Solution structure of nodularin: An inhibitor of serine/threonine-specific protein phosphatases

Arto Annila (Corresponding Author), Jaana Lehtimäki, Kimmo Mattila, John E. Eriksson, Kaarina Sivonen, Tapio T. Rantala, Torbjörn Drakenberg

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Abstract

The three-dimensional solution structure of nodularin was studied by NMR and molecular dynamics simulations. The conformation in water was determined from the distance and dihedral data by distance geometry and refined by iterative relaxation matrix analysis. The cyclic backbone adopts a well defined conformation but the remote parts of the side chains of arginine as well as the amino acid derivative Adda have a large spatial dispersion. For the unusual amino acids the partial charges were calculated and nodularin was subjected to molecular dynamic simulations in water. A good agreement was found between experimental and computational data with hydrogen bonds, solvent accessibility, molecular motion, and conformational exchange. The three-dimensional structure resembles very closely that of microcystin-LR in the chemically equivalent segment. Therefore, it is expected that the binding of both microcystins and nodularins to serine/threonine-specific protein phosphatases is similar on an atomic level.
Original languageEnglish
Pages (from-to)16695-16702
JournalJournal of Biological Chemistry
Volume271
DOIs
Publication statusPublished - 1996
MoE publication typeA1 Journal article-refereed

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Annila, A., Lehtimäki, J., Mattila, K., Eriksson, J. E., Sivonen, K., Rantala, T. T., & Drakenberg, T. (1996). Solution structure of nodularin: An inhibitor of serine/threonine-specific protein phosphatases. Journal of Biological Chemistry, 271, 16695-16702. https://doi.org/10.1074/jbc.271.28.16695