied (1.7·106-1.85·105). Results for barley and malt show good correlation with those obtained by flow- injection analysis using calcofluor.",
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Size-exclusion chromatographic determination of β-glucan with postcolumn reaction detection. / Suortti, Tapani.

In: Journal of Chromatography, Vol. 632, No. 1-2, 1993, p. 105 - 110.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Size-exclusion chromatographic determination of β-glucan with postcolumn reaction detection

AU - Suortti, Tapani

PY - 1993

Y1 - 1993

N2 - Using the specific interaction of β-glucan with calcofluor dye as a detection method for size-exclusion chromatographic analysis, very simple direct extractions can be used for sample preparation. Either fluorescence or UV detection is employed. The calibration graphs are linear between 200 and 20 mg/l, although much more sensitive analyses are possible. The response remained independent of relative molecular mass in the range studied (1.7·106-1.85·105). Results for barley and malt show good correlation with those obtained by flow- injection analysis using calcofluor.

AB - Using the specific interaction of β-glucan with calcofluor dye as a detection method for size-exclusion chromatographic analysis, very simple direct extractions can be used for sample preparation. Either fluorescence or UV detection is employed. The calibration graphs are linear between 200 and 20 mg/l, although much more sensitive analyses are possible. The response remained independent of relative molecular mass in the range studied (1.7·106-1.85·105). Results for barley and malt show good correlation with those obtained by flow- injection analysis using calcofluor.

U2 - 10.1016/0021-9673(93)80032-4

DO - 10.1016/0021-9673(93)80032-4

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JO - Journal of Chromatography A

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