Solucel® – a functional genomics approach toward the understanding of plant secondary metabolism

Research output: Contribution to conferenceConference PosterScientific

Abstract

Our newly developed technology platform - SOLUCEL® - facilitates the discovery of differentially expressed genes in any plant system without pre-existing sequence knowledge. Coupling transcriptomics based on cDNA-AFLP transcript profiling and the high-throughput functional analysis of genes, this method allows overcoming classical problems in the use of plant cell cultures for the production of valuable secondary compounds. So far we have monitored jasmonate-induced changes on the transcript and metabolite profiles of Nicotiana tabacum BY-2 and Catharanthus roseus cell cultures. An inventory of hundreds of genes, regulatory and structural, which are potentially involved in the secondary metabolism has been built. For example more than 50 tobacco BY-2 full-length open reading frames have been isolated in a high-throughput manner. These are now functionally tested applying LC-MS techniques by homologous overexpression and are used for combinatorial biochemistry by heterologous expression in transgenic Hyoscyamus muticus and Catharanthus roseus hairy roots.
Original languageEnglish
Publication statusPublished - 2004
MoE publication typeNot Eligible
EventABIC 2004 AgBiotech goes Europe - Cologne, Germany
Duration: 12 Sep 200415 Sep 2004

Conference

ConferenceABIC 2004 AgBiotech goes Europe
CountryGermany
CityCologne
Period12/09/0415/09/04

Fingerprint

Catharanthus roseus
cell culture
Hyoscyamus muticus
genomics
metabolism
structural genes
regulator genes
transcriptomics
biochemistry
Nicotiana tabacum
amplified fragment length polymorphism
open reading frames
tobacco
genes
metabolites
gene expression
methodology

Cite this

Rischer, H., Häkkinen, S. T., Seppänen-Laakso, T., Goossens, A., De Sutter, V., Inzé, D., & Oksman-Caldentey, K-M. (2004). Solucel® – a functional genomics approach toward the understanding of plant secondary metabolism. Poster session presented at ABIC 2004 AgBiotech goes Europe, Cologne, Germany.
Rischer, Heiko ; Häkkinen, Suvi T. ; Seppänen-Laakso, Tuulikki ; Goossens, Alain ; De Sutter, Valerie ; Inzé, Dirk ; Oksman-Caldentey, Kirsi-Marja. / Solucel® – a functional genomics approach toward the understanding of plant secondary metabolism. Poster session presented at ABIC 2004 AgBiotech goes Europe, Cologne, Germany.
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title = "Solucel{\circledR} – a functional genomics approach toward the understanding of plant secondary metabolism",
abstract = "Our newly developed technology platform - SOLUCEL{\circledR} - facilitates the discovery of differentially expressed genes in any plant system without pre-existing sequence knowledge. Coupling transcriptomics based on cDNA-AFLP transcript profiling and the high-throughput functional analysis of genes, this method allows overcoming classical problems in the use of plant cell cultures for the production of valuable secondary compounds. So far we have monitored jasmonate-induced changes on the transcript and metabolite profiles of Nicotiana tabacum BY-2 and Catharanthus roseus cell cultures. An inventory of hundreds of genes, regulatory and structural, which are potentially involved in the secondary metabolism has been built. For example more than 50 tobacco BY-2 full-length open reading frames have been isolated in a high-throughput manner. These are now functionally tested applying LC-MS techniques by homologous overexpression and are used for combinatorial biochemistry by heterologous expression in transgenic Hyoscyamus muticus and Catharanthus roseus hairy roots.",
author = "Heiko Rischer and H{\"a}kkinen, {Suvi T.} and Tuulikki Sepp{\"a}nen-Laakso and Alain Goossens and {De Sutter}, Valerie and Dirk Inz{\'e} and Kirsi-Marja Oksman-Caldentey",
note = "not in program, poster?; ABIC 2004 AgBiotech goes Europe ; Conference date: 12-09-2004 Through 15-09-2004",
year = "2004",
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Rischer, H, Häkkinen, ST, Seppänen-Laakso, T, Goossens, A, De Sutter, V, Inzé, D & Oksman-Caldentey, K-M 2004, 'Solucel® – a functional genomics approach toward the understanding of plant secondary metabolism' ABIC 2004 AgBiotech goes Europe, Cologne, Germany, 12/09/04 - 15/09/04, .

Solucel® – a functional genomics approach toward the understanding of plant secondary metabolism. / Rischer, Heiko; Häkkinen, Suvi T.; Seppänen-Laakso, Tuulikki; Goossens, Alain; De Sutter, Valerie; Inzé, Dirk; Oksman-Caldentey, Kirsi-Marja.

2004. Poster session presented at ABIC 2004 AgBiotech goes Europe, Cologne, Germany.

Research output: Contribution to conferenceConference PosterScientific

TY - CONF

T1 - Solucel® – a functional genomics approach toward the understanding of plant secondary metabolism

AU - Rischer, Heiko

AU - Häkkinen, Suvi T.

AU - Seppänen-Laakso, Tuulikki

AU - Goossens, Alain

AU - De Sutter, Valerie

AU - Inzé, Dirk

AU - Oksman-Caldentey, Kirsi-Marja

N1 - not in program, poster?

PY - 2004

Y1 - 2004

N2 - Our newly developed technology platform - SOLUCEL® - facilitates the discovery of differentially expressed genes in any plant system without pre-existing sequence knowledge. Coupling transcriptomics based on cDNA-AFLP transcript profiling and the high-throughput functional analysis of genes, this method allows overcoming classical problems in the use of plant cell cultures for the production of valuable secondary compounds. So far we have monitored jasmonate-induced changes on the transcript and metabolite profiles of Nicotiana tabacum BY-2 and Catharanthus roseus cell cultures. An inventory of hundreds of genes, regulatory and structural, which are potentially involved in the secondary metabolism has been built. For example more than 50 tobacco BY-2 full-length open reading frames have been isolated in a high-throughput manner. These are now functionally tested applying LC-MS techniques by homologous overexpression and are used for combinatorial biochemistry by heterologous expression in transgenic Hyoscyamus muticus and Catharanthus roseus hairy roots.

AB - Our newly developed technology platform - SOLUCEL® - facilitates the discovery of differentially expressed genes in any plant system without pre-existing sequence knowledge. Coupling transcriptomics based on cDNA-AFLP transcript profiling and the high-throughput functional analysis of genes, this method allows overcoming classical problems in the use of plant cell cultures for the production of valuable secondary compounds. So far we have monitored jasmonate-induced changes on the transcript and metabolite profiles of Nicotiana tabacum BY-2 and Catharanthus roseus cell cultures. An inventory of hundreds of genes, regulatory and structural, which are potentially involved in the secondary metabolism has been built. For example more than 50 tobacco BY-2 full-length open reading frames have been isolated in a high-throughput manner. These are now functionally tested applying LC-MS techniques by homologous overexpression and are used for combinatorial biochemistry by heterologous expression in transgenic Hyoscyamus muticus and Catharanthus roseus hairy roots.

M3 - Conference Poster

ER -

Rischer H, Häkkinen ST, Seppänen-Laakso T, Goossens A, De Sutter V, Inzé D et al. Solucel® – a functional genomics approach toward the understanding of plant secondary metabolism. 2004. Poster session presented at ABIC 2004 AgBiotech goes Europe, Cologne, Germany.