Small-angle scattering study of structural changes in the microfibril network of nanocellulose during enzymatic hydrolysis

P.A. Penttilä (Corresponding Author), A. Várnai, M. Fernández, I. Kontro, V. Liljeström, P. Lindner, Matti Siika-aho, L. Viikari, R. Serimaa

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

The hydrolysis of nanofibrillated cellulose (NFC), consisting of individual cellulose fibrils, was followed using small-angle scattering techniques in order to reveal changes in the substrate structure caused by cellulose degrading enzymes. In particular, the nanoscale structure of the network of cellulose fibrils was characterized with the combination of small-angle neutron scattering and small-angle x-ray scattering. In the nanocellulose with higher xylan content, the interfibrillar distance was shown to remain unchanged during enzymatic degradation, whereas the distance increased in the nanocellulose with lower xylan content. The limiting effect of xylan on the hydrolysis and a faster hydrolysis of the more thoroughly fibrillated segments of the NFC network could be observed. Despite the extensive fibrillation of the raw material, however, the hydrolysis was eventually limited by the aggregated and heterogeneous structure of the substrate.
Original languageEnglish
Pages (from-to)1031-1040
Number of pages10
JournalCellulose
Volume20
Issue number3
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

Fingerprint

Enzymatic hydrolysis
Cellulose
Xylans
Scattering
Hydrolysis
Substrates
Neutron scattering
Raw materials
Enzymes
Degradation
X rays

Keywords

  • bioethanol
  • cellulose
  • enzymatic hydrolysis
  • hemicellulase
  • SANS
  • SAXS

Cite this

Penttilä, P. A., Várnai, A., Fernández, M., Kontro, I., Liljeström, V., Lindner, P., ... Serimaa, R. (2013). Small-angle scattering study of structural changes in the microfibril network of nanocellulose during enzymatic hydrolysis. Cellulose, 20(3), 1031-1040. https://doi.org/10.1007/s10570-013-9899-1
Penttilä, P.A. ; Várnai, A. ; Fernández, M. ; Kontro, I. ; Liljeström, V. ; Lindner, P. ; Siika-aho, Matti ; Viikari, L. ; Serimaa, R. / Small-angle scattering study of structural changes in the microfibril network of nanocellulose during enzymatic hydrolysis. In: Cellulose. 2013 ; Vol. 20, No. 3. pp. 1031-1040.
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abstract = "The hydrolysis of nanofibrillated cellulose (NFC), consisting of individual cellulose fibrils, was followed using small-angle scattering techniques in order to reveal changes in the substrate structure caused by cellulose degrading enzymes. In particular, the nanoscale structure of the network of cellulose fibrils was characterized with the combination of small-angle neutron scattering and small-angle x-ray scattering. In the nanocellulose with higher xylan content, the interfibrillar distance was shown to remain unchanged during enzymatic degradation, whereas the distance increased in the nanocellulose with lower xylan content. The limiting effect of xylan on the hydrolysis and a faster hydrolysis of the more thoroughly fibrillated segments of the NFC network could be observed. Despite the extensive fibrillation of the raw material, however, the hydrolysis was eventually limited by the aggregated and heterogeneous structure of the substrate.",
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Penttilä, PA, Várnai, A, Fernández, M, Kontro, I, Liljeström, V, Lindner, P, Siika-aho, M, Viikari, L & Serimaa, R 2013, 'Small-angle scattering study of structural changes in the microfibril network of nanocellulose during enzymatic hydrolysis', Cellulose, vol. 20, no. 3, pp. 1031-1040. https://doi.org/10.1007/s10570-013-9899-1

Small-angle scattering study of structural changes in the microfibril network of nanocellulose during enzymatic hydrolysis. / Penttilä, P.A. (Corresponding Author); Várnai, A.; Fernández, M.; Kontro, I.; Liljeström, V.; Lindner, P.; Siika-aho, Matti; Viikari, L.; Serimaa, R.

In: Cellulose, Vol. 20, No. 3, 2013, p. 1031-1040.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Small-angle scattering study of structural changes in the microfibril network of nanocellulose during enzymatic hydrolysis

AU - Penttilä, P.A.

AU - Várnai, A.

AU - Fernández, M.

AU - Kontro, I.

AU - Liljeström, V.

AU - Lindner, P.

AU - Siika-aho, Matti

AU - Viikari, L.

AU - Serimaa, R.

PY - 2013

Y1 - 2013

N2 - The hydrolysis of nanofibrillated cellulose (NFC), consisting of individual cellulose fibrils, was followed using small-angle scattering techniques in order to reveal changes in the substrate structure caused by cellulose degrading enzymes. In particular, the nanoscale structure of the network of cellulose fibrils was characterized with the combination of small-angle neutron scattering and small-angle x-ray scattering. In the nanocellulose with higher xylan content, the interfibrillar distance was shown to remain unchanged during enzymatic degradation, whereas the distance increased in the nanocellulose with lower xylan content. The limiting effect of xylan on the hydrolysis and a faster hydrolysis of the more thoroughly fibrillated segments of the NFC network could be observed. Despite the extensive fibrillation of the raw material, however, the hydrolysis was eventually limited by the aggregated and heterogeneous structure of the substrate.

AB - The hydrolysis of nanofibrillated cellulose (NFC), consisting of individual cellulose fibrils, was followed using small-angle scattering techniques in order to reveal changes in the substrate structure caused by cellulose degrading enzymes. In particular, the nanoscale structure of the network of cellulose fibrils was characterized with the combination of small-angle neutron scattering and small-angle x-ray scattering. In the nanocellulose with higher xylan content, the interfibrillar distance was shown to remain unchanged during enzymatic degradation, whereas the distance increased in the nanocellulose with lower xylan content. The limiting effect of xylan on the hydrolysis and a faster hydrolysis of the more thoroughly fibrillated segments of the NFC network could be observed. Despite the extensive fibrillation of the raw material, however, the hydrolysis was eventually limited by the aggregated and heterogeneous structure of the substrate.

KW - bioethanol

KW - cellulose

KW - enzymatic hydrolysis

KW - hemicellulase

KW - SANS

KW - SAXS

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DO - 10.1007/s10570-013-9899-1

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JO - Cellulose

JF - Cellulose

SN - 0969-0239

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