Snapshot of virus evolution in hypersaline environments from the characterization of a membrane-containing Salisaeta icosahedral phage 1

Antti P Aalto, David Bitto, Janne J Ravantti, Dennis H Bamford, Juha T Huiskonen, Hanna M Oksanen

Research output: Contribution to journalArticleScientificpeer-review

32 Citations (Scopus)

Abstract

The multitude of archaea and bacteria inhabiting extreme environments has only become evident during the last decades. As viruses apply a significant evolutionary force to their hosts, there is an inherent value in learning about viruses infecting these extremophiles. In this study, we have focused on one such unique virus-host pair isolated from a hypersaline environment: an icosahedral, membrane-containing double-stranded DNA virus--Salisaeta icosahedral phage 1 (SSIP-1) and its halophilic host bacterium Salisaeta sp. SP9-1 closely related to Salisaeta longa. The architectural principles, virion composition, and the proposed functions associated with some of the ORFs of the virus are surprisingly similar to those found in viruses belonging to the PRD1-adenovirus lineage. The virion structure, determined by electron cryomicroscopy, reveals that the bulk of the outer protein capsid is composed of upright standing pseudohexameric capsomers organized on a T = 49 icosahedral lattice. Our results give a comprehensive description of a halophilic virus-host system and shed light on the relatedness of viruses based on their virion architecture.

Original languageEnglish
Pages (from-to)7079-84
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number18
DOIs
Publication statusPublished - 1 May 2012
MoE publication typeA1 Journal article-refereed

Keywords

  • Bacteriophages/genetics
  • Bacteroidetes/virology
  • Base Sequence
  • Capsid/ultrastructure
  • Cryoelectron Microscopy
  • DNA, Viral/genetics
  • Environment
  • Evolution, Molecular
  • Genome, Viral
  • Host-Pathogen Interactions
  • Imaging, Three-Dimensional
  • Molecular Sequence Data
  • Open Reading Frames
  • Saline Solution, Hypertonic
  • Virus Integration

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