Solucel® – a functional genomics approach toward the understanding of plant secondary metabolism

Heiko Rischer; Suvi T. Häkkinen; Tuulikki Seppänen-Laakso; Alain Goossens; Valerie De Sutter; Dirk Inzé; Kirsi-Marja Oksman-Caldentey

Solucel® – a functional genomics approach toward the understanding of plant secondary metabolism

Heiko Rischer, Suvi T. Häkkinen, Tuulikki Seppänen-Laakso, Alain Goossens, Valerie De Sutter, Dirk Inzé, Kirsi-Marja Oksman-Caldentey

Research output: Contribution to conference › Conference Poster › Scientific

Abstract

Our newly developed technology platform - SOLUCEL® - facilitates the discovery of differentially expressed genes in any plant system without pre-existing sequence knowledge. Coupling transcriptomics based on cDNA-AFLP transcript profiling and the high-throughput functional analysis of genes, this method allows overcoming classical problems in the use of plant cell cultures for the production of valuable secondary compounds. So far we have monitored jasmonate-induced changes on the transcript and metabolite profiles of Nicotiana tabacum BY-2 and Catharanthus roseus cell cultures. An inventory of hundreds of genes, regulatory and structural, which are potentially involved in the secondary metabolism has been built. For example more than 50 tobacco BY-2 full-length open reading frames have been isolated in a high-throughput manner. These are now functionally tested applying LC-MS techniques by homologous overexpression and are used for combinatorial biochemistry by heterologous expression in transgenic Hyoscyamus muticus and Catharanthus roseus hairy roots.

Original language	English
Publication status	Published - 2004
MoE publication type	Not Eligible
Event	ABIC 2004 AgBiotech goes Europe - Cologne, Germany Duration: 12 Sept 2004 → 15 Sept 2004

Conference

Conference	ABIC 2004 AgBiotech goes Europe
Country/Territory	Germany
City	Cologne
Period	12/09/04 → 15/09/04

Cite this

@conference{48c540c204354144801d741d14c73938,

title = "Solucel{\textregistered} – a functional genomics approach toward the understanding of plant secondary metabolism",

abstract = "Our newly developed technology platform - SOLUCEL{\textregistered} - facilitates the discovery of differentially expressed genes in any plant system without pre-existing sequence knowledge. Coupling transcriptomics based on cDNA-AFLP transcript profiling and the high-throughput functional analysis of genes, this method allows overcoming classical problems in the use of plant cell cultures for the production of valuable secondary compounds. So far we have monitored jasmonate-induced changes on the transcript and metabolite profiles of Nicotiana tabacum BY-2 and Catharanthus roseus cell cultures. An inventory of hundreds of genes, regulatory and structural, which are potentially involved in the secondary metabolism has been built. For example more than 50 tobacco BY-2 full-length open reading frames have been isolated in a high-throughput manner. These are now functionally tested applying LC-MS techniques by homologous overexpression and are used for combinatorial biochemistry by heterologous expression in transgenic Hyoscyamus muticus and Catharanthus roseus hairy roots.",

author = "Heiko Rischer and H{\"a}kkinen, {Suvi T.} and Tuulikki Sepp{\"a}nen-Laakso and Alain Goossens and {De Sutter}, Valerie and Dirk Inz{\'e} and Kirsi-Marja Oksman-Caldentey",

note = "not in program, poster?; ABIC 2004 AgBiotech goes Europe ; Conference date: 12-09-2004 Through 15-09-2004",

year = "2004",

language = "English",

}

TY - CONF

T1 - Solucel® – a functional genomics approach toward the understanding of plant secondary metabolism

AU - Rischer, Heiko

AU - Häkkinen, Suvi T.

AU - Seppänen-Laakso, Tuulikki

AU - Goossens, Alain

AU - De Sutter, Valerie

AU - Inzé, Dirk

AU - Oksman-Caldentey, Kirsi-Marja

N1 - not in program, poster?

PY - 2004

Y1 - 2004

N2 - Our newly developed technology platform - SOLUCEL® - facilitates the discovery of differentially expressed genes in any plant system without pre-existing sequence knowledge. Coupling transcriptomics based on cDNA-AFLP transcript profiling and the high-throughput functional analysis of genes, this method allows overcoming classical problems in the use of plant cell cultures for the production of valuable secondary compounds. So far we have monitored jasmonate-induced changes on the transcript and metabolite profiles of Nicotiana tabacum BY-2 and Catharanthus roseus cell cultures. An inventory of hundreds of genes, regulatory and structural, which are potentially involved in the secondary metabolism has been built. For example more than 50 tobacco BY-2 full-length open reading frames have been isolated in a high-throughput manner. These are now functionally tested applying LC-MS techniques by homologous overexpression and are used for combinatorial biochemistry by heterologous expression in transgenic Hyoscyamus muticus and Catharanthus roseus hairy roots.

AB - Our newly developed technology platform - SOLUCEL® - facilitates the discovery of differentially expressed genes in any plant system without pre-existing sequence knowledge. Coupling transcriptomics based on cDNA-AFLP transcript profiling and the high-throughput functional analysis of genes, this method allows overcoming classical problems in the use of plant cell cultures for the production of valuable secondary compounds. So far we have monitored jasmonate-induced changes on the transcript and metabolite profiles of Nicotiana tabacum BY-2 and Catharanthus roseus cell cultures. An inventory of hundreds of genes, regulatory and structural, which are potentially involved in the secondary metabolism has been built. For example more than 50 tobacco BY-2 full-length open reading frames have been isolated in a high-throughput manner. These are now functionally tested applying LC-MS techniques by homologous overexpression and are used for combinatorial biochemistry by heterologous expression in transgenic Hyoscyamus muticus and Catharanthus roseus hairy roots.

M3 - Conference Poster

T2 - ABIC 2004 AgBiotech goes Europe

Y2 - 12 September 2004 through 15 September 2004

ER -

Solucel® – a functional genomics approach toward the understanding of plant secondary metabolism

Abstract

Conference

Fingerprint

Cite this