Solucel® – a functional genomics approach toward the understanding of plant secondary metabolism

    Research output: Contribution to conferenceConference PosterScientific

    Abstract

    Our newly developed technology platform - SOLUCEL® - facilitates the discovery of differentially expressed genes in any plant system without pre-existing sequence knowledge. Coupling transcriptomics based on cDNA-AFLP transcript profiling and the high-throughput functional analysis of genes, this method allows overcoming classical problems in the use of plant cell cultures for the production of valuable secondary compounds. So far we have monitored jasmonate-induced changes on the transcript and metabolite profiles of Nicotiana tabacum BY-2 and Catharanthus roseus cell cultures. An inventory of hundreds of genes, regulatory and structural, which are potentially involved in the secondary metabolism has been built. For example more than 50 tobacco BY-2 full-length open reading frames have been isolated in a high-throughput manner. These are now functionally tested applying LC-MS techniques by homologous overexpression and are used for combinatorial biochemistry by heterologous expression in transgenic Hyoscyamus muticus and Catharanthus roseus hairy roots.
    Original languageEnglish
    Publication statusPublished - 2004
    MoE publication typeNot Eligible
    EventABIC 2004 AgBiotech goes Europe - Cologne, Germany
    Duration: 12 Sep 200415 Sep 2004

    Conference

    ConferenceABIC 2004 AgBiotech goes Europe
    CountryGermany
    CityCologne
    Period12/09/0415/09/04

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