Our newly developed technology platform - SOLUCEL® - facilitates the discovery of differentially expressed genes in any plant system without pre-existing sequence knowledge. Coupling transcriptomics based on cDNA-AFLP transcript profiling and the high-throughput functional analysis of genes, this method allows overcoming classical problems in the use of plant cell cultures for the production of valuable secondary compounds. So far we have monitored jasmonate-induced changes on the transcript and metabolite profiles of Nicotiana tabacum BY-2 and Catharanthus roseus cell cultures. An inventory of hundreds of genes, regulatory and structural, which are potentially involved in the secondary metabolism has been built. For example more than 50 tobacco BY-2 full-length open reading frames have been isolated in a high-throughput manner. These are now functionally tested applying LC-MS techniques by homologous overexpression and are used for combinatorial biochemistry by heterologous expression in transgenic Hyoscyamus muticus and Catharanthus roseus hairy roots.
|Published - 2004
|MoE publication type
|ABIC 2004 AgBiotech goes Europe - Cologne, Germany
Duration: 12 Sept 2004 → 15 Sept 2004
|ABIC 2004 AgBiotech goes Europe
|12/09/04 → 15/09/04