Size-exclusion chromatography of xylan derivatives - The critical evaluation of macromolecular data

Leena Pitkänen, Vladimir Aseyev, Christiane Laine, Päivi Tuomainen, Maija Tenkanen

    Research output: Contribution to journalArticleScientificpeer-review

    2 Citations (Scopus)

    Abstract

    Hydroxypropyl xylans with varying degrees of substitution were characterized by size-exclusion chromatography. Molar masses of the samples were determined using two approaches: by conventional calibration with molar mass standards and by a multi-detection method that utilizes the combination of static light scattering, viscometry, and differential refractive index detection. The molar mass results obtained by the multi-detection method were accurate, but required the determination of separate refractive index increments for each structurally different sample. The column calibration approach with standard pullulan samples gave biased results due to the differences in hydrodynamic volumes between pullulans and hydroxypropyl xylans with similar molar masses. The degree of hydroxypropylation affected the chain conformation and compactness of the polymer chains. Mark-Houwink parameters and persistence length values suggested that the hydroxypropyl substituents reduced the flexibility of the xylan chain and made the polymer chain more extended.

    Original languageEnglish
    Pages (from-to)4811-4817
    Number of pages7
    JournalAnalytical and Bioanalytical Chemistry
    Volume409
    Issue number20
    DOIs
    Publication statusPublished - 1 Aug 2017
    MoE publication typeA1 Journal article-refereed

    Fingerprint

    Xylans
    Size exclusion chromatography
    Molar mass
    Gel Chromatography
    Refractometry
    Derivatives
    Calibration
    Polymers
    Refractive index
    Hydrodynamics
    Viscosity measurement
    Light scattering
    Conformations
    Light
    Substitution reactions
    pullulan

    Keywords

    • xylans
    • hydroxypropylation
    • size-exclusion chromatography
    • chain stiffness

    Cite this

    Pitkänen, Leena ; Aseyev, Vladimir ; Laine, Christiane ; Tuomainen, Päivi ; Tenkanen, Maija. / Size-exclusion chromatography of xylan derivatives - The critical evaluation of macromolecular data. In: Analytical and Bioanalytical Chemistry. 2017 ; Vol. 409, No. 20. pp. 4811-4817.
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    abstract = "Hydroxypropyl xylans with varying degrees of substitution were characterized by size-exclusion chromatography. Molar masses of the samples were determined using two approaches: by conventional calibration with molar mass standards and by a multi-detection method that utilizes the combination of static light scattering, viscometry, and differential refractive index detection. The molar mass results obtained by the multi-detection method were accurate, but required the determination of separate refractive index increments for each structurally different sample. The column calibration approach with standard pullulan samples gave biased results due to the differences in hydrodynamic volumes between pullulans and hydroxypropyl xylans with similar molar masses. The degree of hydroxypropylation affected the chain conformation and compactness of the polymer chains. Mark-Houwink parameters and persistence length values suggested that the hydroxypropyl substituents reduced the flexibility of the xylan chain and made the polymer chain more extended.",
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    Size-exclusion chromatography of xylan derivatives - The critical evaluation of macromolecular data. / Pitkänen, Leena; Aseyev, Vladimir; Laine, Christiane; Tuomainen, Päivi; Tenkanen, Maija.

    In: Analytical and Bioanalytical Chemistry, Vol. 409, No. 20, 01.08.2017, p. 4811-4817.

    Research output: Contribution to journalArticleScientificpeer-review

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    AU - Pitkänen, Leena

    AU - Aseyev, Vladimir

    AU - Laine, Christiane

    AU - Tuomainen, Päivi

    AU - Tenkanen, Maija

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